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Digestive Diseases and Sciences

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01.11.2011 | Original Article

Effects of Bifidobacterium infantis 35624 on Post-Inflammatory Visceral Hypersensitivity in the Rat

verfasst von: Anthony C. Johnson, Beverley Greenwood-Van Meerveld, John McRorie

Erschienen in: Digestive Diseases and Sciences | Ausgabe 11/2011

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Abstract

Background

Irritable bowel syndrome patients have abnormal visceral perception. Probiotic organisms may produce beneficial effects in these patients by reducing visceral hypersensitivity.

Aim

To investigate the effects of the probiotic organism, Bifidobacterium infantis 35624, on post-inflammatory visceral hypersensitivity in rats.

Methods

Colitis was induced using intracolonic administration of trinitrobenzenesulfonic acid; control rats received saline (day 0). Myeloperoxidase (MPO) levels and colonic damage scores were determined. From days 15–29, rats (n = 10/group) rats were orally dosed with 2 ml of B. infantis ≥ 10⁸ colony-forming units/ml or vehicle (MRS broth). A second series of rats (n = 10/group) was dosed in the same manner from days 15–59. The level of colonic stimulation during colorectal distension (CRD) was determined by recording a visceromotor response (VMR) to CRD at 30 mmHg pre- and post-treatment. Post-treatment samples of colonic tissue were weighed, graded for morphologic damage, and assayed for MPO levels.

Results

All rats were hypersensitive at day 15. On day 30, hypersensitivity to colorectal distension remained in the vehicle group, but was significantly reduced in the B. infantis group (mean VMR/10 min: vehicle = 15.4 ± 1.0 vs. B. infantis = 7.6 ± 1.0, p < 0.001). A similar, significant effect was observed at day 60. On both day 30 and day 60, tissue weight, colonic damage scores, and MPO levels resembled those of control animals.

Conclusions

Oral administration of Bifidobacterium infantis 35624 normalized sensitivity to colorectal distension in a rat model of post-inflammatory colonic hypersensitivity.

Mayer EA, Gebhart GF. Basic and clinical aspects of visceral hyperalgesia. Gastroenterology. 1994;107:271–293.PubMed

Ritchie J. Pain from distension of the pelvic colon by inflating a balloon in the irritable colon syndrome. Gut. 1973;14:125–132.PubMedCrossRef

Whitehead WE, Holtkotter B, Enck P, et al. Tolerance for rectosigmoid distention in irritable bowel syndrome. Gastroenterology. 1990;98:1187–1192.PubMed

Chaudhary NA, Truelove SC. Human colonic motility. A comparative study of normal subjects, patients with ulcerative colitis, and patients with the irritable colon syndrome. Gastroenterology. 1968;54:777–778.PubMed

Collins SM. Stress and the gastrointestinal tract IV. Modulation of intestinal inflammation by stress: basic mechanisms and clinical relevance. Am J Physiol Gastrointest Liver Physiol. 2000;280:G315–G318.

Sartor RB, Bond TM, Schwab JH. Systemic uptake and intestinal inflammatory effects of luminal bacterial cell wall polymers in rats with acute colonic injury. Infect Immun. 1988;56:2101–2108.PubMed

Shorter RG, Tomasi TB, Huizenga KA, Spencer RJ, Stobo JD. The immunology of chronic ulcerative colitis and Crohn’s disease. Ann N Y Acad Sci. 1976;278:586–591.PubMedCrossRef

Gionchetti P, Rizzello F, Venturi A, Campieri M. Probiotics in infective diarrhoea and inflammatory bowel disease. J Gastoenterol Hepatol. 2000;15:489–493.CrossRef

Rolfe RD. The role of probiotic cultures in the control of gastrointestinal health. J Nutr. 2000;130:396S–402S.PubMed

10.

O’Mahony L, McCarthy J, Kelly P, et al. Lactobacillus and Bifidobacterium in irritable bowel syndrome: symptom responses and relationship to cytokine profiles. Gastroenterology. 2005;128:541–551.PubMedCrossRef

11.

Whorwell PJ, Altringer L, Morel J, et al. Efficacy of an encapsulated probiotic Bifidobacterium infantis 35624 in women with irritable bowel syndrome. Am J Gastroenterol. 2006;101:1581–1590.PubMedCrossRef

12.

Lacheze C, Coelho A-M, Fioramonti J, Bueno L. Influence of trimebutine on inflammation- and stress-induced hyperalgesia to rectal distension in rates. J Pharm Pharmacol. 1998;50:921–928.PubMedCrossRef

13.

Goldhill J, Pichat P, Roome N, Angel I, Arbilla S. Effect of mizolastine on visceral sensory afferent sensitivity and inflammation during experimental colitis. Arzneimittel-Forschung. 1998;48:170–184.

14.

Gibson MS, Greenwood-Van Meerveld B. An acute inflammatory insult induces long term colonic hypersensitivity. Am J Gastroenterol. 2000;95:2534. [abstract].CrossRef

15.

Diop L, Raymond F, Fargeau H, Petoux F, Chovet M, Doherty AM. Pregabaline (CI-1008) inhibits the trinitrobenzene sulfonic acid acid-induced chronic colonic allodynia in the rat. J Pharmacol Exp Ther. 2002;302:1013–1022.PubMedCrossRef

16.

Greenwood-Van Meerveld B, Johnson AC, Foreman RD, Linderoth B. Spinal cord stimulation attenuates visceromotor reflexes in a rat model of post-inflammatory colonic hypersensitivity. Auton Neurosci. 2005;122:69–76.PubMedCrossRef

17.

Morris GP, Beck PL, Herridge MS, Depew W, Szewczuk MR, Wallace JL. Hapten-induced model of chronic inflammation and ulceration in the rat colon. Gastroenterology. 1989;96:795–803.PubMed

18.

Krawisz JE, Sharon P, Stenson WF. Quantitative assay for acute intestinal inflammation based on myeloperoxidase activity. Assessment of inflammation in rat and hamster models. Gastroenterology. 1984;87:1344–1350.PubMed

19.

McKernan DP, Fitzgerald P, Dinan TG, Cryan JF. The probiotic Bifidobacterium infantis 35624 displays visceral antinociceptive effects in the rat. Neurogastroenterol Motil. 2010;22:1029–1035. e268.PubMedCrossRef

20.

Verdú EF, Bercik P, Verma-Gandhu M, et al. Specific probiotic therapy attenuates antibiotic induced visceral hypersensitivity in mice. Gut. 2006;55:182–190.PubMedCrossRef

21.

Ait-Belgnaoui A, Han W, Lamine F, et al. Lactobacillus farciminis treatment suppresses stress induced visceral hypersensitivity: a possible action through interaction with epithelial cell cytoskeleton contraction. Gut. 2006;55:1090–1094.PubMedCrossRef

22.

Kamiya T, Wang L, Forsythe P, et al. Inhibitory effects of Lactobacillus reuteri on visceral pain induced by colorectal distension in Sprague-Dawley rats. Gut. 2006;55:191–196.PubMedCrossRef

23.

Collins SM, Bercik P. The relationship between intestinal microbiota and the central nervous system in normal gastrointestinal function and disease. Gastroenterology. 2009;136:2003–2014.PubMedCrossRef

24.

Sibartie S, O’Hara AM, Ryan J, et al. Modulation of pathogen-induced CCL20 secretion from HT-29 human intestinal epithelial cells by commensal bacteria. BMC Immunol. 2009;10:54.PubMedCrossRef

25.

O’Mahony L, O’Callaghan L, McCarthy J, et al. Differential cytokine response from dendritic cells to commensal and pathogenic bacteria in different lymphoid compartments in humans. Am J Physiol Gastrointest Liver Physiol. 2006;290:G839–G845.PubMedCrossRef

26.

Davies JM, Sheil B, Shanahan F. Bacterial signalling overrides cytokine signalling and modifies dendritic cell differentiation. Immunology. 2009;128:e805–e815.PubMedCrossRef

27.

Kassinen A, Krogius-Kurikka L, Mäkivuokko H, et al. The fecal microbiota of irritable bowel syndrome patients differs significantly from that of healthy subjects. Gastroenterology. 2007;133:24–33.PubMedCrossRef

28.

Tana C, Umesaki Y, Imaoka A, Handa T, Kanazawa M, Fukudo S. Altered profiles of intestinal microbiota and organic acids may be the origin of symptoms in irritable bowel syndrome. Neurogastroenterol Motil. 2010;22:512–519. e114-e115.PubMed

29.

García-Lafuente A, Antolín M, Guarner F, et al. Incrimination of anaerobic bacteria in the induction of experimental colitis. Am J Physiol. 1997;272:G10–G15.PubMed

30.

Hernández GA, Appleyard CB. Bacterial load in animal models of acute and chronic ‘reactivated’ colitis. Digestion. 2003;67:161–169.PubMedCrossRef

31.

Spiller R, Garsed K. Postinfectious irritable bowel syndrome. Gastroenterology. 2009;136:1979–1988.PubMedCrossRef

32.

Barbara G, Cremon C, Pallotti F, De Giorgio R, Stanghellini V, Corinaldesi R. Postinfectious irritable bowel syndrome. J Pediatr Gastroenterol Nutr. 2009;48:S95–S97.PubMedCrossRef

33.

Szepes Z, Kiss J, Molnár T, Lamarque D, Jancsó G, László F. Capsaicin-sensitive mechanisms in the modulation of rat colonic vascular permeability under physiological and pathological conditions. J Physiol Paris. 1997;91:123–126.PubMedCrossRef

34.

Mazelin L, Theodorou V, More J, Fioramonti J, Bueno L. Protective role of vagal afferents in experimentally-induced colitis in rats. J Auton Nerv Syst. 1998;73:38–45.PubMedCrossRef

35.

Stein J, Ries J, Barrett KE. Disruption of intestinal barrier function associated with experimental colitis: possible role of mast cells. Am J Physiol. 1998;274:G203–G209.PubMed

36.

Natah SS, Mouihate A, Pittman QJ, Sharkey KA. Disruption of the blood-brain barrier during TNBS colitis. Neurogastroenterol Motil. 2005;17:433–446.PubMedCrossRef

37.

Greenwood-Van Meerveld B, Johnson AC, Schulkin J, Myers DA. Long-term expression of corticotropin-releasing factor (CRF) in the paraventricular nucleus of the hypothalamus in response to an acute colonic inflammation. Brain Res. 2006;1071:91–96.PubMedCrossRef

38.

Lamine F, Fioramonti J, Bueno L, et al. Nitric oxide released by Lactobacillus farciminis improves TNBS-induced colitis in rats. Scand J Gastroenterol. 2004;39:37–45.PubMedCrossRef

39.

Lamine F, Eutamène H, Fioramonti J, Buéno L, Théodorou V. Colonic responses to Lactobacillus farciminis treatment in trinitrobenzene sulphonic acid-induced colitis in rats. Scand J Gastroenterol. 2004;39:1250–1258.PubMedCrossRef

40.

Kennedy RJ, Hoper M, Deodhar K, Kirk SJ, Gardiner KR. Probiotic therapy fails to improve gut permeability in a hapten model of colitis. Scand J Gastroenterol. 2000;35:1266–1271.PubMedCrossRef

41.

Sommerfield D, O’Brien N, Kiely B, et al. Influence of Bifidobacterium infantis feeding on secretagogue response and gut barrier function in rats recovering from colitis. Gastroenterology 2003;124:A311. M1113 [abstract].

42.

Ewaschuk JB, Diaz H, Meddings L, et al. Secreted bioactive factors from Bifidobacterium infantis enhance epithelial cell barrier function. Am J Physiol Gastrointest Liver Physiol. 2008;295:G1025–G1034.PubMedCrossRef

43.

Vergnolle N. Postinflammatory visceral sensitivity and pain mechanisms. Neurogastroenterol Motil. 2008;20:73–80.PubMedCrossRef

44.

Hughes PA, Brierley SM, Blackshaw LA. Post-inflammatory modification of colonic afferent mechanosensitivity. Clin Exp Pharmacol Physiol. 2009;36:1034–1040.PubMedCrossRef

45.

Zhou Q, Price DD, Caudle RM, Verne GN. Spinal NMDA NR1 subunit expression following transient TNBS colitis. Brain Res. 2009;1279:109–120.PubMedCrossRef

46.

Zhou Q, Caudle RM, Price DD, Del Valle-Pinero AY, Verne GN. Selective up-regulation of NMDA-NR1 receptor expression in myenteric plexus after TNBS induced colitis in rats. Mol Pain. 2006;2:3.PubMedCrossRef

47.

Keating C, Beyak M, Foley S, et al. Afferent hypersensitivity in a mouse model of post-inflammatory gut dysfunction: role of altered serotonin metabolism. J Physiol. 2008;586:4517–4530.PubMedCrossRef

48.

Choi YD, Sung TS, Kim HJ, La JH, Kim TW, Yang IS. Increased 5-hydroxytryptamine mediates post-inflammatory visceral hypersensitivity via the 5-hydroxytryptamine 3 receptor in rats. Dig Dis Sci. 2008;53:2909–2916.PubMedCrossRef

49.

Desbonnet L, Garrett L, Clarke G, Bienenstock J, Dinan TG. The probiotic Bifidobacteria infantis: an assessment of potential antidepressant properties in the rat. J Psychiatr Res. 2008;43:164–174.PubMedCrossRef

50.

Desbonnet L, Garrett L, Clarke G, Kiely B, Cryan JF, Dinan TG. Effects of the probiotic Bifidobacterium infantis in the maternal separation model of depression. Neuroscience. 2010;170:1179–1188.PubMedCrossRef

51.

van der Kleij H, O’Mahony C, Shanahan F, O’Mahony L, Bienenstock J. Protective effects of Lactobacillus reuteri and Bifidobacterium infantis in murine models for colitis do not involve the vagus nerve. Am J Physiol Regul Integr Comp Physiol. 2008;295:R1131–R1137.PubMedCrossRef

52.

Diop L, Rivière PJ, Pascaud X, Dassaud M, Junien JL. Role of vagal afferents in the antinociception produced by morphine and U-50, 488H in the colonic pain reflex in rats. Eur J Pharmacol. 1994;257:181–187.PubMedCrossRef

53.

Gschossmann JM, Mayer EA, Miller JC, Raybould HE. Subdiaphragmatic vagal afferent innervation in activation of an opioidergic antinociceptive system in response to colorectal distension in rats. Neurogastroenterol Motil. 2002;14:403–408.PubMedCrossRef

Titel: Effects of Bifidobacterium infantis 35624 on Post-Inflammatory Visceral Hypersensitivity in the Rat
verfasst von: Anthony C. Johnson
Beverley Greenwood-Van Meerveld
John McRorie
Publikationsdatum: 01.11.2011
Verlag: Springer US
Erschienen in: Digestive Diseases and Sciences / Ausgabe 11/2011
Print ISSN: 0163-2116
Elektronische ISSN: 1573-2568
DOI: https://doi.org/10.1007/s10620-011-1730-y

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Effects of Bifidobacterium infantis 35624 on Post-Inflammatory Visceral Hypersensitivity in the Rat

Abstract

Background

Aim

Methods

Results

Conclusions

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Abstract

Background

Aim

Methods

Results

Conclusions

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