Abstract
Signals from the T cell immunoglobulin and mucin-domain (TIM)-containing molecules have been demonstrated to be involved in regulating the progress of carcinoma. However, the expression and anatomical distribution of TIMs in Langerhans cell sarcoma (LCS), which is a rare malignancy derived from dendritic cells of the epidermis, has yet to be determined. In this study, the expression of TIM-1, TIM-3 and TIM-4 in LCS samples were detected by immunohistochemistry. Our results showed that these three molecules were found in LCS sections. At the cellular level, these molecules were found on the cell membrane and in the cytoplasm. Immunofluorescence double-staining demonstrated that these TIMs were co-expressed with Langerin, a potential biomarker for detecting LCS. In addition, TIM-1 was also expressed on CD68+ macrophages and CK-18+ epithelial cells, while TIM-3 and TIM-4 were expressed on all cell types investigated, including CD3+T cells, CD68+ macrophages, CD11c+ dendritic cells, CD16+ NK Cells, CD31+ endothelial cells and CK-18+ epithelial cells. Interestingly, TIMs were also co-expressed with some members of the B7 superfamily, including B7-H1, B7-H3 and B7-H4 on sarcoma cells. Our results clearly showed the characteristic expression and anatomical distribution of TIMs in LCS, and a clear understanding of their functional roles may further elucidate the pathogenesis of this carcinoma and potentially contribute to the development of novel immunotherapeutic strategies.
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This work was supported by grants from the National Natural Science Foundation of China (NSFC No. 81171585, 30971099 and 61141012) and Natural Science Foundation of Chongqing (No CSTC2011BB5037).
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Jingwei Li and Dayan Cao equally contributed to this work.
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10735_2012_9475_MOESM1_ESM.tif
Supplmental Figure 1 The morphology of TIM-1 positive cells in LCS sample sections detected by immunofluorescence double-staining. Immunofluorescence double-staining showed that TIM-1 was expressed on CD68+ macrophages and CK-18+ epithelial cells, while it was absent on CD3+ T cells, CD11c+DCs, CD16+ monocytes, and CD31+ endothelial cells. Arrow indicates positive cells. Nuclei were stained with DAPI. Scale bar = 20 μm. (TIFF 6903 kb)
10735_2012_9475_MOESM2_ESM.tif
Supplmental Figure 2 The morphology of TIM-3positive cells in LCS sample sections detected by immunofluorescence double-staining. Immunofluorescence double-staining showed that TIM-3 was expressed on CD3+ T cells, CD68+ macrophages, CD11c+ dendritic cells, CD16+ monocytes, CD31+ endothelial cells and CK-18+ epithelial cells. Arrow indicates positive cells. Nuclei were stained with DAPI. Scale bar = 20 μm. (TIFF 7448 kb)
10735_2012_9475_MOESM3_ESM.tif
Supplmental Figure 3 The morphology of TIM-4positive cells in LCS sample sections was detected by immunofluorescence double-staining. Immunofluorescence double-staining showed that TIM-4 was expressed on CD3+ T cells, CD68+ macrophages, CD11c+ dendritic cells, CD16+ monocytes, CD31+ endothelial cells and CK-18+ epithelial cells. Arrow indicates positive cells. Nuclei were stained with DAPI. Scale bar = 20 μm. (TIFF 5579 kb)
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Li, J., Cao, D., Guo, G. et al. Expression and anatomical distribution of TIM-containing molecules in Langerhans cell sarcoma. J Mol Hist 44, 213–220 (2013). https://doi.org/10.1007/s10735-012-9475-2
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DOI: https://doi.org/10.1007/s10735-012-9475-2