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Journal of Assisted Reproduction and Genetics

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01.02.2010 | GAMETE BIOLOGY

Expression of the Fas-ligand gene in ejaculated sperm from adolescents with and without varicocele

verfasst von: Paula Toni Del Giudice, Samira Barbosa Lima, Marcos Antonio Cenedeze, Álvaro Pacheco-Silva, Ricardo Pimenta Bertolla, Agnaldo Pereira Cedenho

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 2-3/2010

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Abstract

Purpose

To assess FasL mRNA levels in ejaculated sperm from adolescent patients with and without varicocele.

Methods

Semen was obtained by masturbation following 2–4 days of ejaculatory abstinence, from 14 adolescents with varicocele grades II and III (study group), and 20 adolescents without varicocele (control group). Seminal analysis was done according to World Health Organization guidelines and morphology using Kruger’s strict criteria. The Fas-ligand (FasL) gene expression was performed using reverse transcription and real-time quantitative polymerase chain reaction (RQ-PCR) analysis, according to the expression level of the housekeeping cyclophilin A gene. A Student’s t-test was applied to compare the groups, and Spearman’s rank test in order to verify possible correlations (p < 0.05).

Results

Quantitative RQ-PCR demonstrated that the expression of FasL mRNA in sperm from the varicocele group was higher than in the control group. Also, sperm concentration was higher in the controls, when compared to the varicocele group. When submitted to correlation analysis, adolescents with varicocele presented a correlation between sperm concentration and FasL gene expression levels (r = −0.470), not observed in controls.

Conclusion

Our results allow us to conclude that, in adolescents with varicocele presenting lower sperm concentration, FasL mRNA levels are higher than in adolescents without varicocele.

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Titel: Expression of the Fas-ligand gene in ejaculated sperm from adolescents with and without varicocele
verfasst von: Paula Toni Del Giudice
Samira Barbosa Lima
Marcos Antonio Cenedeze
Álvaro Pacheco-Silva
Ricardo Pimenta Bertolla
Agnaldo Pereira Cedenho
Publikationsdatum: 01.02.2010
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 2-3/2010
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-010-9384-9

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Expression of the Fas-ligand gene in ejaculated sperm from adolescents with and without varicocele

Abstract

Purpose

Methods

Results

Conclusion

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Abstract

Purpose

Methods

Results

Conclusion

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