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Digestive Diseases and Sciences

Erschienen in:

01.01.2007 | Original Paper

Rebamipide Inhibits Gastric Cancer Cell Growth

verfasst von: Tetsuya Tanigawa, Rama Pai, Tetsuo Arakawa, Andrzej S. Tarnawski

Erschienen in: Digestive Diseases and Sciences | Ausgabe 1/2007

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Abstract

Rebamipide is an antiulcer drug used in Japan, Korea, China, Philippines, and other Asian countries for treatment of gastritis and peptic ulcer. Its effect on gastric cancer cell growth and its regulatory mechanisms remain unknown. We examined whether rebamipide affects human gastric cancer cell proliferation and activation of Smad signaling pathway. Gastric cancer (AGS) cells were treated with either (a) medium (control), (b) medium-containing rebamipide (0.5–2 mg/mL), or (c) PD98059+rebamipide. We determined cell proliferation, expression of p21, phosphorylation of ERK2, JNK p38, and Smad2/3, formation of Smad2/3–Smad4 complex, and nuclear translocation of Smad2/3. Rebamipide treatment inhibited AGS cell proliferation and increased p21, Smad2/3 phosphorylation, and Smad2/3–Smad4 complex formation. Rebamipide induced phosphorylation of ERK2 but not JNK or p38. Inactivation of ERK2 by PD98059 partly attenuated rebamipide-induced p21 expression. These data demonstrate that rebamipide activates Smad signaling pathway and suppresses human gastric cancer cell growth. Inactivation of ERK2 partly inhibited rebamipide-induced p21 expression, indicating a crosstalk between ERK and Smad signaling pathways.

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Titel: Rebamipide Inhibits Gastric Cancer Cell Growth
verfasst von: Tetsuya Tanigawa
Rama Pai
Tetsuo Arakawa
Andrzej S. Tarnawski
Publikationsdatum: 01.01.2007
Verlag: Springer US
Erschienen in: Digestive Diseases and Sciences / Ausgabe 1/2007
Print ISSN: 0163-2116
Elektronische ISSN: 1573-2568
DOI: https://doi.org/10.1007/s10620-006-9226-x

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