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Journal of Assisted Reproduction and Genetics

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01.10.2009 | Andrology

Oxidative DNA damage impairs global sperm DNA methylation in infertile men

verfasst von: Ozlem Tunc, Kelton Tremellen

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 9-10/2009

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Abstract

Purpose

Methylation of sperm DNA is impaired in many infertile men potentially adversely effecting reproductive outcomes. In somatic cells oxidative damage to DNA and hyperhomocysteinaemia are linked with DNA hypomethylation. The objective of this study was to investigate if these pathologies also impair sperm DNA methylation.

Methods

The relationship between sperm DNA quality, oxidative stress and serum homocysteine was analysed at study entry and after 3 months of antioxidant treatment.

Results

Overall a significant negative correlation was observed between sperm DNA methylation and sperm DNA fragmentation, as well as seminal reactive oxygen species (ROS) production. Sperm DNA methylation was not significantly related to serum homocysteine concentrations. Administration of an antioxidant supplement produced a significant fall in seminal ROS levels and sperm DNA fragmentation, while increasing sperm DNA methylation.

Conclusions

These results suggest that oxidative stress related damage to sperm DNA impedes the process of methylation, while antioxidant supplementation appears to have the potential to reduce DNA damage and normalize sperm DNA methylation.

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Titel: Oxidative DNA damage impairs global sperm DNA methylation in infertile men
verfasst von: Ozlem Tunc
Kelton Tremellen
Publikationsdatum: 01.10.2009
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 9-10/2009
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-009-9346-2

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Oxidative DNA damage impairs global sperm DNA methylation in infertile men

Abstract

Purpose

Methods

Results

Conclusions

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