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Tomato chlorotic dwarf viroid in the ornamental plant Vinca minor and its transmission through tomato seed

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Abstract

Two novel aspects of Tomato chlorotic dwarf viroid (TCDVd) are reported, namely that TCDVd was detected in symptomless plants of Vinca minor, a trailing ground cover surviving at subzero temperatures (−12°C); and that TCDVd was seed-borne in tomato and detected in high percentages in tomato seeds and seedlings. Soaking seeds in a low concentration of sodium hypochlorite did not eliminate the viroid. The sequence analysis showed that the TCDVd isolate consists of 360 nucleotides and has sequence identity between 96% to 99% with isolates of TCDVd from other hosts.

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References

  • Benson, A. P., & Singh, R. P. (1964). Seed transmission of Potato spindle tuber virus in tomato. American Potato Journal, 41, 294.

    Google Scholar 

  • Bostan, H., Nie, X., & Singh, R. P. (2004). An RT-PCR primer pair for the detection of Pospiviroid and its application in surveying ornamental plants for viroids. Journal of Virological Methods, 116, 189–193.

    Article  PubMed  CAS  Google Scholar 

  • Cammack, R. H., & Harris, P. S. (1973). Potato spindle tuber in Commonwealth Potato Collection. EPPO Bulletin, 3, 117–118.

    Article  Google Scholar 

  • Diener, T. O. (1971). Potato spindle tuber ‘virus’ IV. A replicating low molecular weight RNA. Virology, 45, 411–428.

    Article  PubMed  CAS  Google Scholar 

  • Fernow, K. H., Peterson, L. C., & Plaisted, R. L. (1970). Spindle tuber virus in seeds and pollen of infected potato plants. American Potato Journal, 47, 75–80.

    Article  Google Scholar 

  • Flores, R., Randles, J. W., Owens, R. A., Bar-Joseph, M., & Diener, T. O. (2005). Viroids. In C. M. Fauquet, M. A. Mayo, J. Maniloff, U. Desselberger, & A. L. Ball (Eds.), Virus taxonomy, eighth report of the International Committee on Taxonomy of Viruses (pp. 1145–1159). London, UK: Elsevier.

    Google Scholar 

  • Grasmick, M. E., & Slack, S. A. (1986). Effect of Potato spindle tuber viroid on sexual reproduction and viroid transmission in true potato seed. Canadian Journal of Botany, 64, 336–340.

    Article  Google Scholar 

  • James, T., Mulholland, V., Jeffries, C., & Chard, J. (2007). First report of Tomato chlorotic dwarf viroid infecting commercial Petunia stocks in the United Kingdom. New Disease Reports, 15 (Feb-July). Retrieved from http://www.bspp.org.uk/ndr/july2007/2007–48.asp.

  • Keese, P., & Symons, R. H. (1985). Domains in viroids: evidence of intermolecular RNA rearrangement and their contribution to viroid evolution. Proceedings of the National Academy of Sciences, USA, 82, 4582–4586.

    Article  CAS  Google Scholar 

  • Lizàrraga, R. E., Salazar, L. F., Roca, W. M., & Schilde-Rentschler, L. (1980). Elimination of potato spindle tuber by low temperature and meristem culture. Phytopathology, 70, 754–755.

    Article  Google Scholar 

  • Matsushita, Y., Kanda, A., Usugi, T., & Tsuda, S. (2008). First report of a Tomato chlorotic dwarf viroid disease on tomato plants in Japan. Journal of General Plant Pathology, 74, 182–184.

    Article  CAS  Google Scholar 

  • Paduch-Cichal, E., & Kryczynski, S. (1987). A low temperature therapy and meristem- tip culture for eliminating four viroids from infected plants. Journal of Phytopathology, 118, 341–346.

    Article  Google Scholar 

  • Sänger, H. L., Klotz, G., Riesner, D., Gross, H. J., & Kleinschmidt, A. K. (1976). Viroids are single-stranded covalently closed circular RNA molecules existing as highly base-paired rod-like structures. Proceedings of the National Academy of Sciences, USA, 73, 3852–3856.

    Article  Google Scholar 

  • Sänger, H. L., & Ramm, K. (1975). Radioactive labelling of viroid-RNA. In R. Markham, D. R. Davies, D. A. Hopwood, & R. W. Horne (Eds.), Modification of the information content of plant cells (pp. 229–252). Amsterdam: North Holland.

    Google Scholar 

  • Singh, R. P. (1970). Seed transmission of Potato spindle tuber virus in tomato and potato. American Potato Journal, 47, 225–227.

    Article  Google Scholar 

  • Singh, R. P. (1983). Viroids and their potential danger to potatoes in hot climates. Canadian Plant Disease Survey, 63, 13–18.

    Google Scholar 

  • Singh, R. P., & Boucher, A. (1987). Electrophoretic separation of a severe from mild strains of Potato spindle tuber viroid. Phytopathology, 77, 1588–1591.

    Article  Google Scholar 

  • Singh, R. P., Boucher, A., & Seabrook, J. E. A. (1988). Detection of mild strains of Potato spindle tuber viroid from single true potato seed by return electrophoresis. Phytopathology, 78, 663–667.

    Article  Google Scholar 

  • Singh, R. P., Boucher, A., & Somerville, T. H. (1989). Evaluation of chemicals for disinfection of laboratory equipment exposed to Potato spindle tuber viroid. American Potato Journal, 66, 239–246.

    Article  CAS  Google Scholar 

  • Singh, R. P., Boucher, A., & Somerville, T. H. (1992). Detection of Potato spindle tuber viroid in the pollen and various parts of potato pollinated with viroid-infected pollen. Plant Disease, 76, 951–953.

    Google Scholar 

  • Singh, R. P., Boucher, A., & Wang, R. G. (1991). Detection, distribution and long-term persistence of Potato spindle tuber viroid in true potato seed from Heiongjiang, China. American Potato Journal, 68, 65–74.

    Article  CAS  Google Scholar 

  • Singh, R. P., & Clark, M. C. (1971). Infectious low-molecular weight ribonucleic acid from tomato. Biochemical Biophysical Research Communications, 44, 1077–1083.

    Article  CAS  Google Scholar 

  • Singh, R. P., Dilworth, A. D., Baranwal, V. K., & Gupta, K. N. (2006b). Detection of Citrus exocortis viroid, Iresine viroid, and Tomato chlorotic dwarf viroid in new ornamental host plants in India. Plant Disease, 90, 1457.

    Article  Google Scholar 

  • Singh, R. P., Dilworth, A. D., Singh, M., & Babcock, K. M. (2006a). An alkaline solution simplifies nucleic acid preparation for RT-PCR and infectivity assays of viroids from crude sap and spotted membrane. Journal of Virological Methods, 132, 204–211.

    Article  PubMed  CAS  Google Scholar 

  • Singh, R. P., & Finnie, R. E. (1973). Seed transmission of Potato spindle tuber metavirus through the ovule of Scopolia sinensis. Canadian Plant Disease Survey, 53, 153–154.

    Google Scholar 

  • Singh, R. P., Nie, X., & Singh, M. (1999). Tomato chlorotic dwarf viroid an evolutionary link in the origin of Pospiviroids. Journal of General Virology, 80, 2823–2828.

    PubMed  CAS  Google Scholar 

  • Singh, R. P., & Teixeria da Silva, J. A. (2006). Ornamental plants: Silent carrier of evolving viroids. In J. A. Teixeria da Silva (Ed.), Floriculture, ornamental and plant biotechnology, Vol. III (pp. 531–539). London, UK: Global Science Books.

    Google Scholar 

  • Verhoeven, J. T. J., Jansen, C. C. C., Werkman, A. W., & Roenhorst, J. W. (2007). First report of Tomato chlorotic dwarf viroid in Petunia hybrida from the United States of America. Plant Disease, 91, 324.

    Article  Google Scholar 

  • Verhoeven, J. Th. J., Jansen, C. C. C., Willemen, T. M., Kox, L. F. F., Owens, R. A., & Roenhorst, J. W. (2004). Natural infection of tomato by Citrus exocortis viroid, Columnea latent viroid, Potato spindle tuber viroid and Tomato chlorotic dwarf viroid. European Jornal of Plant Patholology, 110, 823–831.

    Article  CAS  Google Scholar 

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Acknowledgements

We gratefully acknowledge the editorial critique by Dr. A. Singh (AgraPoint International Inc, Nova Scotia, Canada) and the technical assistance of Sarah Loftus.

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Authors and Affiliations

  1. Potato Research Centre, Agriculture and Agri-Food Canada, 850 Lincoln Road, P.O. Box 20280, Fredericton, NB, E3B 4Z7, USA

    Rudra P. Singh & Andrea D. Dilworth

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  1. Rudra P. Singh
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  2. Andrea D. Dilworth
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Correspondence to Rudra P. Singh.

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Singh, R.P., Dilworth, A.D. Tomato chlorotic dwarf viroid in the ornamental plant Vinca minor and its transmission through tomato seed. Eur J Plant Pathol 123, 111–116 (2009). https://doi.org/10.1007/s10658-008-9344-8

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  • DOI: https://doi.org/10.1007/s10658-008-9344-8

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