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Evaluating osteochondral defect repair potential of autologous rabbit bone marrow cells on type II collagen scaffold

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Abstract

The feasibility of using genipin cross-linked type II collagen scaffold with rabbit bone marrow mesenchymal stem cells (RBMSCs) to repair cartilage defect was herein studied. Induction of RBMSCs into chondrocytic phenotype on type II collagen scaffold in vitro was conducted using TGF-β 3 containing medium. After 3-weeks of induction, chondrocytic behavior, including marker genes expression and specific extracellular matrix (ECM) secretion, was observed. In the in vivo evaluation experiment, the scaffolds containing RBMSCs without prior induction were autologous implanted into the articular cartilage defects made by subchondral drilling. The repairing ability was evaluated. After 2 months, chondrocyte-like cells with lacuna structure and corresponding ECM were found in the repaired sites without apparent inflammation. After 24 weeks, we could easily find cartilage structure the same with normal cartilage in the repair site. In conclusion, it was shown that the scaffolds in combination of in vivo conditions can induce RBMSCs into chondrocytes in repaired area and would be a possible method for articular cartilage repair in clinic and cartilage tissue engineering.

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Acknowledgments

This research is supported by the Ministry of Economic Affairs, Taiwan, under the Technology Development Program for Academia grant 91-EC-17-A-17-S1-0009.

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Authors and Affiliations

  1. Department of Chemical Engineering, National Tsing Hua University, 101, Kuang-Fu Road, Hsin-Chu City, 300, Taiwan

    Wei-Chuan Chen & I-Ming Chu

  2. Department of Chemical Engineering and Materials Science, Yuan Ze University, Chung-Li, Taoyuan, 320, Taiwan

    Chao-Ling Yao

  3. Graduate School of Biotechnology and Bioengineering, Yuan Ze University, Chung-Li, Taoyuan, 320, Taiwan

    Yu-Hong Wei

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  1. Wei-Chuan Chen
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  2. Chao-Ling Yao
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  3. Yu-Hong Wei
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  4. I-Ming Chu
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Correspondence to Yu-Hong Wei or I-Ming Chu.

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Chen, WC., Yao, CL., Wei, YH. et al. Evaluating osteochondral defect repair potential of autologous rabbit bone marrow cells on type II collagen scaffold. Cytotechnology 63, 13–23 (2011). https://doi.org/10.1007/s10616-010-9314-9

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  • DOI: https://doi.org/10.1007/s10616-010-9314-9

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