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Tomato necrotic ring virus (TNRV), a recently described tospovirus species infecting tomato and pepper in Thailand

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Abstract

Two tospovirus isolates collected from tomato and bell pepper in Thailand were studied. The isolates induced severe necrotic mottling and/or necrotic spots and rings on the leaves and fruits of the respective plants as confirmed by back-inoculation. A polyclonal antiserum raised against its nucleocapsid (N) protein reacted only with an extract from plants infected with the homologous virus. Analysis of the nucleocapsid (N) gene sequence and its deduced amino acid sequence (Mw ∼31 kDa) showed 99% amino acid sequence homology with that of Tomato necrotic ring virus (TNRV). The nucleotide sequence of the 5΄ untranslated region and intergenic region flanking the N gene revealed typical features of the S RNA segment of tospoviruses. Mechanical inoculation of the virus on some plant species showed that most of the tested solanaceous species were susceptible to this virus. The biological, serological and molecular data presented here indicate that both isolates are identical to TNRV, a recently described tospovirus species in Thailand.

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Acknowledgments

This research was financially supported by a fellowship to the second and third authors by the East West Seed Company in Thailand and the Philippines, respectively.

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Authors and Affiliations

  1. Laboratory of Virology, Department of Plant Sciences, Wageningen University, P.O. Box 629, 6700 AP, Wageningen, the Netherlands

    Afshin Hassani-Mehraban, Richard Kormelink & Dick Peters

  2. Plant Pathology Department, Hortigenetics Research, East–West Seed Company, Chiang Mai, Thailand

    Sirirat Cheewachaiwit

  3. Plant Pathology Department, Research and Development, East–West Seed Company, Bulacan, the Philippines

    Cherry Relevante

Authors
  1. Afshin Hassani-Mehraban
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  2. Sirirat Cheewachaiwit
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  3. Cherry Relevante
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  4. Dick Peters
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Correspondence to Afshin Hassani-Mehraban.

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Hassani-Mehraban, A., Cheewachaiwit, S., Relevante, C. et al. Tomato necrotic ring virus (TNRV), a recently described tospovirus species infecting tomato and pepper in Thailand. Eur J Plant Pathol 130, 449–456 (2011). https://doi.org/10.1007/s10658-011-9771-9

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  • DOI: https://doi.org/10.1007/s10658-011-9771-9

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