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Clinical & Experimental Metastasis

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01.03.2007 | Research Paper

Major but not minor hepatectomy accelerates engraftment of extrahepatic tumor cells

verfasst von: Kathrin Rupertus, Otto Kollmar, Claudia Scheuer, Bastian Junker, Michael D. Menger, Martin K. Schilling

Erschienen in: Clinical & Experimental Metastasis | Ausgabe 1/2007

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Abstract

Background

The effect of hepatectomy and hepatic regeneration on intra- and extrahepatic tumor growth is still controversially discussed. Herein we studied the effect of minor (30%) or major (70%) hepatectomy on engraftment of extrahepatic tumor cells, and the role of tumor neovascularization and tumor cell migration.

Methods

Green fluorescent protein (GFP)-transfected CT26.WT colorectal cancer cells were implanted in dorsal skinfold chambers of syngeneic BALB/c mice. Animals underwent 30% (30%Phx, n = 8) or 70% hepatectomy (70%Phx, n = 8). Sham-operated animals served as controls (n = 8). Angiogenesis and neovascularization as well as tumor cell migration, proliferation and growth were studied over 14 days using intravital fluorescence microscopy, histology and immunohistochemistry.

Results

After both minor and major hepatectomy tumor proliferating cell nuclear antigen (PCNA) expression increased significantly (P < 0.05) when compared with nonhepatectomized controls. However, only major but not minor hepatectomy accelerated neovascularization (P < 0.05) and tumor cell migration (P < 0.05). This was associated with a significantly (P < 0.05) enhanced tumor growth after 70%Phx when compared with 30%Phx and controls. The rate of apoptotic cell death was not affected by major or minor hepatectomy.

Conclusion

Regeneration after major hepatectomy accelerates extrahepatic tumor cell engraftment, most probably by acceleration of neovascularization and induction of tumor cell migration.

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Titel: Major but not minor hepatectomy accelerates engraftment of extrahepatic tumor cells
verfasst von: Kathrin Rupertus
Otto Kollmar
Claudia Scheuer
Bastian Junker
Michael D. Menger
Martin K. Schilling
Publikationsdatum: 01.03.2007
Verlag: Kluwer Academic Publishers
Erschienen in: Clinical & Experimental Metastasis / Ausgabe 1/2007
Print ISSN: 0262-0898
Elektronische ISSN: 1573-7276
DOI: https://doi.org/10.1007/s10585-006-9054-6

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23.04.2024 | Medikamente in Schwangerschaft und Stillzeit | Nachrichten

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

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Major but not minor hepatectomy accelerates engraftment of extrahepatic tumor cells

Abstract

Background

Methods

Results

Conclusion

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Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

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Abstract

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Methods

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