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Inflammopharmacology

Erschienen in:

05.07.2018 | Original Article

Triptolide prevents osteoarthritis via inhibiting hsa-miR-20b

verfasst von: Kejun Qian, Li Zhang, Keqin Shi

Erschienen in: Inflammopharmacology | Ausgabe 1/2019

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Abstract

Triptolide has been widely reported to exhibit potential therapeutic value in multiple inflammatory diseases, such as rheumatoid arthritis, systemic lupus erythematosus, and psoriasis. Although its safety and efficacy as an anti-inflammatory agent have been verified by many studies, the effect of triptolide on osteoarthritis (OA) was not clearly understood. In this study, we found that triptolide prevented OA development in a surgical destabilization of the medial meniscus (DMM) mouse model. In addition, triptolide inhibited both DMM-induced and LPS-induced expression of pro-inflammatory cytokines in the human monocytic cell line THP-1. Further mechanistic studies showed that the reduction of pro-inflammatory cytokines by triptolide was mediated by the upregulation of nucleotide-binding oligomerization domain-like receptor family pyrin domain-containing protein 3 (NLRP3) and downregulation of caspase-1. Finally, we identified that hsa-miR-20b, a microRNA targeting the NLRP3 gene, was downregulated by triptolide. This study provides a novel insight into the effect on triptolide in preventing OA pathogenesis.

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Titel: Triptolide prevents osteoarthritis via inhibiting hsa-miR-20b
verfasst von: Kejun Qian
Li Zhang
Keqin Shi
Publikationsdatum: 05.07.2018
Verlag: Springer International Publishing
Erschienen in: Inflammopharmacology / Ausgabe 1/2019
Print ISSN: 0925-4692
Elektronische ISSN: 1568-5608
DOI: https://doi.org/10.1007/s10787-018-0509-6

Springer Medizin

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