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Erschienen in: Digestive Diseases and Sciences 9/2006

01.09.2006 | Original Paper

Confirmation and Prevention of Intestinal Barrier Dysfunction and Bacterial Translocation Caused by Methotrexate

verfasst von: Desheng Song, Bin Shi, Hua Xue, Yousheng Li, Xiaodong Yang, Baojun Yu, Zhe Xu, Fukun Liu, Jieshou Li

Erschienen in: Digestive Diseases and Sciences | Ausgabe 9/2006

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Abstract

When intestinal barrier function is damaged bacterial translocation (BT) can occur. The injury to intestinal barrier function caused by chemotherapy has been investigated in some studies, however, definitive evidence of BT caused by chemotherapy is lacking. The aim of this study was to investigate small intestinal barrier dysfunction and BT and to evaluate the preventive effect of granulocyte colony-stimulating factor (G-CSF) on intestinal barrier dysfunction and BT in a rat model of chemotherapy. Sprague-Dawley rats were treated with methotrexate (MTX; 3.5 mg/kg) for 3 days to induce intestinal barrier dysfunction and BT, gavaged Escherichia coli TG1 labeled with green fluorescent protein for 2 days to track BT, and G-CSF (10 μg/kg) for 4 days to prevent intestinal barrier dysfunction and BT. Intestinal permeability was measured by the urinary excretion rate of lactulose and mannitol following administration by gavage. Representative tissue specimens from the mesenteric lymph nodes, spleen, liver, and kidney were aseptically harvested for bacteria culture in ampicillin-supplemented medium. Light microscopy was performed on intestinal samples. MTX induced significant mucosal and villous atrophy in ileum and significantly increased intestinal permeability. MTX also induced noticeable BT. G-CSF significantly increased the mucosal thickness and villous height of the ileum and decreased intestinal permeability and BT. In conclusion, MTX caused intestinal barrier dysfunction and BT, and G-CSF prevented intestinal barrier dysfunction and BT.
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Metadaten
Titel
Confirmation and Prevention of Intestinal Barrier Dysfunction and Bacterial Translocation Caused by Methotrexate
verfasst von
Desheng Song
Bin Shi
Hua Xue
Yousheng Li
Xiaodong Yang
Baojun Yu
Zhe Xu
Fukun Liu
Jieshou Li
Publikationsdatum
01.09.2006
Verlag
Springer US
Erschienen in
Digestive Diseases and Sciences / Ausgabe 9/2006
Print ISSN: 0163-2116
Elektronische ISSN: 1573-2568
DOI
https://doi.org/10.1007/s10620-005-9058-0

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