Skip to main content
Erschienen in: Journal of Assisted Reproduction and Genetics 1/2013

01.01.2013 | ASSISTED REPRODUCTION TECHNOLOGIES

Fertilization, embryo development, and clinical outcome of immature oocytes obtained from natural cycle in vitro fertilization

verfasst von: Weon-Young Son, Jin-Tae Chung, Mausumi Das, William Buckett, Ezgi Demirtas, Hananel Holzer

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 1/2013

Einloggen, um Zugang zu erhalten

Abstract

Purpose

To analyze the fertilization, embryo development, and clinical outcome of immature oocytes obtained from natural cycle IVF in women with regular cycles.

Methods

Natural cycle IVF was performed in 28 patients who had normal ovaries, > 6 antral follicle counts and were less than 40 years old (n = 28 cycles). An hCG trigger of 10,000 IU was administered 36 h before oocyte collection when the diameter of the dominant follicle (DF) was over 12 mm. Oocytes were retrieved from DF as well as from the cohort of smaller follicles. Embryological aspects of the mature and immature oocytes retrieved from these cycles as well as the implantation and clinical pregnancy rates depending on the origin of the embryos transferred were evaluated.

Result(s)

Overall clinical pregnancy and implantation rates were 20.8 % and 6.7 %, respectively. There were no differences in in vitro maturation (IVM), fertilization and embryo development between immature oocytes retrieved with and without in vivo matured oocytes. However, the clinical and implantation rates in cycles with embryos produced from in vivo matured oocytes transferred were better than the cycles where only IVM embryos were transferred (30.8 %, 9.1 % vs. 9.1 %, 3.2 %).

Conclusion(s)

Although our results show that immature oocytes from natural cycle IVF can fertilize normally and can be used to increase the number of embryos available for transfer, the embryos derived from the immature oocytes in natural cycles IVF have a poorer reproductive potential.
Literatur
1.
Zurück zum Zitat Steptoe PC, Edwards RG. Birth after the reimplantation of a human embryo. Lancet. 1978;2(8085):366.PubMedCrossRef Steptoe PC, Edwards RG. Birth after the reimplantation of a human embryo. Lancet. 1978;2(8085):366.PubMedCrossRef
2.
Zurück zum Zitat MacDougall MJ, Tan SL, Hall V, Balen A, Mason BA, Jacobs HS. Comparison of natural with clomiphene citrate-stimulated cycles in in-vitro fertilization: a prospective, randomized trial. Fertil Steril. 1994;61:1052–7.PubMed MacDougall MJ, Tan SL, Hall V, Balen A, Mason BA, Jacobs HS. Comparison of natural with clomiphene citrate-stimulated cycles in in-vitro fertilization: a prospective, randomized trial. Fertil Steril. 1994;61:1052–7.PubMed
3.
Zurück zum Zitat Janssens RM, Lambalk CB, Vermeiden JP, Schats R, Bernards JM, et al. In-vitro fertilization in a spontaneous cycle:easy, cheap and realistic. Hum Reprod. 2000;15:314–8.PubMedCrossRef Janssens RM, Lambalk CB, Vermeiden JP, Schats R, Bernards JM, et al. In-vitro fertilization in a spontaneous cycle:easy, cheap and realistic. Hum Reprod. 2000;15:314–8.PubMedCrossRef
4.
Zurück zum Zitat Johnston I, Lopata A, Speirs A, Hoult I, Kellow G, du Plessis Y. In vitro fertilization: the challenge of the eighties. Fertil Steril. 1981;36:699–706.PubMed Johnston I, Lopata A, Speirs A, Hoult I, Kellow G, du Plessis Y. In vitro fertilization: the challenge of the eighties. Fertil Steril. 1981;36:699–706.PubMed
5.
Zurück zum Zitat Lopata A, Brown JB, Leeton JF, Talbot JM, Wood C. In vitro fertilization of preovulatory oocytes and embryo transfer in infertile patients treatedwith clomiphene and human chorionic gonadotropin. Fertil Steril. 1978;30:27–35.PubMed Lopata A, Brown JB, Leeton JF, Talbot JM, Wood C. In vitro fertilization of preovulatory oocytes and embryo transfer in infertile patients treatedwith clomiphene and human chorionic gonadotropin. Fertil Steril. 1978;30:27–35.PubMed
6.
Zurück zum Zitat Edwards RG. IVF, IVM, natural cycle IVF, minimal stimulation IVF – time for a rethink. Reprod Biomed Online. 2007;15:106–19.PubMedCrossRef Edwards RG. IVF, IVM, natural cycle IVF, minimal stimulation IVF – time for a rethink. Reprod Biomed Online. 2007;15:106–19.PubMedCrossRef
7.
Zurück zum Zitat Chian RC, Buckett WM, Abdul Jalil AK, Son WY, Sylvestre C, Rao D, et al. Natural-cycle in vitro fertilization combined with in vitro maturation of immature oocytes is a potential approach in infertility treatment. Fertil Steril. 2004;82:1675–8.PubMedCrossRef Chian RC, Buckett WM, Abdul Jalil AK, Son WY, Sylvestre C, Rao D, et al. Natural-cycle in vitro fertilization combined with in vitro maturation of immature oocytes is a potential approach in infertility treatment. Fertil Steril. 2004;82:1675–8.PubMedCrossRef
8.
Zurück zum Zitat Chian RC, Lim JH, Tan SL. State of the art in in-vitro oocyte maturation. Curr Opin Obstet Gynecol. 2004;16:211–9.PubMedCrossRef Chian RC, Lim JH, Tan SL. State of the art in in-vitro oocyte maturation. Curr Opin Obstet Gynecol. 2004;16:211–9.PubMedCrossRef
9.
Zurück zum Zitat Lim JH, Park SY, Yoon SH, Yang SH, Chian RC. Combination of natural cycle IVF with IVM as infertility treatment. In: Tan SL, Chian RC, Buckett WM, editors. In-vitro maturation of human oocytes: basic science to clinical application. London: Informa Healthcare Press; 2007. p. 353–60. Lim JH, Park SY, Yoon SH, Yang SH, Chian RC. Combination of natural cycle IVF with IVM as infertility treatment. In: Tan SL, Chian RC, Buckett WM, editors. In-vitro maturation of human oocytes: basic science to clinical application. London: Informa Healthcare Press; 2007. p. 353–60.
10.
Zurück zum Zitat Lim JH, Yang SH, Xu Y, Yoon SH, Chian RC. Selection of patients for natural cycle in vitro fertilization combined with in vitro maturation of immature oocytes. Fertil Steril. 2009;91:1050–5.PubMedCrossRef Lim JH, Yang SH, Xu Y, Yoon SH, Chian RC. Selection of patients for natural cycle in vitro fertilization combined with in vitro maturation of immature oocytes. Fertil Steril. 2009;91:1050–5.PubMedCrossRef
11.
Zurück zum Zitat Yang SH, Patrizio P, Yoon SH, Lim JH, Chian RC. Comparison of pregnancy outcomes in natural cycle IVF/M treatment with or without mature oocytes retrieved at time of egg collection. Syst Biol Reprod Med. 2012;58:154–9.PubMedCrossRef Yang SH, Patrizio P, Yoon SH, Lim JH, Chian RC. Comparison of pregnancy outcomes in natural cycle IVF/M treatment with or without mature oocytes retrieved at time of egg collection. Syst Biol Reprod Med. 2012;58:154–9.PubMedCrossRef
12.
Zurück zum Zitat Son WY, Tan SL. Laboratory and embryological aspects of hCG-primed in vitro maturation cycles for patients with polycystic ovaries. Hum Reprod Update. 2010;16:675–89.PubMedCrossRef Son WY, Tan SL. Laboratory and embryological aspects of hCG-primed in vitro maturation cycles for patients with polycystic ovaries. Hum Reprod Update. 2010;16:675–89.PubMedCrossRef
13.
Zurück zum Zitat Hyun CS, Cha JH, Son WY, Yoon SH, Kim KA, Lim JH. Optimal ICSI timing after the first polar body extrusion in in vitro matured human oocytes. Hum Reprod. 2007;22:1991–5.PubMedCrossRef Hyun CS, Cha JH, Son WY, Yoon SH, Kim KA, Lim JH. Optimal ICSI timing after the first polar body extrusion in in vitro matured human oocytes. Hum Reprod. 2007;22:1991–5.PubMedCrossRef
14.
Zurück zum Zitat Ginther OJ, Beg MA, Bergfelt DR, Donadeu FX, Kot K. Follicle selection in monovular species. Biol Reprod. 2001;65:638–47.PubMedCrossRef Ginther OJ, Beg MA, Bergfelt DR, Donadeu FX, Kot K. Follicle selection in monovular species. Biol Reprod. 2001;65:638–47.PubMedCrossRef
16.
Zurück zum Zitat Chian RC, Chung JT, Downey BR, Tan SL. Maturational and developmental competence of immature oocytes retrieved from bovine ovaries at different phases of folliculogenesis. Reprod Biomed Online. 2002;4:127–32.PubMedCrossRef Chian RC, Chung JT, Downey BR, Tan SL. Maturational and developmental competence of immature oocytes retrieved from bovine ovaries at different phases of folliculogenesis. Reprod Biomed Online. 2002;4:127–32.PubMedCrossRef
17.
Zurück zum Zitat Thornton MH, Francis MM, Paulson RJ. Immature oocyte retrieval: lessons from unstimulated IVF cycles. Fertil Steril. 1998;70:647–50.PubMedCrossRef Thornton MH, Francis MM, Paulson RJ. Immature oocyte retrieval: lessons from unstimulated IVF cycles. Fertil Steril. 1998;70:647–50.PubMedCrossRef
18.
Zurück zum Zitat Son WY, Chung JT, Herrero B, Dean N, Demirtas E, Holzer H, et al. Selection of the optimal day for oocyte retrieval based on the diameter of the dominant follicle in hCG-primed in vitro maturation cycles. Hum Reprod. 2008;23:2680–5.PubMedCrossRef Son WY, Chung JT, Herrero B, Dean N, Demirtas E, Holzer H, et al. Selection of the optimal day for oocyte retrieval based on the diameter of the dominant follicle in hCG-primed in vitro maturation cycles. Hum Reprod. 2008;23:2680–5.PubMedCrossRef
19.
Zurück zum Zitat Paulson RJ, Sauer MV, Francis MM, Macaso T, Lobo RA. Factors affecting pregnancy success of human in-vitro fertilization in unstimulated cycles. Hum Reprod. 1994;9:1571–5.PubMed Paulson RJ, Sauer MV, Francis MM, Macaso T, Lobo RA. Factors affecting pregnancy success of human in-vitro fertilization in unstimulated cycles. Hum Reprod. 1994;9:1571–5.PubMed
Metadaten
Titel
Fertilization, embryo development, and clinical outcome of immature oocytes obtained from natural cycle in vitro fertilization
verfasst von
Weon-Young Son
Jin-Tae Chung
Mausumi Das
William Buckett
Ezgi Demirtas
Hananel Holzer
Publikationsdatum
01.01.2013
Verlag
Springer US
Erschienen in
Journal of Assisted Reproduction and Genetics / Ausgabe 1/2013
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-012-9889-5

Weitere Artikel der Ausgabe 1/2013

Journal of Assisted Reproduction and Genetics 1/2013 Zur Ausgabe

Update Gynäkologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert – ganz bequem per eMail.