Skip to main content
Erschienen in: Journal of Assisted Reproduction and Genetics 4/2013

01.04.2013 | Technological Innovations

Sperm selection using magnetic activated cell sorting (MACS) in assisted reproduction: a systematic review and meta-analysis

verfasst von: Monica Gil, Valerie Sar-Shalom, Yolisid Melendez Sivira, Ramon Carreras, Miguel Angel Checa

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 4/2013

Einloggen, um Zugang zu erhalten

Abstract

Purpose

To determine whether the use of Magnetic Activated Cell Sorting (MACS) as a sperm selection technique improves ART success rates in couples undergoing assisted reproduction treatment.

Methods

Systematic review and meta-analysis of prospective randomized trials. Two reviewers conducted study selection and data extraction independently.

Results

Five studies (prospective randomized trials) that comprised 499 patients were included. Sperm selection using MACS resulted in statistically significant differences in pregnancy rates when compared with density gradient centrifugation and swim-up techniques (RR = 1.50, 95 % CI 1.14–1.98). No differences were found between the groups according to the implantation (RR = 1.03, 95 % CI 0.80–1.31) and miscarriage (RR = 2.00, 95 % CI 0.19–20.90) rates.

Conclusions

MACS appears to be a safe and efficient method to select functional sperm with consistently good results. This technique may improve pregnancy rates when used to complement standard sperm selection methods in ART.
Literatur
4.
Zurück zum Zitat Aziz N, Said TM, Paasch U, Grunewald S, Sharma RK, Agarwal A. The cause why apoptotic sperm have poor morphology profile as assessed by the sperm deformity index (SDI): a prospective study. Fertil Steril. 2006;86(Issue 3, Suppl 2):S506. doi:10.1016/j.fertnstert.2006.07.1400.CrossRef Aziz N, Said TM, Paasch U, Grunewald S, Sharma RK, Agarwal A. The cause why apoptotic sperm have poor morphology profile as assessed by the sperm deformity index (SDI): a prospective study. Fertil Steril. 2006;86(Issue 3, Suppl 2):S506. doi:10.​1016/​j.​fertnstert.​2006.​07.​1400.CrossRef
5.
Zurück zum Zitat Bochev I, Gavrilov P, Kyurkchiev S, Shterev A, 27th Annual Meeting of the European Society on Human Reproduction and Embryology (ESHRE). Sperm motility improvement after magnetic-activated cell sorting in men with astheno/oligo-asthenozoospermia. Hum Reprod. 2011;26 suppl 1:i133. doi:10.1093/humrep/26.s1.75. Bochev I, Gavrilov P, Kyurkchiev S, Shterev A, 27th Annual Meeting of the European Society on Human Reproduction and Embryology (ESHRE). Sperm motility improvement after magnetic-activated cell sorting in men with astheno/oligo-asthenozoospermia. Hum Reprod. 2011;26 suppl 1:i133. doi:10.​1093/​humrep/​26.​s1.​75.
6.
Zurück zum Zitat Buzzi J, Valcarcel A, Lombardi E, Oses R, Rawe V, Young E, et al. Magnetic activated cell sorting (MACS) improves oocyte donation results associated to severe male factor infertility. Hum Reprod. 2010;25 suppl 1:i118–52. doi:10.1093/humrep/de.25.s1.001. Buzzi J, Valcarcel A, Lombardi E, Oses R, Rawe V, Young E, et al. Magnetic activated cell sorting (MACS) improves oocyte donation results associated to severe male factor infertility. Hum Reprod. 2010;25 suppl 1:i118–52. doi:10.​1093/​humrep/​de.​25.​s1.​001.
8.
Zurück zum Zitat De Vantéry Arrighi C, Hervé L, Chardonnens D, Agostini A. Removal of spermatozoa with externalized phosphatidylserine from sperm preparation in human assisted medical procreation: effects on viability, motility and mitochondrial membrane potential. Reprod Biol Endocrinol. 2009;7(1):1–12. doi:10.1186/1477-7827-7-1.PubMedCrossRef De Vantéry Arrighi C, Hervé L, Chardonnens D, Agostini A. Removal of spermatozoa with externalized phosphatidylserine from sperm preparation in human assisted medical procreation: effects on viability, motility and mitochondrial membrane potential. Reprod Biol Endocrinol. 2009;7(1):1–12. doi:10.​1186/​1477-7827-7-1.PubMedCrossRef
9.
Zurück zum Zitat Dirican EK, Özgün OD, Akarsu S, Okhan A, Özge E, Mukaddes U, et al. Clinical outcome of magnetic activated cell sorting of non-apoptotic spermatozoa before density gradient centrifugation for assisted reproduction. J Assist Reprod Genet. 2008;25:375–81. doi:10.1007/s10815-008-9250-1.PubMedCrossRef Dirican EK, Özgün OD, Akarsu S, Okhan A, Özge E, Mukaddes U, et al. Clinical outcome of magnetic activated cell sorting of non-apoptotic spermatozoa before density gradient centrifugation for assisted reproduction. J Assist Reprod Genet. 2008;25:375–81. doi:10.​1007/​s10815-008-9250-1.PubMedCrossRef
10.
Zurück zum Zitat Duru NK, Morshedi MS, Schuffner A, Oehninger S. Cryopreservation-thawing of fractionated human spermatozoa is associated with membrane phosphatidylserine externalization and not DNA fragmention. J Androl. 2001;22(4):646–51.PubMed Duru NK, Morshedi MS, Schuffner A, Oehninger S. Cryopreservation-thawing of fractionated human spermatozoa is associated with membrane phosphatidylserine externalization and not DNA fragmention. J Androl. 2001;22(4):646–51.PubMed
13.
Zurück zum Zitat Herrero MB, Delbes G, Troueng E, Holzer H, Chan PTK, 28th Annual Meeting of the European Society on Human Reproduction and Embryology (ESHRE). Differential enrichment of sperm with no DNA strand breaks using magnetic activated cell sorting (MACS) in men with various categories of semen parameters. Hum Reprod. 2012;27 suppl 2:ii121–50. doi:10.1093/humrep/27.s2.73.CrossRef Herrero MB, Delbes G, Troueng E, Holzer H, Chan PTK, 28th Annual Meeting of the European Society on Human Reproduction and Embryology (ESHRE). Differential enrichment of sperm with no DNA strand breaks using magnetic activated cell sorting (MACS) in men with various categories of semen parameters. Hum Reprod. 2012;27 suppl 2:ii121–50. doi:10.​1093/​humrep/​27.​s2.​73.CrossRef
14.
Zurück zum Zitat Huang C, Lee T, Chen C, Wu G, Lee C, Lee M, et al. Sperm preparation by magnetic-activated cell sorting improve the sperm-zona pellucida binding capacity and reduces apoptotic sperm. Hum Reprod. 2009;24 suppl 1:2147–i218. Huang C, Lee T, Chen C, Wu G, Lee C, Lee M, et al. Sperm preparation by magnetic-activated cell sorting improve the sperm-zona pellucida binding capacity and reduces apoptotic sperm. Hum Reprod. 2009;24 suppl 1:2147–i218.
15.
17.
Zurück zum Zitat Lee H, Liu C, Lee M. Sperm preparation by magnetic. Activated Cell Sorting (MACS) reduced apoptotic sperm and improved acrosome reaction for unexplained infertility. Fertil Steril. 2009;S143. Lee H, Liu C, Lee M. Sperm preparation by magnetic. Activated Cell Sorting (MACS) reduced apoptotic sperm and improved acrosome reaction for unexplained infertility. Fertil Steril. 2009;S143.
18.
Zurück zum Zitat Lee TH, Liu CH, Shih YT, Tsao HM, Huang CC, Chen HH, et al. Magnetic-activated cell sorting for sperm preparation reduces spermatozoa with apoptotic markers and improves the acrosome reaction in couples with unexplained infertility. Hum Reprod. 2010;25(4):839–46. doi:10.1093/humrep/deq009.PubMedCrossRef Lee TH, Liu CH, Shih YT, Tsao HM, Huang CC, Chen HH, et al. Magnetic-activated cell sorting for sperm preparation reduces spermatozoa with apoptotic markers and improves the acrosome reaction in couples with unexplained infertility. Hum Reprod. 2010;25(4):839–46. doi:10.​1093/​humrep/​deq009.PubMedCrossRef
19.
Zurück zum Zitat Losada C, Ortega I, Pacheco A, Bronet F, 28th Annual Meeting of the European Society on Human Reproduction and Embryology (ESHRE). Can MACS as a sperm preparation technique improve clinic results in patients with Kartagener syndrome? Hum Reprod. 2012;27 suppl 2:ii162–205.CrossRef Losada C, Ortega I, Pacheco A, Bronet F, 28th Annual Meeting of the European Society on Human Reproduction and Embryology (ESHRE). Can MACS as a sperm preparation technique improve clinic results in patients with Kartagener syndrome? Hum Reprod. 2012;27 suppl 2:ii162–205.CrossRef
20.
Zurück zum Zitat Makker K, Agarwal A, Sharma RK. Magnetic activated cell sorting (MACS) Utility in assisted reproduction. Indian J Exp Biol. 2008;46:491–7.PubMed Makker K, Agarwal A, Sharma RK. Magnetic activated cell sorting (MACS) Utility in assisted reproduction. Indian J Exp Biol. 2008;46:491–7.PubMed
21.
Zurück zum Zitat Nadalini M, Tarozzi N, Di Santo M, Borini A, 27th Annual Meeting of the European Society on Human Reproduction and Embryology (ESHRE), Annexin V magnetic-activated cell sorting versus swim-up for the selection of human sperm in ART: is the new approach better then the traditional one? Hum Reprod. 2011;26 suppl 1:i130. Nadalini M, Tarozzi N, Di Santo M, Borini A, 27th Annual Meeting of the European Society on Human Reproduction and Embryology (ESHRE), Annexin V magnetic-activated cell sorting versus swim-up for the selection of human sperm in ART: is the new approach better then the traditional one? Hum Reprod. 2011;26 suppl 1:i130.
23.
Zurück zum Zitat Paasch U, Grunewald S, Fitzl G, Glander HJ. Deterioration of plasma membrane is associated with activated caspases in human spermatozoa. J Androl. 2003;24(2):246–52.PubMed Paasch U, Grunewald S, Fitzl G, Glander HJ. Deterioration of plasma membrane is associated with activated caspases in human spermatozoa. J Androl. 2003;24(2):246–52.PubMed
25.
Zurück zum Zitat Paasch U, Sharma RK, Gupta AK, Grunewald S, Mascha EJ, Thomas A, et al. Cryopreservation and thawing is associated with varying extent of activation of apoptotic machinery in subsets of ejaculated human spermatozoa. Biol Reprod. 2004;71:1828–37. doi:10.1095/biolreprod.103.025627.PubMedCrossRef Paasch U, Sharma RK, Gupta AK, Grunewald S, Mascha EJ, Thomas A, et al. Cryopreservation and thawing is associated with varying extent of activation of apoptotic machinery in subsets of ejaculated human spermatozoa. Biol Reprod. 2004;71:1828–37. doi:10.​1095/​biolreprod.​103.​025627.PubMedCrossRef
26.
Zurück zum Zitat Pesce M, De Felici M. Purification of mouse primordial germ cells by MiniMACS magnetic separation system. Dev Biol. 1995;170:722–5.PubMedCrossRef Pesce M, De Felici M. Purification of mouse primordial germ cells by MiniMACS magnetic separation system. Dev Biol. 1995;170:722–5.PubMedCrossRef
27.
Zurück zum Zitat Polak De Fried E, Denaday F. Single and twin ongoing pregnancies in two cases of previous ART failure after ICSI performed with sperm sorted using annexin V microbeads. Fertility and Sterility. 2010. doi:10.1016/j.fertnstert.2009.12.037. Elsevier Ltd. Polak De Fried E, Denaday F. Single and twin ongoing pregnancies in two cases of previous ART failure after ICSI performed with sperm sorted using annexin V microbeads. Fertility and Sterility. 2010. doi:10.​1016/​j.​fertnstert.​2009.​12.​037. Elsevier Ltd.
28.
Zurück zum Zitat Rawe V. Columnas de anexina como metodología de selección de espermatozoides no apoptóticos durante lCSl. Reproducción Humana. 2009;6(1). Rawe V. Columnas de anexina como metodología de selección de espermatozoides no apoptóticos durante lCSl. Reproducción Humana. 2009;6(1).
29.
Zurück zum Zitat Rawe V, Alvares Sedó C, Uriondo H, Papier S, Miasnik S, Nodar F. ICSI outcome using annexin V columns to select non-apoptotic spermatozoa. Fertil Steril. 2009;92 Suppl 3:S73–4.CrossRef Rawe V, Alvares Sedó C, Uriondo H, Papier S, Miasnik S, Nodar F. ICSI outcome using annexin V columns to select non-apoptotic spermatozoa. Fertil Steril. 2009;92 Suppl 3:S73–4.CrossRef
30.
Zurück zum Zitat Rawe V, Álvarez G, Uriondo H, Papier S, Miasnik S, Nodar F. Separación magnética por columnas de anexinas V: “filtrado molecular” para la selección de espermatozoides no apoptóticos. Reproducción. 2009;24:104–14. Rawe V, Álvarez G, Uriondo H, Papier S, Miasnik S, Nodar F. Separación magnética por columnas de anexinas V: “filtrado molecular” para la selección de espermatozoides no apoptóticos. Reproducción. 2009;24:104–14.
31.
32.
Zurück zum Zitat Robinson L, Gallos ID, Conner SJ, Rajkhowa M, Miller D, Lewis S, et al. The effect of sperm DNA fragmentation on miscarriage rates: a systematic review and meta-analysis. Hum Reprod. 2012;27(10):2908–17. doi:10.1093/humrep/des261.PubMedCrossRef Robinson L, Gallos ID, Conner SJ, Rajkhowa M, Miller D, Lewis S, et al. The effect of sperm DNA fragmentation on miscarriage rates: a systematic review and meta-analysis. Hum Reprod. 2012;27(10):2908–17. doi:10.​1093/​humrep/​des261.PubMedCrossRef
33.
Zurück zum Zitat Romany L, Garrido N, De Los Santos MJ, Aparicio B, Pellicer A, Meseguer M, et al. Effect of sperm selection by anexin-V sorting prior to ICSI in ovum donation program. Preliminary data. Reproductive BioMedicine Online. 2012;24(Suppl1):S2.CrossRef Romany L, Garrido N, De Los Santos MJ, Aparicio B, Pellicer A, Meseguer M, et al. Effect of sperm selection by anexin-V sorting prior to ICSI in ovum donation program. Preliminary data. Reproductive BioMedicine Online. 2012;24(Suppl1):S2.CrossRef
34.
Zurück zum Zitat Romany L, Garrido N, Fernandez JL, Pellicer A, Meseguer M, 28th Annual Meeting of the European Society on Human Reproduction and Embryology (ESHRE). Assisted reproduction results improvements due to magnetic activated cell sorting (MACS) depletion of apoptotic sperm is not mediated by sperm DNA fragmentation decrease. Hum Reprod. 2012;27 suppl 2:ii121–50. doi:10.1093/humrep/27.s2.73.CrossRef Romany L, Garrido N, Fernandez JL, Pellicer A, Meseguer M, 28th Annual Meeting of the European Society on Human Reproduction and Embryology (ESHRE). Assisted reproduction results improvements due to magnetic activated cell sorting (MACS) depletion of apoptotic sperm is not mediated by sperm DNA fragmentation decrease. Hum Reprod. 2012;27 suppl 2:ii121–50. doi:10.​1093/​humrep/​27.​s2.​73.CrossRef
35.
Zurück zum Zitat Romany L, Meseguer M, Garcia-Herrero S, Pellicer A, Garrido N. Magentic activated sorting selection (MACS) of nonapoptotic sperm (NAS) improves pregnancy rates in homologous intrauterine insemination (IUI). Preliminar data. Fertil Steril. 2010;94(4):S14. doi:10.1016/j.fertnstert.2010.07.055. Elsevier Ltd.CrossRef Romany L, Meseguer M, Garcia-Herrero S, Pellicer A, Garrido N. Magentic activated sorting selection (MACS) of nonapoptotic sperm (NAS) improves pregnancy rates in homologous intrauterine insemination (IUI). Preliminar data. Fertil Steril. 2010;94(4):S14. doi:10.​1016/​j.​fertnstert.​2010.​07.​055. Elsevier Ltd.CrossRef
36.
Zurück zum Zitat Romany L, Meseguer M, García-Herrero S, Romero JL, Pellicer A, Garrido N, et al. Magnetic activated sorting of non-apoptotic sperm result in improved embryo quality in ovum donation cycles with intracytoplasmic sperm injection. Hum Reprod. 2010;25 suppl 1:i8. Romany L, Meseguer M, García-Herrero S, Romero JL, Pellicer A, Garrido N, et al. Magnetic activated sorting of non-apoptotic sperm result in improved embryo quality in ovum donation cycles with intracytoplasmic sperm injection. Hum Reprod. 2010;25 suppl 1:i8.
37.
39.
Zurück zum Zitat Said TM, Aziz N, Paasch U, Grunewald S, Glander HJ, Agarwal A. Elimination of Apoptotic Sperm as a Measure for Enhancing Morphological Quality as Assessed by the Sperm Deformity (SDI). Fertil Steril. 2005;84 Suppl 1:448–9.CrossRef Said TM, Aziz N, Paasch U, Grunewald S, Glander HJ, Agarwal A. Elimination of Apoptotic Sperm as a Measure for Enhancing Morphological Quality as Assessed by the Sperm Deformity (SDI). Fertil Steril. 2005;84 Suppl 1:448–9.CrossRef
42.
Zurück zum Zitat San Celestino M, Agudo D, Alonso M, Sanjurjo P, Becerra D, Bronet F, et al. Improved pregnancy rate after sperm magnetic separation technique in egg donation cycles using frozen sperm samples. Hum Reprod. 2011;26 suppl 1:i123–48. San Celestino M, Agudo D, Alonso M, Sanjurjo P, Becerra D, Bronet F, et al. Improved pregnancy rate after sperm magnetic separation technique in egg donation cycles using frozen sperm samples. Hum Reprod. 2011;26 suppl 1:i123–48.
43.
Zurück zum Zitat Tavalee M, Deemeh M, Arbabian M, Nasr-Esfahani M. Density gradient centrifugation before or after magnetic-activated cell sorting: which technique is more useful for clinical sperm selection? J Assit Reprod Genet. 2012;29:31–8. doi:10.1007/s10815-011-9686-6.CrossRef Tavalee M, Deemeh M, Arbabian M, Nasr-Esfahani M. Density gradient centrifugation before or after magnetic-activated cell sorting: which technique is more useful for clinical sperm selection? J Assit Reprod Genet. 2012;29:31–8. doi:10.​1007/​s10815-011-9686-6.CrossRef
45.
Zurück zum Zitat Vermes I, Haanen C, Steffens-Nakken H, Reutelingsperger CP. A novel assay for apoptosis: flow cytometric detection of phosphatidylserine expression of early apoptotic cells using fluorescein labeled Annexin V. J Immunol Methods. 2005;184(1):39–51.CrossRef Vermes I, Haanen C, Steffens-Nakken H, Reutelingsperger CP. A novel assay for apoptosis: flow cytometric detection of phosphatidylserine expression of early apoptotic cells using fluorescein labeled Annexin V. J Immunol Methods. 2005;184(1):39–51.CrossRef
46.
Zurück zum Zitat Vilela M, Tiveron M, Lombardi C, Viglierchio MI, Marconi G, Rawe V, et al. Implantation potential after vitrification of embryos obtained using magnetic active sperm sorting combined with Annexin V during ICSI. Hum Reprod. 2011;26 suppl 1:i200. Vilela M, Tiveron M, Lombardi C, Viglierchio MI, Marconi G, Rawe V, et al. Implantation potential after vitrification of embryos obtained using magnetic active sperm sorting combined with Annexin V during ICSI. Hum Reprod. 2011;26 suppl 1:i200.
47.
Zurück zum Zitat Winkle T, Ditzel N, Gagsteiger F. Possibilities of spremisolation by the MACS-System. Fertil Steril. 2006;86 Suppl 2:130–131.v.CrossRef Winkle T, Ditzel N, Gagsteiger F. Possibilities of spremisolation by the MACS-System. Fertil Steril. 2006;86 Suppl 2:130–131.v.CrossRef
48.
Zurück zum Zitat Young E, De Caro R, Marconi G, Lombardi E, Young E, Tiveron M, et al. Reproductive outcome using Annexin V columns for non-apoptotic sperm selection. Hum Reprod. 2010;25 suppl 1:i6–9. Young E, De Caro R, Marconi G, Lombardi E, Young E, Tiveron M, et al. Reproductive outcome using Annexin V columns for non-apoptotic sperm selection. Hum Reprod. 2010;25 suppl 1:i6–9.
Metadaten
Titel
Sperm selection using magnetic activated cell sorting (MACS) in assisted reproduction: a systematic review and meta-analysis
verfasst von
Monica Gil
Valerie Sar-Shalom
Yolisid Melendez Sivira
Ramon Carreras
Miguel Angel Checa
Publikationsdatum
01.04.2013
Verlag
Springer US
Erschienen in
Journal of Assisted Reproduction and Genetics / Ausgabe 4/2013
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-013-9962-8

Weitere Artikel der Ausgabe 4/2013

Journal of Assisted Reproduction and Genetics 4/2013 Zur Ausgabe

Update Gynäkologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert – ganz bequem per eMail.