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Erschienen in: Documenta Ophthalmologica 3/2018

05.04.2018 | Original Research Article

Visual evoked potentials can be reliably recorded using noninvasive epidermal electrodes in the anesthetized rat

verfasst von: Roberto Santangelo, Valerio Castoldi, Raffaele D’Isa, Silvia Marenna, Su-Chun Huang, Marco Cursi, Giancarlo Comi, Letizia Leocani

Erschienen in: Documenta Ophthalmologica | Ausgabe 3/2018

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Abstract

Purpose

Visual evoked potentials (VEPs) are a powerful tool to evaluate nervous conduction along the visual pathways, both in humans and in animal models. Traditionally, epidural screw electrodes are used to record VEPs in preclinical research. Here we tested the feasibility in the preclinical setting of the same noninvasive technique used for clinical VEP acquisition, by using epidermal cup electrodes with no surgical procedures.

Methods

Monocular flash VEPs were recorded bilaterally under sevoflurane anesthesia once a week for 6 weeks in 14 dark Agouti rats, 7 with implanted epidural screws and 7 with epidermal 6 mm Ø Ag/AgCl cups.

Results

VEP traces obtained with the two techniques were morphologically comparable. There were no significant differences in latency of the main visual component between screw-recorded VEPs (sVEPs) and cup-recorded VEPs (cVEPs). Amplitude values with epidermal cups were significantly lower than those with epidural screws. Both techniques provided latencies and amplitudes which were stable over time. Furthermore, with regard to latency both methods ensured highly repeatable measurements over time, with epidermal cups even providing slightly better results. On the other hand, considering amplitudes, cVEPs and sVEPs provided fairly acceptable repeatability.

Conclusions

Epidermal cup electrodes can provide comparable results to those obtained with the “gold standard” epidural screws, while representing a simpler and less invasive technique to test nervous conduction along the visual pathways in the preclinical setting.
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Metadaten
Titel
Visual evoked potentials can be reliably recorded using noninvasive epidermal electrodes in the anesthetized rat
verfasst von
Roberto Santangelo
Valerio Castoldi
Raffaele D’Isa
Silvia Marenna
Su-Chun Huang
Marco Cursi
Giancarlo Comi
Letizia Leocani
Publikationsdatum
05.04.2018
Verlag
Springer Berlin Heidelberg
Erschienen in
Documenta Ophthalmologica / Ausgabe 3/2018
Print ISSN: 0012-4486
Elektronische ISSN: 1573-2622
DOI
https://doi.org/10.1007/s10633-018-9630-5

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