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Journal of Assisted Reproduction and Genetics

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01.10.2012 | Gamete Biology

Effect of vitrification on mitochondrial membrane potential in human metaphase II oocytes

verfasst von: Cui Chen, Shubiao Han, Weiwei Liu, Yaping Wang, Guoning Huang

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 10/2012

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Abstract

Objective

The aim of this study was to evaluate the impact of vitrification on mitochondrial membrane potential (ΔΨm) in human metaphase II (MII) oocytes, and the changes of ΔΨm on thawed MII oocytes.

Methods

MII oocytes were obtained from clinical IVF cycles when the oocytes were failed to fertilization within 24 h after insemination. All oocytes were randomly divided into 4 groups: non-frozen (fresh group), cultured for 0 h (0 h group), 2 h (2 h group) and 4 h (4 h group) after vitrification/thawing. All oocytes were stained with the ΔΨm-specific probe JC-1 and detected by laser scanning confocal microscope (LSCM) for mitochondrial analysis.

Results

The ΔΨm of oocytes was significantly decreased in 0 h and 2 h groups when compared with fresh group (0.93, 1.09 vs 1.34, P < 0.05), but similar between 4 h group and fresh group (1.30 vs 1.34, P > 0.05).

Conclusion

In the vitrification/thawing process, the ΔΨm of MII oocytes could have temporally dynamic changes within 2 h after thawing but would be fully recovered after 4 h culture.

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Titel: Effect of vitrification on mitochondrial membrane potential in human metaphase II oocytes
verfasst von: Cui Chen
Shubiao Han
Weiwei Liu
Yaping Wang
Guoning Huang
Publikationsdatum: 01.10.2012
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 10/2012
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-012-9848-1

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Effect of vitrification on mitochondrial membrane potential in human metaphase II oocytes

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Objective

Methods

Results

Conclusion

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Springer Medizin

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