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Lead-induced increase in antioxidant enzymes and lipid peroxidation products in developing rat brain

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Abstract

Pregnant rats were treated with 0.4% lead acetate through drinking water from 6th day of gestation and this treatment was continued till 21 post natal days (PND). Four regions of the brain namely hippocampus, cerebellum, frontal cortex and brain stem were dissected at 10, 20, 30 and 40 PND for estimation of lipid peroxidation products (LPP), catalase (CAT) and superoxide dismutase (SOD). The results indicate that there was a significant (P < 0.05) increase of LPP in exposed rats than their corresponding control at 10, 20 and 30 PND both in hippocampus and cerebellum. At PND 40, the LPP of control and exposed were found to be almost same in both the tissues indicating recovery from lead toxicity. CAT activity was significantly (P < 0.05) high in hippocampus of exposed rats up to PND 30 but up to PND 20 in cerebellum and frontal cortex. However, in brain stem, a significant (P < 0.05) increase in CAT activity was observed only at PND 10. A significant (P < 0.05) increase in SOD activity was observed up to PND 30 both in hippocampus and cerebellum on lead exposure. Frontal cortex exhibited a similar significant (P < 0.05) increase of SOD activity up to PND 20 and for brain stem up to PND 10. There was no significant change in the activity of antioxidant enzymes (CAT and SOD) and LPP in all the four brain tissues of control and exposed rats at PND 40 indicating recovery from lead-induced oxidative stress.

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Acknowledgements

This research work was supported by NIH/FIC/MIRT #00132, NIH/NIGMS/MBRS-SCORE #GM55356 awarded to Bettaiya Rajanna at Alcorn State University, Mississippi, USA. Erika Brown and Rashidi McCormick are undergraduate student participants in MIRT Program. The authors thank the authorities of Andhra University for providing research facilities for this international collaboration of undergraduate student research training program.

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Authors and Affiliations

  1. Department of Zoology, Andhra University, Visakhapatnam, 530 003, India

    Kiran Kumar Bokara & Prabhakara Rao Yallapragada

  2. Department of Biological Sciences, Alcorn State University, Lorman, MS, USA

    Erika Brown, Rashidi McCormick, Sharada Rajanna & Rajanna Bettaiya

Authors
  1. Kiran Kumar Bokara
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  2. Erika Brown
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  3. Rashidi McCormick
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  4. Prabhakara Rao Yallapragada
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  5. Sharada Rajanna
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  6. Rajanna Bettaiya
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Corresponding author

Correspondence to Rajanna Bettaiya.

Additional information

This research work was presented as a poster in Annual Biomedical Research Conference for Minority Students (ABRCMS) at Dallas, Texas, USA, during November 10–13, 2004 and the abstract was printed on page 231 of the Conference Proceedings

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Bokara, K.K., Brown, E., McCormick, R. et al. Lead-induced increase in antioxidant enzymes and lipid peroxidation products in developing rat brain. Biometals 21, 9–16 (2008). https://doi.org/10.1007/s10534-007-9088-5

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  • DOI: https://doi.org/10.1007/s10534-007-9088-5

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