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Erythropoietin Reduces Acute Lung Injury and Multiple Organ Failure/Dysfunction Associated to a Scald-Burn Inflammatory Injury in the Rat

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Abstract

Erythropoietin (EPO) is an endogenous regulator of erythropoiesis and is given exogenously as a replacement therapy for selected red blood cell disorders. In the past years, EPO has been emerging as a multifunctional, cytoprotective cytokine with anti-apoptotic, anti-inflammatory, and immunomodulatory properties. We aimed to evaluate the cytoprotective effect of rhEPO (recombinant human EPO) treatment on a rat model of multiorgan dysfunction induced by thermal injury. rhEPO was administered at 1000 U/kg (i.v.) 5 min prior to induction of injury and significantly reduced multiorgan dysfunction markers (liver, kidney, lung, serum cytokine levels). In the lung, rhEPO reduced: histological signs of tissue injury, inflammatory/injury markers on the bronchoalveolar fluid, neutrophil chemotaxis/infiltration, GSK-3β activation, and apoptosis. Our study showed that erythropoietin has the potential to exhibit pleiotropic cytoprotective effects and that it might be an interesting pharmacological strategy in the modulation of acute lung injury, such as the one associated to severe burn.

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ACKNOWLEDGMENTS

João Rocha was supported by FCT under a Doctoral Grant (SFRH /BD/64180/2009). Marisa Freitas acknowledges FCT the financial support for the Pos-doc grant (SFRH/BPD/76909/2011), in the scope of “QREN e POPH e Tipologia 4.1 e Formação Avançada”, co-sponsored by FSE and by National Funds of MCTES.

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  1. Research Institute for Medicines (iMed.ULisboa), Avenida Prof. Gama Pinto, 1649-003, Lisbon, Portugal

    Joao Rocha, Maria Eduardo-Figueira, Andreia Barateiro, Adelaide Fernandes, Dora Brites, Rui Pinto, Helder Mota-Filipe & Bruno Sepodes

  2. REQUIMTE, Laboratório de Química Aplicada, Departamento de Ciências Químicas, Faculdade de Farmácia, Universidade do Porto, 4050-313, Porto, Portugal

    Marisa Freitas & Eduarda Fernandes

  3. Faculdade de Farmácia, Universidade de Lisboa, Avenida Prof. Gama Pinto, 1649-003, Lisbon, Portugal

    Bruno Sepodes

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  1. Joao Rocha
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Rocha, J., Eduardo-Figueira, M., Barateiro, A. et al. Erythropoietin Reduces Acute Lung Injury and Multiple Organ Failure/Dysfunction Associated to a Scald-Burn Inflammatory Injury in the Rat. Inflammation 38, 312–326 (2015). https://doi.org/10.1007/s10753-014-0035-7

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