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Expression of the proliferation-associated nuclear protein MIB-1 and its relationship with microvascular density in bone marrow biopsies of patients with myelodysplastic syndromes

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Abstract

The myelodysplastic syndromes (MDS) are a group of disorders characterized by dysplastic hemopoiesis and an increased risk of leukemic transformation. The process of angiogenesis has been implicated in the pathogenesis and evolution of MDS. In this study the proliferative activity and extent of angiogenesis was examined in bone marrow samples from 54 patients with MDS in relation to clinicopathologic features. Cellular proliferation and microvascular density (MVD) were examined immunohistochemically, using the monoclonal antibody MIB-1 (Ki-67) and an anti-CD34 monoclonal antibody respectively. Serum concentrations of interleukin-6 (IL-6) were measured by ELISA. The results showed that the MIB-1 Labeling Index (MIB-1 LI), MVD and IL-6 increased significantly with advancing severity of disease. Among the MDS-FAB subtypes, MIB-1 LI, MVD and IL-6 were significantly higher in RAEB-t, RAEB and CMML in comparison to RA and RARS (p < 0.0001 in all cases). Similarly, MIB-1 and MVD were increased in patients with score 3 in comparison to scores 0 and 1 in the IPSS system (p < 0.05). All parameters studied were significantly higher in patients versus controls. We conclude that cellular proliferative activity and angiogenesis are associated with disease progression in MDS patients.

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Authors and Affiliations

  1. Department of Haematology, Medical school of Crete, University Hospital of Heraklion, Crete

    Michael G Alexandrakis, George Katrinakis & George Tsirakis

  2. Department of Pathology, Medical School of Crete, University Hospital of Heraklion, Crete

    Constantina Dambaki & Efstathios N Stathopoulos

  3. Haematology Unit, 3rd Department of Internal Medicine, Medical School of Athens, Sotiria Hospital, Athens

    Freda H Passam

  4. Haematology Department, Medical School of Thessalia, University Hospital of Larisa, Thessalia

    Despina S Kyriakou

  5. Blood Transfusion Department, General Hospital of Rhodes, Greece

    John Konsolas

Authors
  1. Michael G Alexandrakis
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  2. Freda H Passam
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  3. Despina S Kyriakou
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  4. Constantina Dambaki
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  5. George Katrinakis
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  6. George Tsirakis
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  7. John Konsolas
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  8. Efstathios N Stathopoulos
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Correspondence to Michael G Alexandrakis.

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Alexandrakis, M.G., Passam, F.H., Kyriakou, D.S. et al. Expression of the proliferation-associated nuclear protein MIB-1 and its relationship with microvascular density in bone marrow biopsies of patients with myelodysplastic syndromes. J Mol Hist 35, 857–863 (2004). https://doi.org/10.1007/s10735-004-2341-0

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