nach oben

Digestive Diseases and Sciences

Erschienen in:

01.02.2008 | Original Paper

Application of Tissue Microarrays to Study the Influence of Dexamethasone on NF-κB Expression of Pancreas in Rat with Severe Acute Pancreatitis

verfasst von: Xi Ping Zhang, Ling Zhang, Hong Miao Xu, Yan Ping Xu, Qi Hui Cheng, Jian Mei Wang, Hai Ping Shen

Erschienen in: Digestive Diseases and Sciences | Ausgabe 2/2008

Einloggen, um Zugang zu erhalten

Abstract

To discuss the influence of dexamethasone on NF-κB expression of pancreas in rat with severe acute pancreatitis (SAP). Ninety rat SAP models were divided into the model group and dexamethasone treatment group with 45 rats in each group; another healthy 45 rats were selected to be the sham operation group. The groups were divided into the 3, 6 and 12 h group with 15 rats in each group. The survivals, pancreas pathological changes were observed 3, 6 and 12 h after operation. The changes in expression levels of NF-κB protein of pancreas tissue microarray were observed. The treatment group was significantly lower than the model group at 3 and 6 h (P < 0.05) and than the model group at 12 h in pancreas pathological scores (P < 0.01). The expression level of NF-κB protein of pancreas head of the treatment group was significantly less than that of the model group at 3 h (P < 0.01). The alleviation of pancreatic tissue injury by dexamethasone during SAP might be closely related to its role in inhibiting NF-κB expression and regulating cytokines. The advantages of tissue microarrays in pancreatitis pathological examination include time and energy savings, high efficiency and representative results.

Wang ZF, Pan CE, Liu SH (1999) Role of inflammatory mediators in pathogenesis of severe acute pancreatitis (in Chinese). Chin Crit Care Med 11(5):312–313

Wang ZF, Pan CE, Liu SH (1999) Study progress in pathogenesis of severe acute pancreatitis (in Chinese). Chin J Gen Surg 14(2):144–146

Chen F, Castranova V, Shi XL, Demers LM (1999) New insights in the role of nuclear factor-κB, a ubiquitous transcription factor in the initiation of disease. Clin Chem 45:7–17 [PMID: 11485895]PubMed

Pei HH, Yang ZA, Qin ZY (2001) NF-κB and acute pancreatitis (in Chinese). Chin Crit Care Med 13(4):248–249

Wang GQ, Chen YL (1997) Study progress on NF-κB. Adv Physiol (Chinese) 28(2):148–150

Zhang XP, Zhang L, Chen LJ, Cheng QH, Wang JM, Cai W, Shen HP, Cai J (2007) Influence of dexamethasone on inflammatory mediators and NFkappaB expression in multiple organs of rats with severe acute pancreatitis. World J Gastroenterol 13(4):548–556 [PMID: 17278220]PubMedCrossRef

Zhang XP, Zhang L, He JX, Zhang RP, Cheng QH, Zhou YF, Lu B (2007) Experimental study of therapeutic efficacy of Baicalin in rats with severe acute pancreatitis. World J Gastroenterol 13(5):717–724 [PMID: 17278194]PubMed

Norman J (1998) The role of cytokines in the pathogenesis of acute pancreatitis. Am J Surg 175:76–83 [PMID: 9445247]PubMedCrossRef

Neurath MF, Becker C, Barbulescu K (1998) Role of NF-kappaB in immune and inflammatory responses in the gut. Gut 43(6):856–860 [PMID: 9824616]PubMedCrossRef

10.

Dunn JA, Li C, Ha T, Kao RL, Browder W (1997) Therapeutic modification of nuclear factor kappa B binding activity and tumor necrosis factor-alpha gene expression during acute biliary pancreatitis. Am Surg 63(12):1036–1043; discussion 1043–1044. [PMID: 9393250]

11.

Gukovsky I, Gukovskaya AS, Blinman TA, Zaninovic V, Pandol SJ (1998) Early NF-κB activation is associated with hormone-induced pancreatitis. AM J physiol 275(6 Pt 1):G1402–G1414 [PMID: 9843778]PubMed

12.

Altavilla D, Famulari C, Passaniti M, Galeano M, Macri A, Seminara P, Minutoli L, Marini H, Calo M, Venuti FS, Esposito M, Squadrito F (2003) Attenuated cerulein-induced pancreatitis in nuclear factor-kappaB-deficient mice. Lab Invest 83(12):1723–1732 [PMID: 14691290]PubMedCrossRef

13.

Chen X, Ji B, Han B, Ernst SA, Simeone D, Logsdon CD (2002) NF-kappaB activation in pancreas induces pancreatic and systemic inflammatory response. Gastroenterology 122:448–457 [PMID: 11832459]PubMedCrossRef

14.

Gukovsky I, Gukovskaya AS, Blinman TA, Zaninovic V, Pandol SJ (1998) Early NF-kappa B activation is associated with hormone-induced pancreatitis. Am J Physiol 275:G1402–G1414 [PMID:984377]PubMed

15.

Bai XW, Sun B (2004) Severe acute pancreatitis and nuclear factor. J Harbin Medical University (Chinese) 38:488–489

16.

Pei HH, Yang ZA, Qin ZY, Feng YQ (2002) Significance of NF-κB expression in acute necrotic pancreatitis of rat (in Chinese). Chin J Gen Surg 17:752

17.

Pei HH, Yang ZA, Qin ZY, Feng YQ (2002) Significance of NF-κB expression in two experimental pancreatitis (in Chinese). Chin J Crit Care Med 22:72–73

18.

Stephenson HE Jr, Pfeffer RB, Saupol GM (1952) Acute hemorrhagic pancreatits: report of a case with cortson treatment. AMA Arch Surg 65(2):307–308 [PMID: 14943366]PubMed

19.

Zhu QF (1997) Clinical and pathologic analysis on 20 autopsy cases with acute necrotizing pancrititis (in Chinese). J Hepatopancreatobiliary Surg 3(4):30–31

20.

Guo LX, Luan MX (2002) 2 Cases of glucocorticoid-induced acute pancreatitis in children (in Chinese). J Clin Pediatr Surg 1(1):87–88

21.

Yue MX, Zhang GX, Li CL, Li XB, Zhang LC, Zhang SL, Xue L, Wang XM (1997) The effect of anisodaminum and dexamethasone on microcirculation in rabbit with multiple organ dysfunction syndrome (in Chinese). Microcirculation 7(3):10–11

22.

Liu JS, Wei XG, Fu J, Liu J, Yuan YZ, Wu YL (2003) Stady of the relationship among endothelin, nitric oxide,oxgen free radical and acute pancreatitis (in Chinese). J Chin Physician 5(1):28–29

23.

Chang CK, Llanes S, Schumer W (1997) Effect of dexamethasone on NF-κB activation, tumor necrosis factor formation, and glucose dyshomeostasis in septic rats (in Chinese). J Surg Res 72(2):141–145 [PMID: 9356235]PubMedCrossRef

24.

Meduri GU (1999) New rationale for glucocorticoid treatment in septic shock (in Chinese). J Chemother 11(6):541–545 [PMID: 10678798]PubMed

25.

Lanza L, Scudeletti M, Monaco E, Monetti M, Puppo F, Filaci G, Indiveri F (1999) Possible differences in the mechanism(s) of action of different glucocorticoid hormone compounds. Ann NY Acad Sci 876:193-197 [PMID: 10415609]PubMedCrossRef

26.

Moch H, Kononen T, Kallioniemi OP, Sauter G (2001) Tissue microarrays: what will they bring to molecular and anatomic pathology? Adv Anat Pathol 8(1):14–20 [PMID: 11152090]PubMedCrossRef

27.

Kononen J, Bubendorf L, Kallioniemi A, Barlund M, Schraml P, Leighton S, Torhorst J, Mihatsch MJ, Sauter G, Kallioniemi OP (1998) Tissue microarrays for high-throughput molecular profiling of tumor specimens. Nat Med 4(7):844–847 [PMID: 9662379]PubMedCrossRef

28.

Zhou XG, Zhang JS, Zhang XP, Zhang Y, Sandvej K, Stephen JHD (2002) Tissue chip (in Chinese). Chin J Pathol 31(2):70–71

29.

Li J, Liu L, Li Q (2003) Tissue microarrays and its application in medical study. Chin J Cancer Prev Treat 10(2):207–211

30.

Chen Q, Shi QL (2003) Tissue microarrays and its application in lymphoma study (in Chinese). Chin J Diagn Pathol 10(3):182–184

31.

Yan XW, Chen Q, Shi QL (2003) Application of tissue chip technique in pathology (in Chinese). J Med Postgrad 16(4):297–299, 303

32.

Wang Y, Chen MH (2004) Progress and application of tissue chip technique (in Chinese). J Shantou Univer Med Coll 17(3):185–186, 192

33.

Ekins R, Chu FW (1999) Microarrays: their origins and applications. Trends Biotechnol 17(6):217–218 [PMID: 10354556]PubMedCrossRef

34.

Wooster R (2000) Cancer classification with DNA microarrays in less more. Trends Genet 16(8):327–329 [PMID: 10904257]PubMedCrossRef

35.

Sallinen SL, Sallinen PK, Haapasalo HK, Helin HJ, Helen PT, Schraml P, Kallioniemi OP, Kononen J (2000) Identification of differentially expressed genes in human gliomas by DNA microarray and tissue chip techniques. Cancer Res 60(23):6617–6622 [PMID: 11118044]PubMed

36.

Barlund M, Monni O, Kononen J, Cornelison R, Torhorst J, Sauter G, Kallioniemi O-P, Kallioniemi A (2000) Multiple genes at 17q23 undergo amplification and overexpression in breast cancer. Cancer Res 60(19):5340–5344 [PMID: 11034067]PubMed

37.

Harris NL, Jaffe ES, Diebold J, Flandrin G, Muller-Hermelink HK, Vardiman J, Lister TA, Bloomfield CD (2000) The World Health Organization classification of neoplastic diseases of the haematopoitic and lymphoid tissues: report of the Clinical Advisory Committee Meeting, Airlie House, Virginia, November 1997. Histopathology 36(1):69–86PubMedCrossRef

38.

Gaiser T, Thorns C, Merz H, Noack F, Feller AC, Lange K (2002) Gene profiling in anaplastic large-cell lymphoma-derived cell lines with cDNA expression arrays. J Hematother Stem Cell Res 11(2):423–428 [PMID: 11983114]PubMedCrossRef

39.

Husson H, Carideo EG, Neuberg D, Schultze J, Munoz O, Marks PW, Donovan JW, Chillemi AC, O’Connell P, Freedman AS (2002) Gene expression profiling of follicular lymphoma and normal germinal center B cells using cDNA arrays. Blood 99(1):282–289 [PMID: 11756183]PubMedCrossRef

40.

Oka T, Yoshino T, Hayashi K, Ohara N, Nakanishi T, Yamaai Y, Hiraki A, Sogawa CA, Kondo E, Teramoto N, Takahashi K, Tsuchiyama J, Akagi T (2001) Reduction of hematopoietic cell-specific tyrosine phosphatase SHP-1 gene expression in natural killer cell lymphoma and various types of lymphomas/leukemias: combination analysis with cDNA expression array and tissue microarray. AM J Pathol 159(4):1495–1505 [PMID: 11583976]PubMed

41.

Sundram U, Kim Y, Mraz-Gernhard S, Hoppe R, Natkunam Y, Kohler S (2001) Analysis of MUM1/IRF4 protein expression using tissue microarrays and immunohistochemistry. Mod Pathol 14(7):686–694 [PMID: 15701085]CrossRef

42.

Florell SR, Coffin CM, Holden JA, Zimmermann JW, Gerwels JW, Summers BK, Jones DA, Leachman SA (2001) Preservation of RNA for functional genomic studies: a multidisciplinary tumor bank protocol. Mod Pathol 14(2):116–128 [PMID: 11235903]PubMedCrossRef

43.

Kipps TJ (2002) Advances in classification and treatment of indolent B-cell malignancies. Semin Oncol 29(1 Suppl 2):98–104 [PMID: 11842396]PubMedCrossRef

44.

Manley S, Mucci NR, De Marzo AM, Rubin MA (2001) Relational database structure to manage high-density tissue microarray data and images for pathology studies focusing on clinical outcome: the prostate specialized program of research excellence model. Am J Pathol 159(3):837–843 [PMID: 11549576]PubMed

45.

Bubendorf L, Kolmer M, Kononen J, Koivisto P, Mousses S, Chen Y, Mahlamaki E, Schraml P, Moch H, Willi N, Elkahloun AG, Pretlow TG, Gasser TC, Mihatsch MJ, Sauter G, Kallioniemi OP (1999) Hormone treatment failure in human prostate:analysis by complementary DNA and tissue microarrays. J Natl cancer Inst 91(20):1758–1764 [PMID: 10528027]PubMedCrossRef

46.

Barlund M, Forozan F, Kononen J, Bubendorf L, Chen Y, Bittner ML, Torhorst J, Haas P, Bucher C, Sauter G, Kallioniemi OP, Kallioniemi A (2000) Detecting activation of ribosomal protein S6 kinase by complementary DNA and tissue microarray analysis. J Natl Cancer Inst 92(15):1252–1259 [PMID: 10922410]PubMedCrossRef

47.

Parker RL, Huntsman DG, Lesack DW, Cupples JB, Grant DR, Akbari M, Gilks CB (2002) Assessment of interlaboratory variation in the immunohistochemical determanation of estrogen receptor status using a breast cancer tissue microaray. Am J Clin Pathol 117(5):723–728 [PMID: 12090420]PubMedCrossRef

48.

Dong R, Wang ZF, Lv Y, Ma QJ (2005) Treatment of severe acute pancreatitis with large dosage of dexamethasone in the earlier time (in Chinese). J Hepatobiliary Surg 13(1):58–60

49.

Wu SS (1992) The prevention and treatment in multiple organ dysfunction syndrome following septic shock (in Chinese). Chin J Pract Surg 9(12):472–473

50.

Liang ZY (2001) The treatment effect of early using dexamethasone in severe acute pancreatitis (in Chinese). Chin J Crit Care Med 21(12):726–727

Titel: Application of Tissue Microarrays to Study the Influence of Dexamethasone on NF-κB Expression of Pancreas in Rat with Severe Acute Pancreatitis
verfasst von: Xi Ping Zhang
Ling Zhang
Hong Miao Xu
Yan Ping Xu
Qi Hui Cheng
Jian Mei Wang
Hai Ping Shen
Publikationsdatum: 01.02.2008
Verlag: Springer US
Erschienen in: Digestive Diseases and Sciences / Ausgabe 2/2008
Print ISSN: 0163-2116
Elektronische ISSN: 1573-2568
DOI: https://doi.org/10.1007/s10620-007-9867-4

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

^{Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag}

Kostenlos registrieren

Neu im Fachgebiet Innere Medizin

23.04.2024 | Ablationstherapie | Nachrichten

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.

Newsletter bestellen

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Application of Tissue Microarrays to Study the Influence of Dexamethasone on NF-κB Expression of Pancreas in Rat with Severe Acute Pancreatitis

Abstract

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Wie erfolgreich ist eine Re-Ablation nach Rezidiv?

Europäische Ernährungsgewohnheiten: Das sind die häufigsten Fehler

Hinter dieser Appendizitis steckte ein Erreger

Demenzkranke durch Antipsychotika vielfach gefährdet

Update Innere Medizin

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 2/2008

Does Mutation of Hepatitis A Virus Exist in North India?

Subtotal Splenectomy and Central Splenorenal Shunt for Treatment of Bleeding from Roux En Y Jejunal Loop Varices Secondary to Portal Hypertension

HLA DRB1*13 as a Risk Factor for Type 1 Autoimmune Hepatitis in North American Patients

Association between Proton Pump Inhibitor Use and Spontaneous Bacterial Peritonitis

Clinical Outcomes of Patients with Liver Cirrhosis Who Underwent Curative Surgery for Gastric Cancer: A Retrospective Multi-center Study

Ethnic Differences in Hepatocellular Carcinoma: Implications for Liver Transplantation

Leitlinien kompakt für die Innere Medizin

Neu im Fachgebiet Innere Medizin

Wie erfolgreich ist eine Re-Ablation nach Rezidiv?

Europäische Ernährungsgewohnheiten: Das sind die häufigsten Fehler

Hinter dieser Appendizitis steckte ein Erreger

Demenzkranke durch Antipsychotika vielfach gefährdet

Update Innere Medizin