nach oben

Journal of Assisted Reproduction and Genetics

Erschienen in:

01.08.2013 | Assisted Reproduction Technologies

Vitrification of blastocysts derived from fair to poor quality cleavage stage embryos can produce high pregnancy rates after warming

verfasst von: Chloë Shaw-Jackson, Evelyne Bertrand, Bénédicte Becker, Jérôme Colin, Caroline Beaudoin-Chabot, Serge Rozenberg, Candice Autin

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 8/2013

Einloggen, um Zugang zu erhalten

Abstract

Purpose

This study investigates whether certain embryos considered unsuitable for cryopreservation on day 3 might nevertheless have the potential to develop into worthwhile blastocysts that could be vitrified in the same cycle.

Methods

Retrospective study: between 2010 and 2011, embryo transfers and cryopreservation took place mainly on day 3 in our centre. Supernumerary embryos of intermediate to poor quality were reassessed on days 5/6 and any good quality blastocysts were vitrified.

Results

Out of 914 cleavage stage (day 3) embryos left in culture, 16 % were vitrified on days 5/6. Fifty blastocyst warming cycles resulted in a 76 % survival rate, 44 % clinical pregnancy rate and 39 % implantation rate. During the same time period, 213 warming cycles of good quality cleavage stage embryos rendered survival rates, clinical pregnancy and implantation rates of 97 %, 23 % and 16 % respectively.

Conclusions

Supernumerary average quality day 3 embryos should be given a second chance to be selected for cryopreservation. If blastocysts are obtained and survive vitrification, there is a good chance of implantation thus reducing embryo waste.

Balaban B, Urman B, Alatas C, Mercan R, Aksoy S, Isiklar A. Blastocyst-stage transfer of poor-quality cleavage-stage embryos results in higher implantation rates. Fertil Steril. 2001;75(3):514–8.PubMedCrossRef

Balaban B, Urman B, Ata B, Isiklar A, Larman MG, Hamilton R, et al. A randomized controlled study of human Day 3 embryo cryopreservation by slow freezing or vitrification: vitrification is associated with higher survival, metabolism and blastocyst formation. Hum Reprod. 2008;23(9):1976–82.PubMedCrossRef

Begley CG. Six red flags for suspect work. Nature. 2013;497(7450):433–4.PubMedCrossRef

Blake DA, Farquhar CM, Johnson N, Proctor M (2011) Cleavage stage versus blastocyst stage embryo transfer in assisted conception. Cochrane Database Syst Rev. Oct 17;(4):CD002118. Review. Update in: Cochrane Database Syst Rev. 2012;7:CD002118.

Capalbo A, Rienzi L, Buccheri M, Maggiulli R, Sapienza F, Romano S, et al. The worldwide frozen embryo reservoir: methodologies to achieve optimal results. Ann N Y Acad Sci. 2011;1221:32–9.PubMedCrossRef

Delvigne A, Kostyla K, Murillo D, Van Hoeck J, Rozenberg S. Oocyte quality and IVF outcome after coasting to prevent ovarian hyper stimulation syndrome. Int J Fertil Womens Med. 2003;48(1):25–31.PubMed

Ebner T, Moser M, Sommergruber M, Gaiswinkler U, Shebl O, Jesacher K, et al. Occurrence and developmental consequences of vacuoles throughout preimplantation development. Fertil Steril. 2005;83(6):1635–40.PubMedCrossRef

Ebner T, Shebl O, Moser M, Sommergruber M, Tews G. Developmental fate of ovoid oocytes. Hum Reprod. 2008;23(1):62–6.PubMedCrossRef

Edgar DH, Gook DA. A critical appraisal of cryopreservation (slow cooling versus vitrification) of human oocytes and embryos. Hum Reprod Update. 2012;18(5):536–54.PubMedCrossRef

10.

Fanchin R, Ayoubi JM, Righini C, Olivennes F, Schonauer LM, Frydman R. Uterine contractility decreases at the time of blastocyst transfers. Hum Reprod. 2001;16(6):1115–9.PubMedCrossRef

11.

Fragouli E, Wells D. Aneuploidy in the human blastocyst. Cytogenet Genome Res. 2011;133(2–4):149–59.PubMedCrossRef

12.

Gardner DK, Schoolcraft WB. In vitro culture of human blastocyst. In: Jansen R, Mortimer D, editors. Towards reproductive certainty: infertility and genetics beyond. Carnforth: Parthernon Press; 1999. p. 378–88.

13.

Graham J, Han T, Porter R, Levy M, Stillman R, Tucker MJ. Day 3 morphology is a poor predictor of blastocyst quality in extended culture. Fertil Steril. 2000;74(3):495–7.PubMedCrossRef

14.

Guerif F, Frapsauce C, Chavez C, Cadoret V, Royere D. Treating women under 36 years old without top-quality embryos on day 2: a prospective study comparing double embryo transfer with single blastocyst transfer. Hum Reprod. 2011;26(4):775–81.PubMedCrossRef

15.

Hammarberg K, Astbury J, Baker H. Women’s experience of IVF: a follow-up study. Hum Reprod. 2001;16(2):374–83.PubMedCrossRef

16.

Hardarson T, Ahlström A, Rogberg L, Botros L, Hillensjö T, Westlander G, et al. Non-invasive metabolomic profiling of Day 2 and 5 embryo culture medium: a prospective randomized trial. Hum Reprod. 2012;27(1):89–96.PubMedCrossRef

17.

Hardarson T, Caisander G, Sjögren A, Hanson C, Hamberger L, Lundin K. A morphological and chromosomal study of blastocysts developing from morphologically suboptimal human pre-embryos compared with control blastocysts. Hum Reprod. 2003;18(2):399–407.PubMedCrossRef

18.

Kader AA, Choi A, Orief Y, Agarwal A. Factors affecting the outcome of human blastocyst vitrification. Reprod Biol Endocrinol. 2009;7:99.PubMedCrossRef

19.

Kolibianikis EM, Zikopoulos K, Verpoest W, Camus M, Joris H, Van Steirteghem AC. Should we advise patients undergoing IVF to start a cycle leading to a day 3 or a day 5 transfer? Hum Reprod. 2004;19(11):2550–4.CrossRef

20.

Kolibianakis EM, Venetis CA, Tarlatzis BC. Cryopreservation of human embryos by vitrification or slow freezing: which one is better? Current Opinion Obstetric Gynecology. 2009;21(3):270–4.CrossRef

21.

Liebermann J. Vitrification of human blastocysts: an update. Reprod Biomed Online. 2009;19 Suppl 4:4328.PubMed

22.

Loutradi KE, Kolibianakis EM, Venetis CA, Papanikolaou EG, Pados G, Bontis I, et al. Cryopreservation of human embryos by vitrification or slow freezing: a systematic review and meta-analysis. Fertil Steril. 2008;90(1):186–93.PubMedCrossRef

23.

Lundin K, Bergh C. Cumulative impact of adding frozen-thawed cycles to single versus double fresh embryo transfers. Reprod Biomed Online. 2007;15(1):76–82.PubMedCrossRef

24.

Magli MC, Gianaroli L, Ferraretti AP, Lappi M, Ruberti A, Farfalli V. Embryo morphology and development are dependent on the chromosomal complement. Fertil Steril. 2007;87(3):534–41.PubMedCrossRef

25.

Manipalviratn S, DeCherney A, Segars J. Imprinting disorders and assisted reproductive technology. Fertil Steril. 2009;91(2):305–15.PubMedCrossRef

26.

Mesut N, Ciray HN, Mesut A, Aksoy T, Bahceci M. Cryopreservation of blastocysts is the most feasible strategy in good responder patients. Fertil Steril. 2011;96(5):1121–5.PubMedCrossRef

27.

Milki AA, Hinckley MD, Gebhardt J, Dasig D, Westphal LM, Behr B. Accuracy of day 3 criteria for selecting the best embryos. Fertil Steril. 2002;77(6):1191–5.PubMedCrossRef

28.

Mukaida T, Oka C, Goto T, Takahashi K. Artificial shrinkage of blastocoels using either a micro-needle or a laser pulse prior to the cooling steps of vitrification improves survival rate and pregnancy outcome of vitrified human blastocysts. Hum Reprod. 2006;21(12):3246–52.PubMedCrossRef

29.

Munné S. Chromosome abnormalities and their relationship to morphology and development of human embryos. Reprod Biomed Online. 2006;12(2):234–53. Review.PubMedCrossRef

30.

Olivius C, Friden B, Borg G, Bergh C. Why do couples discontinue in vitro fertilization treatment? A cohort study. Fertil Steril. 2004;81(2):258–61.PubMedCrossRef

31.

Ren X, Liu Q, Chen W, Zhu G, Li Y, Jin L, et al. Selection and vitrification of embryos with a poor morphological score: a proposal to avoid embryo wastage. J Huazhong Univ Sci Technolog Med Sci. 2012;32(3):405–9. abstract.PubMedCrossRef

32.

Rijnders M, Jansen CA. The predictive value of day 3 embryo morphology regarding blastocyst formation, pregnancy and implantation rate after day 5 transfer following in-vitro fertilization or intracytoplasmic sperm injection. Hum Reprod. 1998;13(10):2869–73.PubMedCrossRef

33.

Racowsky C, Machtinger R. Morphological systems of human embryo assessment and clinical evidence. Reprod Biomed Online. 2013;26(3):210–21.PubMedCrossRef

34.

Ryan GL, Sparks AE, Sipe CS, Syrop CH, Dokras A, Van Voorhis BJ. A mandatory single blastocyst transfer policy with educational campaign in a United States IVF program reduces multiple gestation rates without sacrificing pregnancy rates. Fertil Steril. 2007;88(2):354–60.PubMedCrossRef

35.

Sandalinas M, Sadowy S, Alikani M, Calderon G, Cohen J, Munné S. Developmental ability of chromosomally abnormal human embryos to develop to the blastocyst stage. Hum Reprod. 2001;16(9):1954–8.PubMedCrossRef

36.

Scott Jr RT, Treff NR. Assessing the reproductive competence of individual embryos: a proposal for the validation of new “-omics” technologies. Fertil Steril. 2010;94(3):791–4.PubMedCrossRef

37.

Shapiro BS, Daneshmand ST, Garner FC, Aguirre M, Hudson C, Thomas S. Evidence of impaired endometrial receptivity after ovarian stimulation for in vitro fertilization: a prospective randomized trial comparing fresh and frozen-thawed embryo transfer in normal responders. Fertil Steril. 2011;96(2):344–8.PubMedCrossRef

38.

Shaw JM, Jones GM. Terminology associated with vitrification and other cryopreservation procedures for oocytes and embryos. Hum Reprod Update. 2003;9(6):583–605.PubMedCrossRef

39.

Styer AK, Wright DL, Wolkovich AM, Veiga C, Toth TL. Single-blastocyst transfer decreases twin gestation without affecting pregnancy outcome. Fertil Steril. 2008;89(6):1702–8.PubMedCrossRef

40.

Vajta G, Nagy ZP, Cobo A, Conceicao J, Yovich J. Vitrification in assisted reproduction: myths, mistakes, disbeliefs and confusion. Reprod Biomed Online. 2009;19 Suppl 3:1–7. Review.PubMedCrossRef

41.

Valbuena D, Martin J, de Pablo JL, Remohi J, Pellicer A, Simon C. Increasing levels of estradiol are deleterious to embryonic implantation because they directly affect the embryo. Fertil Steril. 2001;76(5):962–8.PubMedCrossRef

42.

Vanderzwalmen P, Zech N, Greindl AJ, Ectors F, Lejeune B. Cryopreservation of human embryos by vitrification. Gynecol Obstet Fertil. 2006;34(9):760–9.PubMedCrossRef

43.

Vanderzwalmen P, Ectors F, Grobet L, Prapas Y, Panagiotidis Y, Vanderzwalmen S, et al. Aseptic vitrification of blastocysts from infertile patients, egg donors and after IVM. Reprod Biomed Online. 2009;19(5):700–7.PubMedCrossRef

44.

Van Landuyt L, Stoop D, Verheyen G, Verpoest W, Camus M, Van de Velde H, et al. Outcome of closed blastocyst vitrification in relation to blastocyst quality: evaluation of 759 warming cycles in a single-embryo transfer policy. Hum Reprod. 2011;26(3):527–34.PubMedCrossRef

45.

Wong CC, Loewke KE, Bossert NL, Behr B, De Jonge CJ, Baer TM, et al. Non-invasive imaging of human embryos before embryonic genome activation predicts development to the blastocyst stage. Nat Biotechnol. 2010;28(10):1115–21.PubMedCrossRef

46.

Zhu D, Zhang J, Cao S, Zhang J, Heng BC, Huang M, et al. Vitrified-warmed blastocyst transfer cycles yield higher pregnancy and implantation rates compared with fresh blastocyst transfer cycles–time for a new embryo transfer strategy? Fertil Steril. 2011;95(5):1691–5.PubMedCrossRef

47.

Ziebe S, Lundin K, Loft A, Bergh C, Nyboe Andersen A, Selleskog U, et al. FISH analysis for chromosomes 13, 16, 18, 21, 22, X and Y in all blastomeres of IVF pre-embryos from 144 randomly selected donated human oocytes and impact on pre-embryo morphology. Hum Reprod. 2003;18(12):2575–81.PubMedCrossRef

Titel: Vitrification of blastocysts derived from fair to poor quality cleavage stage embryos can produce high pregnancy rates after warming
verfasst von: Chloë Shaw-Jackson
Evelyne Bertrand
Bénédicte Becker
Jérôme Colin
Caroline Beaudoin-Chabot
Serge Rozenberg
Candice Autin
Publikationsdatum: 01.08.2013
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 8/2013
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-013-0037-7

Neu im Fachgebiet Gynäkologie und Geburtshilfe

17.04.2024 | Postoperative nausea and vomiting | Nachrichten

Update Gynäkologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert – ganz bequem per eMail.

Newsletter bestellen

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Vitrification of blastocysts derived from fair to poor quality cleavage stage embryos can produce high pregnancy rates after warming

Abstract

Purpose

Methods

Results

Conclusions

Neu im Fachgebiet Gynäkologie und Geburtshilfe

Hochrisiko-Eingriffe für postoperative Übelkeit

Adjuvante Brustkrebstherapie: Doppelt hält besser

Manche Gestagene können das Risiko für Meningeome erhöhen

Stillende Frauen haben geringeres kardiovaskuläres Risiko

Update Gynäkologie

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

Purpose

Methods

Results

Conclusions

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 8/2013

Slow freezing and vitrification of mouse morula and early blastocysts

EGP: expected gametes performance evaluated by blastocyst culture, a new standard to define “response” from a holistic viewpoint

Connections between preimplantation embryo physiology and culture

Predictors of twin live birth following cryopreserved double embryo transfer on day 3

Short-term storage of tripronucleated human embryos

Nitric oxide modulates mitochondrial activity and apoptosis through protein S-nitrosylation for preimplantation embryo development

Neu im Fachgebiet Gynäkologie und Geburtshilfe

Hochrisiko-Eingriffe für postoperative Übelkeit

Adjuvante Brustkrebstherapie: Doppelt hält besser

Manche Gestagene können das Risiko für Meningeome erhöhen

Stillende Frauen haben geringeres kardiovaskuläres Risiko

Update Gynäkologie