Abstract
Suspicious hybrids of painted storks and milky storks were found in a Malaysian zoo. Blood of these birds was sampled on FTA cards for DNA fingerprinting. Of 44 optimized primers, 6 produced diagnostic markers that could identify hybrids. The markers were based on simple, direct PCR-generated multilocus banding patterns that provided two sets of genetic data, one for each of the two stork species and another for the hybrids. It also revealed that large DNA fragments (3,000 bp) could be amplified from blood collected on FTA cards. When the results of each individual bird’s DNA fingerprint were compared with plumage characters, the hybrids were found to express a range of intermediate phenotypic traits of the pure breeds with no dominant plumage characteristic from either parental species.
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Acknowledgments
This project was funded mainly by a Fundamental Research Grant of the Ministry of Higher Education, Malaysia, entitled “Molecular population genetics of the milky stork and the painted stork found in Malaysia,” headed by S. G. Tan, and partially by the Malaysian Wildlife Department. Our special thanks go to the veterinarian team of the Malaysian Wildlife Department for samples of stork blood on FTA cards and to the staff of the Bird’s House in the Malaysian National Zoo, Kuala Lumpur, for providing information on the morphologies of the stork samples used in the study and for helping in the blood sampling of these storks.
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Yee, E.Y.S., Zainuddin, Z.Z., Ismail, A. et al. Identification of Hybrids of Painted and Milky Storks Using FTA Card-Collected Blood, Molecular Markers, and Morphologies. Biochem Genet 51, 789–799 (2013). https://doi.org/10.1007/s10528-013-9607-8
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DOI: https://doi.org/10.1007/s10528-013-9607-8