nach oben

Erschienen in:

01.12.2006 | Original Paper

Transepithelial Leak in Barrett’s Esophagus

verfasst von: J. M. Mullin, M. C. Valenzano, S. Trembeth, P. D. Allegretti, J. J. Verrecchio, J. D. Schmidt, V. Jain, J. B. Meddings, G. Mercogliano, J. J. Thornton

Erschienen in: Digestive Diseases and Sciences | Ausgabe 12/2006

Einloggen, um Zugang zu erhalten

Abstract

Using orally administered sucrose as a probe of gastrointestinal permeability, this study focused on determining whether Barrett’s metaplasia exhibits a paracellular transepithelial leak to small nonelectrolytes. Subjects in five separate classes (nonendoscoped, asymptomatic controls; endoscoped, asymptomatic controls; gastroesophageal reflux disease without mucosal complications; grossly visible esophagitis; and Barrett’s esophagus) consumed a sucrose solution at bedtime and collected all overnight urine. Urine volume was measured and sucrose concentration was determined by high-performance liquid chromatography. Patients with Barrett’s were observed to exhibit a transepithelial leak to sucrose whose mean value was threefold greater than that seen in healthy control subjects or patients with reflux but without any mucosal defect. A parallel study of claudin tight junction proteins in endoscopy biopsy samples showed that whereas Barrett’s metaplasia contains dramatically more claudin-2 and claudin-3 than is found in normal esophageal mucosa, it is markedly lower in claudins 1 and 5, indicating very different tight junction barriers.

Martinez-Palomo A (1970) Ultrastructural modifications of intercellular junctions between tumor cells. In Vitro 6:15–20PubMed

Alroy J (1979) Ultrastructure of canine urinary bladder carcinoma. Vet Pathol 16:693–701PubMed

Robenek H, Schopper C, Fasske E, Fetting R, Themann H (1981) Structure and function of the junctional complement of spontaneous and transplanted muring mammary carcinomas. J Submicrosc Cytol 13:347–363PubMed

Kerjaschki D, Krisch K, Sleyter U, Umrath W, Jakesz R, Depisch D, Kokoschka R, Horandner H (1979) The structure of tight junctions in human thyroid tumors. A systematic freeze-fracture study. Am J Pathol 96:207–225PubMed

Polak-Charcon S, Shoham J, Ben-Shaul Y (1980) Tight junctions of epithelial cells of human fetal hindgut, normal colon and colon adenocarcinoma. J Natl Cancer Inst 65:53–57PubMed

Aoyagi K, Kohfiji K, Yano S, Murakami N, Hori H, Terasaki Y, Takeda J, Tanaka M, Shirouzu K (2000) Morphological change in the MNNG-treated rat gastric mucosa. Kurume Med J 47:31–36PubMed

Saito T (1984) Ultrastructural changes on the junctional complexes in the human urinary bladder carcinoma by thin sectioning and freeze fracture. J Clin Electron Micros 17:201–209

Swift JG, Mukherjee TM, Rowland R (1983) Intercellular junctions in hepatocellular carcinoma. J Submicrosc Cytol 15:799–810PubMed

Soler AP, Miller RD, Laughlin KV, Carp NZ, Klurfeld DM, Mullin JM (1999) Increased tight junctional permeability is associated with the development of colon cancer. Carcinogenesis 20:1425–1431CrossRefPubMed

10.

Davies R, Joseph R, Asbun H, Sedwitz M (1989) Detection of the cancer-prone colon, using transepithelial impedance analysis. Arch Surg 124:480–484PubMed

11.

Gonzalez-Mariscal L, Betanzos A, Nava P, Jaramillo B (2003) Tight junction proteins. Prog Biophys Mol Biol 81:1–44CrossRefPubMed

12.

Hollander D (1988) Crohn’s disease: a permeability disorder of the tight junction. Gut 29:1621–1624PubMed

13.

Soderholm JD, Olaison G, Peterson KH, Franzen LE, Lindmark T, Wiren M, Tagesson C, Sjodahl R (2002) Augmented increase in tight junction permeability by luminal stimuli in the non-inflamed ileum of Crohn’s disease. Gut 50:307–313CrossRefPubMed

14.

Mullin JM, Peralta Soler A, Laughlin KV, Kampherstein JA, Russo LM, Saladik DT, George K, Shurina RD, O’Brien TG (1996) Chronic exposure of LLC-PK1 epithelia to the phorbol ester TPA produces polyp-like foci with leaky tight junctions and altered protein kinase C-alpha expression and localization. Exp Cell Res 227:12–22CrossRefPubMed

15.

Guan Y, Woo P, Rubenstein N, Firestone G (2002) Transforming growth factor-alpha abrogates the glucocorticoid stimulation of tight junction formation and reverses the steroid-induced down-regulation of fascin in rat mammary epithelial tumor cells by a Ras-dependent pathway. Exp Cell Res 273:1–11CrossRefPubMed

16.

Chen Y, Lu Q, Schneeberger E, Goodenough DA (2000) Restoration of tight junction structure and barrier function by down-regulation of the mitogen-activated protein kinase pathway in ras-transformed Madin-Darby canine kidney cells. Mol Biol Cell 11:849–862PubMed

17.

Li D, Mrsny RJ (2000) Oncogenic Raf-1 disrupts epithelial tight junctions via downregulation of occludin. J Cell Biol 148:791–800CrossRefPubMed

18.

Mullin JM, Leatherman JM, Valenzano MC, Huerta ER, Verrechio J, Smith DM, Snetselaar K, Liu M, Francis MK, Sell C (2005) Ras mutation impairs epithelial barrier function to a wide range of nonelectrolytes. Mol Biol Cell 16:5538–5550CrossRefPubMed

19.

Nunbhakdi-Craig V, Craig L, Machleidt T, Sontag E (2003) Simian virus 40 small tumor antigen induces deregulation of the actin cytoskeleton and tight junctions in kidney epithelial cells. J Virol 77:2807–2818CrossRefPubMed

20.

Jiang W, Martin T, Matsumoto K, Nakamura T, Mansel R (1999) Hepatocyte growth factor/scatter factor decreases the expression of occluding and transendothelial resistance (TER) and increases paracellular permeability in human vascular endothelial cells. J Cell Physiol 181:319–329CrossRefPubMed

21.

Blanco D, Vicent S, Elizegi E, Pino I, Fraga M, Esteller M, Saffiotti U, Lecanda F, Montuenga L (2004) Altered expression of adhesion molecules and epithelial-mesenchymal transition in silica-induced rat lung carcinogenesis. Lab Invest 84:999–1012CrossRefPubMed

22.

Resnick MB, Konkin T, Routhier J, Sabo E, Pricolo VE (2004) Claudin-1 is a strong prognostic indicator in stage II colonic cancer: a tissue microarray study. Mod Pathol 18(4):511–518

23.

Tobioka H, Isomura H, Kokai Y, Sawada N (2002) Polarized distribution of carcinoembryonic antigen is associated with a tight junction molecule in human colorectal adenocarcinoma. J Pathol 198:207–212CrossRefPubMed

24.

Tobioka H, Isomura H, Kokai Y, Tokunaga Y, Yamaguchi J, Sawada N (2004) Occludin expression decreases with the progression of human endometrial carcinoma. Hum Pathol 35:159–164CrossRefPubMed

25.

Busch C, Hanssen T, Wagener C, O’Brink B (2002) Down-regulation of CEACAM1 in human prostate cancer: correlation with loss of cell polarity, increased proliferation rate, and Gleason grade 3 to 4 transition. Hum Pathol 33:290–298CrossRefPubMed

26.

Kimura Y, Shiozaki H, Hirao M, Maeno Y, Doki Y, Inoue M, Monden T, Ando-Akatsuka Y, Furuse M, Tsukita S, Monden M (1997) Expression of occludin, tight-junction-associated protein, in human digestive tract. Am J Pathol 151:45–54PubMed

27.

Marzioni D, Banita M, Felici A, Paradinas FJ, Newlands E, De Nictolis M, Muhlhauser J, Castellucci M (2001) Expression of ZO-1 and occludin in normal human placenta and in hydatidiform moles. Mol Hum Reprod 7:279–285CrossRefPubMed

28.

Davies R (2002) Blood-brain barrier breakdown in septic encephalopathy and brain tumours. J Anat 200:639–646CrossRefPubMed

29.

Papadopoulos MC, Saadoun S, Woodrow CJ, Davies DC, Costa-Martins P, Moss RF, Krishna S, Bell BA (2001) Occludin expression in microvessels of neoplastic and non-neoplastic human brain. Neuropathol Appl Neurobiol. 27:384–395CrossRefPubMed

30.

Furuse M, Sasaki H, Tsukita S (1999) Manner of interaction of heterogeneous claudin species within and between tight junction strands. J Cell Biol 147:891–903CrossRefPubMed

31.

Liebner S, Kniesel U, Kalbacher H, Wolburg H (2000) Correlation of tight junction morphology with the expression of tight junction proteins in blood-brain barrier endothelial cells. Eur J Cell Biol 79:707–717CrossRefPubMed

32.

Kramer F, White K, Kubbies M, Swisshelm K, Weber BH (2000) Genomic organization of claudin-1 and its assessment in hereditary and sporadic breast cancer. Hum Genet 107:249–256CrossRefPubMed

33.

Willott E, Balda MS, Fanning AS, Jameson B, Van Itallie CM, Anderson JM (1993) The tight junction protein ZO-1 is homologous to the Drosophila discs-large tumor suppressor protein of septate junctions. Proc Natl Acad Sci USA 90:7834–7838CrossRefPubMed

34.

Miwa N, Furuse M, Tsukita S, Niikawa N, Nakamura Y, Furukawa Y (2000) Involvement of claudin-1 in the beta-catenin/Tcf signaling pathway and its frequent upregulation in human colorectal cancers. Oncol Res 12:469–476

35.

Folpe A, Billings S, McKenney J, Walsh S, Nusrat A, Weiss S (2002) Expression of claudin-1, a recently described tight junction-associated protein, distinguishes soft tissue perineurioma from potential mimics. Am J Surg Pathol 12:1620–1626CrossRef

36.

Kominsky SL, Argani P, Korz D, Evron E, Raman V, Garrett E, Rein A, Sauter G, Kallioniemi OP, Sukumar S (2003) Loss of the tight junction protein claudin-7 correlates with histological grade in both ductal carcinoma in situ and invasive ductal carcinoma of the breast. Oncogene 22:2021–2033CrossRefPubMed

37.

Rangel LB, Agarwal R, D’Souza T, Pizer ES, Alo PL, Lancaster WD, Gregoire L, Schwartz DR, Cho KR, Morin PJ (2003) Tight junction proteins claudin-3 and claudin-4 are frequently overexpressed in ovarian cancer but not in ovarian cystadenomas. Clin Cancer Res 9:2567–2575PubMed

38.

Brown LM, Devesa SS (2002) Epidemiologic trends in esophageal and gastric cancer in the United States. Surg Oncol Clin North Am 11:235–256CrossRef

39.

Eloubeidi MA, Homan RK, Martz MD, Theobold KE, Provenzale D (1999) A cost analysis of outpatient care for patients with Barrett’s esophagus in a managed care setting. Am J Gastroenterol 94:2033–2036CrossRefPubMed

40.

Fitzgerald RC (2005) Genetics and prevention of esophageal cancer. Rec Res Cancer Res 166:35–46CrossRef

41.

Sampliner RE (2005) Epidemiology, pathophysiology, and treatment of Barrett’s esophagus: reducing mortality from esophageal adenocarcinoma. Med Clin North Am 1989:293–312CrossRef

42.

Drewitz DJ, Sampliner RE, Garewal HS (1997) The incidence of adenocarcinoma in Barrett’s esophagus: a prospective study of 170 patients followed 4.8 years. Am J Gastroenterol 92:212–215PubMed

43.

Shaheen NJ, Crosby MA, Bozymski EM (2000) Is there publication bias in the reporting of cancer risk of Barrett’s esophagus? Gastroenterology 119:333–338CrossRefPubMed

44.

Mullin JM, Kampherstein JA, Laughlin KV, Saladik DT, Peralta Soler A (1997) Transepithelial paracellular leakiness induced by chronic phorbol ester exposure correlates with polyp-like foci and redistribution of protein kinase C-alpha. Carcinogenesis 18:2339–2345CrossRefPubMed

45.

Meddings JB, Sutherland LR, Byles NI, Wallace JL (1993) Sucrose, a novel permeability marker for gastroduodenal disease. Gastroenterology 104:1619–1626PubMed

46.

Kawabata H, Meddings JB, Uchida Y, Matsuda K, Sasahara K, Nishioka M (1998) Sucrose permeability as a means of detecting diseases of the upper digestive tract. J Gastroenterol Hepatol 13:1002–1006PubMed

47.

Subramanyam K, Patterson M, Gourley WK (1985) Healing of endoscopic biopsy sites in the human rectum. J Clin Gastroenterol 7:266–268CrossRefPubMed

48.

Mullin JM, Agostino N, Rendon-Huerta E, Thornton JJ (2005) Keynote review: epithelial and endothelial barriers in human disease. Drug Discov Today 10:395–408CrossRefPubMed

49.

Rendon-Huerta E, Valenzano MC, Mullin JM, Trembeth SE, Kothari RH, Hameed K, Mercogliano G, Thornton JJ (2003) Comparison of three integral tight junction barrier proteins in Barrett’s epithelium versus normal esophageal epithelium. Am J Gastroenterol 98:1901CrossRefPubMed

50.

Munck BG, Rasmussen SN (1977) Paracellular permeability of extracellular space markers across rat jejunum in vitro. Indication of a transepithelial fluid circuit. J Physiol 271: 473–488PubMed

51.

Gonzalez-Correa C, Brown B, Smallwood R, Stephenson T, Stoddard C, Bardhan K (2003) Low frequency electrical bioimpedance for the detection of inflammation and dysplasia in Barrett’s oesophagus. Physiol Meas 24:291–296CrossRefPubMed

52.

Tobey NA, Carson JL, Alkiek RA, Orlando RC (1996) Dilated intercellular spaces: a morphological feature of acid reflux-damaged human esophageal epithelium. Gastroenterology 111:1200–1205CrossRefPubMed

53.

Tobey NA, Hosseini SS, Argote CM, Dobrucali AM, Awayda MS, Orlando RC (2004) Dilated intercellular spaces and shunt permeability in nonerosive acid-damaged esophageal epithelium. Am J Gastroenterol 99:13–22CrossRefPubMed

54.

Calabrese C, Bortolotti M, Fabbri A, Areni A, Cenachi G, Scialpi C, Miglioli M, Di Febo G (2005) Reversibility of GERD Ultrastructural alterations and relief of symptoms after omeprazole treatment. Am J Gastroenterol 100:537–542CrossRefPubMed

55.

Villanacci V, Grigolato PG, Cestari R, Missale G, Cengia G, Klersy C, Rindi G (2001) Dilated intercellular spaces as markers of reflux disease: histology, semiquantitative score and morphometry upon light microscopy. Digestion 64:1–8CrossRefPubMed

56.

Locke GR, Talley NJ, Fett SL, Zinmeister AR, Melton LJ (1997) Prevalence and clinical spectrum of gastroesophageal reflux: a population-based study in Olmsted County, Minnesota. Gastroenterology 112:1448–1456CrossRefPubMed

57.

Gyorffy H, Holczbauer A, Nagy P, Szabo Z, Kupcsulik P, Paska C, Papp J, Schaff Z, Kiss A (2005) Claudin expression in Barrett’s esophagus and adenocarcinoma. Virchows Arch 447:961–968CrossRefPubMed

Titel: Transepithelial Leak in Barrett’s Esophagus
verfasst von: J. M. Mullin
M. C. Valenzano
S. Trembeth
P. D. Allegretti
J. J. Verrecchio
J. D. Schmidt
V. Jain
J. B. Meddings
G. Mercogliano
J. J. Thornton
Publikationsdatum: 01.12.2006
Verlag: Kluwer Academic Publishers-Plenum Publishers
Erschienen in: Digestive Diseases and Sciences / Ausgabe 12/2006
Print ISSN: 0163-2116
Elektronische ISSN: 1573-2568
DOI: https://doi.org/10.1007/s10620-006-9478-5

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

19.04.2024 | Vorhofflimmern | Nachrichten

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Transepithelial Leak in Barrett’s Esophagus

Abstract

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Parodontalbehandlung verbessert Prognose bei Katheterablation

Prostatakarzinom: EU initiiert neues Screeningkonzept

Blasenkarzinom – Biomarker statt Zytologie?

Antihypertensiva schützen auch alte Menschen noch vor Demenz

Update Innere Medizin

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 12/2006

Clostridium Difficile-Associated Pouchitis

The Effect of Insulin-like Growth Factor-I on Hepatocyte Apoptosis After Bile Duct Ligation in Rat

Expression of NF-κB in Helicobacter pylori Infection

An Unusual Case of a Ventral Richter’s Hernia at the Site of a Previous PEG Tube

Involvement of Cyclooxygenase-2–Prostaglandin E2 Pathway in Interleukin-8 Production in Gastric Cancer Cells

The Utility of Upper Endoscopy in Patients with Concomitant Upper Gastrointestinal Bleeding and Acute Myocardial Infarction

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Parodontalbehandlung verbessert Prognose bei Katheterablation

Prostatakarzinom: EU initiiert neues Screeningkonzept

Blasenkarzinom – Biomarker statt Zytologie?

Antihypertensiva schützen auch alte Menschen noch vor Demenz

Update Innere Medizin