nach oben

Journal of Assisted Reproduction and Genetics

Erschienen in:

01.06.2010 | Gamete Biology

The expression pattern of SEPT7 correlates with sperm morphology

verfasst von: Hsin-Chih Albert Chao, Ying-Hung Lin, Yung-Che Kuo, Chiung-Jiung Shen, Hsian-Ann Pan, Pao-Lin Kuo

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 6/2010

Einloggen, um Zugang zu erhalten

Abstract

Purpose

To investigate the expression pattern of the SEPT7 protein during spermatogenesis and its potential role in sperm function.

Methods

We first investigated the expression pattern of SEPT7 during different steps of mouse spermiogenesis using an immunofluorescence assay (IFA). IFA was also applied to study the expression pattern of SEPT7 in human ejaculated spermatozoa. Nine fertile men with normal semen parameters were used as the control group, and 21 infertile men with asthenozoospermia were recruited as the patient group. We assessed the frequency of the SEPT7 signal in the various morphological subgroups.

Results

In humans, the frequency of a defective SEPT7 signal was significantly increased in men with asthenozoospermia. The absence of a SEPT7 signal was more prevalent in sperm containing morphological defects of various types.

Conclusions

The expression pattern of SEPT7 suggested that this protein may be involved in the regulation of subcellular-compartment formation during spermiogenesis in the mouse. The absence of a SEPT7 signal correlated with multiple sperm defects.

Hall PA, Russell SE. The pathobiology of the septin gene family. J Pathol. 2004;204:489–505.CrossRefPubMed

Elhasid R, Sahar D, Merling A, Zivony Y, Rotem A, Ben-Arush M, et al. Mitochondrial pro-apoptotic ARTS protein is lost in the majority of acute lymphoblastic leukemia patients. Oncogene. 2004;23:5468–75.CrossRefPubMed

Takahashi S, Inatome R, Yamamura H, Yanagi S. Isolation and expression of a novel mitochondrial septin that interacts with CRMP/CRAM in the developing neurones. Genes Cells. 2003;8:81–93.CrossRefPubMed

Larisch S, Yi Y, Lotan R, Kerner H, Eimerl S, Parks WT, et al. A novel mitochondrial septin-like protein, ARTS, mediates apoptosis dependent on its P-loop motif. Nat Cell Biol. 2000;2:915–21.CrossRefPubMed

Hall PA, Jung K, Hillan KJ, Russell SE. Expression profiling the human septin gene family. J Pathol. 2005;206:269–78.CrossRefPubMed

Steels JD, Estey MP, Froese CD, Reynaud D, Pace-Asciak C, Trimble WS. Sept12 is a component of the mammalian sperm tail annulus. Cell Motil Cytoskeleton. 2007;64:794–807.CrossRefPubMed

Kissel H, Georgescu MM, Larisch S, Manova K, Hunnicutt GR, Steller H. The Sept4 septin locus is required for sperm terminal differentiation in mice. Dev Cell. 2005;8:353–64.CrossRefPubMed

Ihara M, Kinoshita A, Yamada S, Tanaka H, Tanigaki A, Kitano A, et al. Cortical organization by the septin cytoskeleton is essential for structural and mechanical integrity of mammalian spermatozoa. Dev Cell. 2005;8:343–52.CrossRefPubMed

Lhuillier P, Rode B, Escalier D, Lores P, Dirami T, Bienvenu T, et al. Absence of annulus in human asthenozoospermia: case report. Hum Reprod. 2009;24:1296–303.CrossRefPubMed

10.

Sugino Y, Ichioka K, Soda T, Ihara M, Kinoshita M, Ogawa O, et al. Septins as diagnostic markers for a subset of human asthenozoospermia. J Urol. 2008;180:2706–9.CrossRefPubMed

11.

Lin YH, Lin YM, Wang YY, Yu IS, Lin YW, Wang YH, et al. The expression level of septin12 is critical for spermiogenesis. Am J Pathol. 2009;174:1857–68.CrossRefPubMed

12.

Tada T, Simonetta A, Batterton M, Kinoshita M, Edbauer D, Sheng M. Role of septin cytoskeleton in spine morphogenesis and dendrite development in neurons. Curr Biol. 2007;17:1752–8.CrossRefPubMed

13.

Rawe VY, Ramalho-Santos J, Payne C, Chemes HE, Schatten G. WAVE1, an A-kinase anchoring protein, during mammalian spermatogenesis. Hum Reprod. 2004;19:2594–604.CrossRefPubMed

14.

Lin YH, Lin YM, Teng YN, Hsieh TY, Lin YS, Kuo PL. Identification of ten novel genes involved in human spermatogenesis by microarray analysis of testicular tissue. Fertil Steril. 2006;86:1650–8.CrossRefPubMed

15.

Yeh YC, Yang VC, Huang SC, Lo NW. Stage-dependent expression of extra-embryonic tissue-spermatogenesis-homeobox gene 1 (ESX1) protein, a candidate marker for X chromosome-bearing sperm. Reprod Fertil Dev. 2005;17:447–55.CrossRefPubMed

16.

Greenbaum MP, Ma L, Matzuk MM. Conversion of midbodies into germ cell intercellular bridges. Dev Biol. 2007;305:389–96.CrossRefPubMed

17.

Fawcett DW, Anderson WA, Phillips DM. Morphogenetic factors influencing the shape of the sperm head. Dev Biol. 1971;26:220–51.CrossRefPubMed

18.

Russell LD, Russell JA, MacGregor GR, Meistrich ML. Linkage of manchette microtubules to the nuclear envelope and observations of the role of the manchette in nuclear shaping during spermiogenesis in rodents. Am J Anat. 1991;192:97–120.CrossRefPubMed

19.

Akhmanova A, Mausset-Bonnefont AL, van Cappellen W, Keijzer N, Hoogenraad CC, Stepanova T, et al. The microtubule plus-end-tracking protein CLIP-170 associates with the spermatid manchette and is essential for spermatogenesis. Genes Dev. 2005;19:2501–15.CrossRefPubMed

20.

Kato A, Nagata Y, Todokoro K. Delta-tubulin is a component of intercellular bridges and both the early and mature perinuclear rings during spermatogenesis. Dev Biol. 2004;269:196–205.CrossRefPubMed

21.

Nagata K, Kawajiri A, Matsui S, Takagishi M, Shiromizu T, Saitoh N, et al. Filament formation of MSF-A, a mammalian septin, in human mammary epithelial cells depends on interactions with microtubules. J Biol Chem. 2003;278:18538–43.CrossRefPubMed

22.

Surka MC, Tsang CW, Trimble WS. The mammalian septin MSF localizes with microtubules and is required for completion of cytokinesis. Mol Biol Cell. 2002;13:3532–45.CrossRefPubMed

23.

Caudron F, Barral Y. Septins and the lateral compartmentalization of eukaryotic membranes. Dev Cell. 2009;16:493–506.CrossRefPubMed

24.

Guan J, Kinoshita M, Yuan L. Spatiotemporal association of DNAJB13 with the annulus during mouse sperm flagellum development. BMC Dev Biol. 2009;9:23.CrossRefPubMed

25.

Toure A, Lhuillier P, Gossen JA, Kuil CW, Lhote D, Jegou B, et al. The testis anion transporter 1 (Slc26a8) is required for sperm terminal differentiation and male fertility in the mouse. Hum Mol Genet. 2007;16:1783–93.CrossRefPubMed

26.

Escalier D. Arrest of flagellum morphogenesis with fibrous sheath immaturity of human spermatozoa. Andrologia. 2006;38:54–60.CrossRefPubMed

27.

Turner TT. On the epididymis and its role in the development of the fertile ejaculate. J Androl. 1995;16:292–8.PubMed

28.

Cooper TG. Cytoplasmic droplets: the good, the bad or just confusing? Hum Reprod (Oxford, England). 2005;20:9–11.CrossRef

29.

Gergely A, Kovanci E, Senturk L, Cosmi E, Vigue L, Huszar G. Morphometric assessment of mature and diminished-maturity human spermatozoa: sperm regions that reflect differences in maturity. Hum Reprod. 1999;14:2007–14.CrossRefPubMed

30.

Huszar G, Vigue L. Incomplete development of human spermatozoa is associated with increased creatine phosphokinase concentration and abnormal head morphology. Mol Reprod Dev. 1993;34:292–8.CrossRefPubMed

31.

Huszar G, Vigue L. Spermatogenesis-related change in the synthesis of the creatine kinase B-type and M-type isoforms in human spermatozoa. Mol Reprod Dev. 1990;25:258–62.CrossRefPubMed

32.

Huszar G, Vigue L, Corrales M. Sperm creatine kinase activity in fertile and infertile oligospermic men. J Androl. 1990;11:40–6.PubMed

33.

Cooper TG, Yeung CH. Acquisition of volume regulatory response of sperm upon maturation in the epididymis and the role of the cytoplasmic droplet. Microsc Res Tech. 2003;61:28–38.CrossRefPubMed

34.

Nagata K, Asano T, Nozawa Y, Inagaki M. Biochemical and cell biological analyses of a mammalian septin complex, Sept7/9b/11. J Biol Chem. 2004;279:55895–904.CrossRefPubMed

35.

Cesario MM, Bartles JR. Compartmentalization, processing and redistribution of the plasma membrane protein CE9 on rodent spermatozoa. Relationship of the annulus to domain boundaries in the plasma membrane of the tail. J Cell Sci. 1994;107(Pt 2):561–70.PubMed

36.

Takizawa PA, DeRisi JL, Wilhelm JE, Vale RD. Plasma membrane compartmentalization in yeast by messenger RNA transport and a septin diffusion barrier. Science. 2000;290:341–4.CrossRefPubMed

Titel: The expression pattern of SEPT7 correlates with sperm morphology
verfasst von: Hsin-Chih Albert Chao
Ying-Hung Lin
Yung-Che Kuo
Chiung-Jiung Shen
Hsian-Ann Pan
Pao-Lin Kuo
Publikationsdatum: 01.06.2010
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 6/2010
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-010-9409-4

Neu im Fachgebiet Gynäkologie und Geburtshilfe

17.04.2024 | Postoperative nausea and vomiting | Nachrichten

Update Gynäkologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert – ganz bequem per eMail.

Newsletter bestellen

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

The expression pattern of SEPT7 correlates with sperm morphology

Abstract

Purpose

Methods

Results

Conclusions

Neu im Fachgebiet Gynäkologie und Geburtshilfe

Hochrisiko-Eingriffe für postoperative Übelkeit

Adjuvante Brustkrebstherapie: Doppelt hält besser

Manche Gestagene können das Risiko für Meningeome erhöhen

Stillende Frauen haben geringeres kardiovaskuläres Risiko

Update Gynäkologie

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

Purpose

Methods

Results

Conclusions

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 6/2010

Increased apoptosis of germ cells in patients with AZFc deletions

Erratum to: Kinetics of human male pronuclear development in a heterologous ICSI model

Kinetics of human male pronuclear development in a heterologous ICSI model

Revisiting “on meetings that matter”

The single cell as a tool for genetic testing: credibility, precision, implication

Derivation and long-term culture of human parthenogenetic embryonic stem cells using human foreskin feeders

Neu im Fachgebiet Gynäkologie und Geburtshilfe

Hochrisiko-Eingriffe für postoperative Übelkeit

Adjuvante Brustkrebstherapie: Doppelt hält besser

Manche Gestagene können das Risiko für Meningeome erhöhen

Stillende Frauen haben geringeres kardiovaskuläres Risiko

Update Gynäkologie