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Journal of Inherited Metabolic Disease

Erschienen in:

01.06.2011 | SSIEM Symposium 2010

On the future of “omics”: lipidomics

verfasst von: William J. Griffiths, Michael Ogundare, Christopher M. Williams, Yuqin Wang

Erschienen in: Journal of Inherited Metabolic Disease | Ausgabe 3/2011

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Abstract

Summary

Following in the wake of the genomic and proteomic revolutions new fields of “omics” research are emerging. The metabolome provides the natural complement to the genome and proteome, however, the extreme physicochemical diversity of the metabolome leads to a subdivision of metabolites into compounds soluble in aqueous solutions or those soluble in organic solvents. A complete molecular and quantitative investigation of the latter when isolated from tissue, fluid or cells constitutes lipidomics. Like proteomics, lipidomics is a subject which is both technology driven and technology driving, with the primary technologies being mass spectrometry, with or without on-line chromatography and computer-assisted data analysis. In this paper we will examine the underlying fundamentals of different lipidomic experimental approaches including the “shotgun” and “top-down” global approaches, and the more targeted liquid chromatography – or gas chromatography – mass spectrometry approaches. Application of these approaches to the identification of in-born errors of metabolism will be discussed.

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Titel: On the future of “omics”: lipidomics
verfasst von: William J. Griffiths
Michael Ogundare
Christopher M. Williams
Yuqin Wang
Publikationsdatum: 01.06.2011
Verlag: Springer Netherlands
Erschienen in: Journal of Inherited Metabolic Disease / Ausgabe 3/2011
Print ISSN: 0141-8955
Elektronische ISSN: 1573-2665
DOI: https://doi.org/10.1007/s10545-010-9274-4

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