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Journal of Assisted Reproduction and Genetics

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01.12.2011 | Gamete Biology

Maturation outcomes are improved following Cryoleaf vitrification of immature human oocytes when compared to choline-based slow-freezing

verfasst von: Catherine M. H. Combelles, S. Temel Ceyhan, Haiyan Wang, Catherine Racowsky

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 12/2011

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Abstract

Purpose

The cryopreservation of immature oocytes permits oocyte banking for patients at risk of losing their fertility. However, the optimum protocol for such fertility preservation remains uncertain.

Methods

The present study investigated the survival, maturation, cytoskeletal and chromosome organization of sibling immature oocytes leftover from controlled ovarian stimulation cycles, that were either slow-frozen (with choline-substitution) or vitrified. A comparison group included oocytes that were never cryopreserved.

Results

Among the three groups, comparable rates were observed for both survival (67-70%) and polar body extrusion (59-79%). Significantly more oocytes underwent spontaneous activation after IVM following slow-freezing compared with either vitrification or no cryopreservation. Likewise, the incidence of spindle abnormalities was greatest in the slow-frozen group, with no differences in spindle morphometrics or chromosome organization.

Conclusions

While the overall incidence of mature oocytes with normal bipolar spindles from warmed immature oocytes was low, the yield using Cryoleaf vitrification was slightly superior to choline-based slow-freezing.

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Titel: Maturation outcomes are improved following Cryoleaf vitrification of immature human oocytes when compared to choline-based slow-freezing
verfasst von: Catherine M. H. Combelles
S. Temel Ceyhan
Haiyan Wang
Catherine Racowsky
Publikationsdatum: 01.12.2011
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 12/2011
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-011-9674-x

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Maturation outcomes are improved following Cryoleaf vitrification of immature human oocytes when compared to choline-based slow-freezing

Abstract

Purpose

Methods

Results

Conclusions

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Methods

Results

Conclusions

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