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A Comparative Study of Interleukin-1β Production and P2x7 Expression After Atp Stimulation by Peripheral Blood Mononuclear Cells Isolated From Rheumatoid Arthritis Patients and Normal Healthy Controls

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Abstract

Interleukin 1 beta (IL-1β) is a proinflammatory cytokine that is considered to play an important role in the progression of rheumatoid arthritis (RA). A stimulus such as ATP is necessary to cause the release of mature IL-1β, via activation of the P2X7 receptor on monocytes. In this study, the production of IL-1β in whole blood after ATP stimulation and expression of P2X7 receptors in RA and healthy subjects were examined. Blood samples from RA patients or healthy controls were stimulated with ATP in the presence of lipopolysaccharide (LPS). Supernatants were harvested and IL-1β levels were measured by enzyme-linked immunosorbent assay (ELISA). Expression of P2X7 receptors was measured using flow cytometry. ATP induced significantly higher levels of IL-1β in LPS-activated RA blood samples compared to controls. A significant up-regulation of P2X7 receptor expression on mononuclear cells was observed after overnight incubation with ATP without any significant differences between RA patients and normals. These data suggest that RA patient mononuclear cells are more sensitive to ATP stimulation than healthy individuals perhaps due to genetic polymorphism in the P2X7 gene.

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Acknowledgment

We thank the Medical Research and Ethics Committee (MREC), College of Medicine and Health Sciences, Sultan Qaboos University, Muscat, Sultanate of Oman, for financially supporting this research. We also thank Professor Eugene H. Johnson and Professor Sandy Raeburn for their excellent editorial assistance and critical reading of the manuscript and Dr Abdullah. Al-Munari for his help in statistical analysis.

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Authors and Affiliations

  1. Department of Microbiology and Immunology, College of Medicine and Health Sciences, Sultan Qaboos University, P.O. Box 35, PC 123, Muscat, Sultanate of Oman

    Ahmed Al-Shukaili

  2. Department of Medicine, Rheumatology Unit, Sultan Qaboos University Hospital, P.O. Box 35, PC 123, Muscat, Sultanate of Oman

    Juma Al-Kaabi & Batool Hassan

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  1. Ahmed Al-Shukaili
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  2. Juma Al-Kaabi
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  3. Batool Hassan
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Correspondence to Ahmed Al-Shukaili.

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Al-Shukaili, A., Al-Kaabi, J. & Hassan, B. A Comparative Study of Interleukin-1β Production and P2x7 Expression After Atp Stimulation by Peripheral Blood Mononuclear Cells Isolated From Rheumatoid Arthritis Patients and Normal Healthy Controls. Inflammation 31, 84–90 (2008). https://doi.org/10.1007/s10753-007-9052-0

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  • DOI: https://doi.org/10.1007/s10753-007-9052-0

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