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Journal of Assisted Reproduction and Genetics

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01.09.2011 | Fertility Preservation

Alginate scaffold for organ culture of cryopreserved-thawed human ovarian cortical follicles

verfasst von: Alon Kedem, Ariel Hourvitz, Benjamin Fisch, Michal Shachar, Smadar Cohen, Avi Ben-Haroush, Joshua Dor, Enrique Freud, Carmela Felz, Ronit Abir

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 9/2011

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Abstract

Purpose

To compare macroporous alginate scaffolds with Matrigel for culturing frozen-thawed human primordial follicles in organ culture.

Methods

Twelve girls/women donated ovarian tissue. One tissue sample was fixed immediately after thawing (uncultured samples). Slices were cultured for 2 weeks on either Matrigel or on alginate scaffolds with a serum-free culture medium. Growth evaluation consisted of follicular counts and classification, immunohistochemistry and measurement of 17β-Estradiol (E₂) production.

Results

The number of developing follicles was significantly higher in alginate scaffold-cultured samples than on Matrigel with a concomitant decrease in the number of primordial follicles in alginate scaffold-cultured samples than uncultured samples. The number of atretic follicles after 1 week was significantly higher in the Matrigel-cultured samples than in the alginate scaffold cultured samples. E₂ production was similar in both groups.

Conclusions

Three dimensional alginate scaffolds are a promising putative in vitro technology for developing human primordial follicles.

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Titel: Alginate scaffold for organ culture of cryopreserved-thawed human ovarian cortical follicles
verfasst von: Alon Kedem
Ariel Hourvitz
Benjamin Fisch
Michal Shachar
Smadar Cohen
Avi Ben-Haroush
Joshua Dor
Enrique Freud
Carmela Felz
Ronit Abir
Publikationsdatum: 01.09.2011
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 9/2011
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-011-9605-x

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