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Erschienen in: Inflammation 1/2014

01.02.2014

Thymol Inhibits LPS-Stimulated Inflammatory Response via Down-Regulation of NF-κB and MAPK Signaling Pathways in Mouse Mammary Epithelial Cells

verfasst von: Dejie Liang, Fengyang Li, Yunhe Fu, Yongguo Cao, Xiaojing Song, Tiancheng Wang, Wei Wang, Mengyao Guo, Ershun Zhou, Depeng Li, Zhengtao Yang, Naisheng Zhang

Erschienen in: Inflammation | Ausgabe 1/2014

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Abstract

Thymol is a natural monoterpene phenol primarily found in thyme, oregano, and tangerine peel. It has been shown to possess anti-inflammatory property both in vivo and in vitro. In the present paper, we studied the anti-inflammatory effect of thymol in lipopolysaccharide (LPS)-stimulated mouse mammary epithelial cells (mMECs). The mMECs were stimulated with LPS in the presence or absence of thymol (10, 20, 40 μg/mL). The concentrations of tumor necrosis factor α (TNF-α), interleukin (IL)-6, and IL-1β in the supernatants of culture were determined using enzyme-linked immunosorbent assay. Cyclooxygenase-2 (COX-2), inducible nitric oxide synthase (iNOS), extracellular signal-regulated protein kinase (ERK), c-Jun N-terminal kinase (JNK), nuclear factor-κB (NF-κB), and inhibitor protein of NF-κB (IκBα) were measured using western blot. The results showed that thymol markedly inhibited the production of TNF-α and IL-6 in LPS-stimulated mMECs. The expression of iNOS and COX-2 was also suppressed by thymol in a dose-dependent manner. Furthermore, thymol blocked the phosphorylation of IκBα, NF-κB p65, ERK, JNK, and p38 mitogen-activated protein kinases (MAPKs) in LPS-stimulated mMECs. These results indicate that thymol exerted anti-inflammatory property in LPS-stimulated mMECs by interfering the activation of NF-κB and MAPK signaling pathways. Thereby, thymol may be a potential therapeutic agent against mastitis.
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Metadaten
Titel
Thymol Inhibits LPS-Stimulated Inflammatory Response via Down-Regulation of NF-κB and MAPK Signaling Pathways in Mouse Mammary Epithelial Cells
verfasst von
Dejie Liang
Fengyang Li
Yunhe Fu
Yongguo Cao
Xiaojing Song
Tiancheng Wang
Wei Wang
Mengyao Guo
Ershun Zhou
Depeng Li
Zhengtao Yang
Naisheng Zhang
Publikationsdatum
01.02.2014
Verlag
Springer US
Erschienen in
Inflammation / Ausgabe 1/2014
Print ISSN: 0360-3997
Elektronische ISSN: 1573-2576
DOI
https://doi.org/10.1007/s10753-013-9732-x

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