Anzeige
Erschienen in:
01.09.2012 | ASSISTED REPRODUCTION TECHNOLOGIES
Time-lapse evaluation of human embryo development in single versus sequential culture media—a sibling oocyte study
Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 9/2012
Einloggen, um Zugang zu erhaltenAbstract
Objective
To compare the dynamics of early development between embryos cultured in single and sequential media.
Design
Randomized, comparative study.
Setting
Private IVF centre.
Patients
A total of 446 metaphase II oocytes from 51 couples who underwent oocyte retrieval procedure for intracytoplasmic sperm injection. Forty-nine resulted in embryo transfer.
Intervention
Oocytes were split between single and sequential media produced by the same manufacturer and cultured in a time-lapse incubator.
Main outcome measures
Morphokinetic parameters until the embryos reached the 5-cell stage (t5), utilization, clinical pregnancy and implantation rates.
Results
Embryos cultured in single media were advanced from the first mitosis cycle and reached 2- to 5-cell stages earlier. There was not any difference between the durations for cell cycle two (cc2 = t3-t2) and s2 (t4-t3). The utilization, clinical pregnancy and implantation rates did not differ between groups. The proportion of cryopreserved day6 embryos to two pronuclei oocytes was significantly higher in sequential than in single media.
Conclusions
Morphokinetics of embryo development vary between single and sequential culture media at least until the 5-cell stage. The overall clinical and embryological parameters remain similar regardless of the culture system.