nach oben

Journal of Assisted Reproduction and Genetics

Erschienen in:

01.08.2014 | Gamete Biology

Age-associated changes in bovine oocytes and granulosa cell complexes collected from early antral follicles

verfasst von: N. Itami, R. Kawahara-Miki, H. Kawana, M. Endo, T. Kuwayama, H. Iwata

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 8/2014

Einloggen, um Zugang zu erhalten

Abstract

Purpose

To assess the age-associated changes in oocytes and granulosa cells derived from early antral follicles (EAFs).

Method

Gene expression analysis of granulosa cells of the EAFs using a genome analyzer (Illumina) and in vitro culture of oocyte-granulosa cell complexes (OGCs) of EAFs (400–700 μm in diameter) collected from ovaries of aged (>120 months) and young (<50 months) cows.

Results

Gene expression profiles in granulosa cells of EAFs of aged cows, which included changes in genes that encode chaperone proteins and antioxidants. In vivo development of EAFs, as determined by oocyte diameter of EAFs and AFs (3–6 mm in diameter), appeared to be impaired in aged cows and the OGCs of aged cows contained low GSH compared to younger counterparts. When the OGCs were cultured in a medium containing low estradiol (E_2, 0.1 μg/mL), the ratio of antrum formation was higher for OGCs from aged animals than that from young animals, while higher abnormal fertilization rate and lower total cell number of the blastocysts were observed in the OGCs of aged cows compared with those of young cows. On the contrary, when the OGCs were cultured in a medium containing 10 μg/mL E₂, the ratio of antrum formation and fertilization outcome was comparable between the two age groups, whereas the total cell number of the blastocysts was still low in the aged group.

Conclusion

Aging affects the gene expression profiles of the granulosa cells, and impairs in vitro developmental ability of OGCs collected from EAFs.

Nur mit Berechtigung zugänglich

Baerwald AR, Adams GP, Pierson RA. Characterization of ovarian follicular wave dynamics in women. Biol Reprod. 2003;69:1023–31.PubMedCrossRef

Malhi PS, Adams GP, Singh J. Bovine model for the study of reproductive aging in women: follicular, luteal, and endocrine characteristics. Biol Reprod. 2005;73:45–53.PubMedCrossRef

Malhi PS, Adams GP, Pierson RA, Singh J. Bovine model of reproductive aging: response to ovarian synchronization and superstimulation. Theriogenology. 2006;66:1257–66.PubMedCrossRef

Janny L, Menezo YJ. Maternal age effect on early human embryonic development and blastocyst formation. Mol Reprod Dev. 1996;45:31–7.PubMedCrossRef

Scholtes MC, Zeilmaker GH. Blastocyst transfer in day-5 embryo transfer depends primarily on the number of oocytes retrieved and not on age. Fertil Steril. 1998;69:78–83.PubMedCrossRef

Pantos K, Athanasiou V, Stefanidis K, Stavrou D, Vaxevanoglou T, Chronopoulou M. Influence of advanced age on the blastocyst development rate and pregnancy rate in assisted reproductive technology. Fertil Steril. 1999;71:1144–6.PubMedCrossRef

Shapiro BS, Richter KS, Harris DC, Daneshmand ST. Influence of patient age on the growth and transfer of blastocyst-stage embryos. Fertil Steril. 2002;77:700–5.PubMedCrossRef

Malhi PS, Adams GP, Mapletoft RJ, Singh J. Oocyte developmental competence in a bovine model of reproductive aging. Reproduction. 2007;134:233–9.PubMedCrossRef

Yamamoto T, Iwata H, Goto H, Shiratuki S, Tanaka H, Monji Y, et al. Effect of maternal age on the developmental competence and progression of nuclear maturation in bovine oocytes. Mol Reprod Dev. 2010;77:595–604.PubMedCrossRef

10.

Iwata H, Goto H, Tanaka H, Sakaguchi Y, Kimura K, Kuwayama T, et al. Effect of maternal age on mitochondrial DNA copy number, ATP content and IVF outcome of bovine oocytes. Reprod Fertil Dev. 2011;23:424–32.PubMedCrossRef

11.

Takeo S, Goto H, Kuwayama T, Monji Y, Iwata H. Effect of maternal age on the ratio of cleavage and mitochondrial DNA copy number in early developmental stage bovine embryos. J Reprod Dev. 2012;59:174–9.PubMedCentralPubMedCrossRef

12.

Djahanbakhch O, Ezzati M, Zosmer A. Reproductive ageing in women. J Pathol. 2007;211:219–31.PubMedCrossRef

13.

Bancsi LF, Broekmans FJ, Eijkemans MJ, de Jong FH, Habbema JD, te Velde ER. Predictors of poor ovarian response in in vitro fertilization: a prospective study comparing basal markers of ovarian reserve. Fertil Steril. 2002;77:328–36.PubMedCrossRef

14.

Malhi PS, Adams GP, Mapletoft RJ, Singh J. Superovulatory response in a bovine model of reproductive aging. Anim Reprod Sci. 2008;109:100–9.PubMedCrossRef

15.

Xu J, Bernuci MP, Lawson MS, Yeoman RR, Fisher TE, Zelinski MB, et al. Survival, growth, and maturation of secondary follicles from prepubertal, young, and older adult rhesus monkeys during encapsulated three-dimensional culture: effects of gonadotropins and insulin. Reproduction. 2010;140:685–97.PubMedCentralPubMedCrossRef

16.

Choi JK, Ahn JI, Park JH, Lim JM. Derivation of developmentally competent oocytes by in vitro culture of preantral follicles retrieved from aged mice. Fertil Steril. 2011;95:1487–9.PubMedCrossRef

17.

Orisaka M, Tajima K, Tsang BK, Kotsuji F. 2009. Oocyte-granulosa-theca cell interactions during preantral follicular development. J Ovarian Res. 2009;2:9.

18.

Tatone C, Carbone MC, Falone S, Aimola P, Giardinelli A, Caserta D, et al. Age-dependent changes in the expression of superoxide dismutases and catalase are associated with ultrastructural modifications in human granulosa cells. Mol Hum Reprod. 2006;12:655–60.PubMedCrossRef

19.

Hurwitz JM, Jindal S, Greenseid K, Berger D, Brooks A, Santoro N, et al. Reproductive aging is associated with altered gene expression in human uteinizedgranulosa cells. Reprod Sci. 2010;17:56–67.PubMedCrossRef

20.

Ito M, Miyado K, Nakagawa K, Muraki M, Imai M, Yamakawa N, et al. Age-associated changes in the subcellular localization of phosphorylated p38 MAPK in human granulosa cells. Mol Hum Reprod. 2010;16:928–37.PubMedCrossRef

21.

Goto H, Iwata H, Takeo S, Nisinosono K, Murakami S, Monji Y, et al. Effect of bovine age on the proliferative activity, global DNA methylation, relative telomere length and telomerase activity of granulosa cells. Zygote. 2013;21:256–64.PubMedCrossRef

22.

Lim J, Luderer U. Oxidative damage increases and antioxidant gene expression decreases with aging in the mouse ovary. Biol Reprod. 2011;84:775–82.PubMedCentralPubMedCrossRef

23.

Beg MA, Bergfelt DR, Kot K, Wiltbank MC, Ginther OJ. Follicular-fluid factors and granulosa-cell gene expression associated with follicle deviation in cattle. Biol Reprod. 2001;64:432–41.PubMedCrossRef

24.

Endo M, Kawahara-Miki R, Cao F, Kimura K, Kuwayama T, Monji Y, et al. Estradiol supports in vitro development of bovine early antral follicles. Reproduction. 2013;145:85–96.PubMedCrossRef

25.

Hirao Y, Itoh T, Shimizu M, Iga K, Aoyagi K, Kobayashi M, et al. In vitro growth and development of bovine oocyte-granulosa cell complexes on the flat substratum: effects of high polyvinylpyrrolidone concentration in culture medium. Biol Reprod. 2004;70:83–91.PubMedCrossRef

26.

Hirao Y. Conditions affecting growth and developmental competence of mammalian oocytes in vitro. Anim Sci J. 2011;82:187–97.PubMedCrossRef

27.

Takahashi Y, First NL. In vitro development of bovine one-cell embryos: Influence of glucose, lactate, pyruvate, amino acids and vitamins. Theriogenology. 1992;37:963–78.PubMedCrossRef

28.

Takeo S, Kawahara-Miki R, Goto H, Cao F, Kimura K, Monji Y, et al. Age-associated changes in gene expression and developmental competence of bovine oocytes, and a possible countermeasure against age-associated events. Mol Reprod Dev. 2013;80:508–21.PubMedCrossRef

29.

Mortazavi A, Williams BA, McCue K, Schaeffer L, Wold B. Mapping and quantifying mammalian transcriptomes by RNA-Seq. Nat Methods. 2008;5:621–8.PubMedCrossRef

30.

Tasaki H, Iwata H, Sato D, Monji Y, Kuwayama T. Estradiol has a major role in antrum formation of porcine preantral follicles cultured in vitro. Theriogenology. 2013;5:809–14.CrossRef

31.

Greenaway J, Gentry PA, Feige JJ, LaMarre J, Petrik JJ. Thrombospondin and vascular endothelial growth factor are cyclically expressed in an inverse pattern during bovine ovarian follicle development. Biol Reprod. 2005;72:1071–8.PubMedCrossRef

32.

Harlow CR, Bradshaw AC, Rae MT, Shearer KD, Hillier SG. Oestrogen formation and connective tissue growth factor expression in rat granulosa cells. J Endocrinol. 2007;192:41–52.PubMedCrossRef

33.

Assidi M, Dufort I, Ali A, Hamel M, Algriany O, Dielemann S, et al. Identification of potential markers of oocyte competence expressed in bovine cumulus cells matured with follicle-stimulating hormone and/or phorbol myristate acetate in vitro. Biol Reprod. 2008;79:209–22.PubMedCrossRef

34.

Rasmussen LS, Gisvold SE. New author guidelines. Acta Anaesthesiol Scand. 2008;52:594–5.PubMedCrossRef

35.

Chen AQ, Wang ZG, Xu ZR, Yu SD, Yang ZG. Analysis of gene expression in granulosa cells of ovine antral growing follicles using suppressive subtractive hybridization. Anim Reprod Sci. 2009;115:39–48.PubMedCrossRef

36.

Hayashi KG, Ushizawa K, Hosoe M, Takahashi T. Differential genome-wide gene expression profiling of bovine largest and second-largest follicles: identification of genes associated with growth of dominant follicles. Reprod Biol Endocrinol. 2010;5:8–11.

37.

Mora JM, Fenwick MA, Castle L, Baithun M, Ryder TA, Mobberley M, et al. Characterization and significance of adhesion and junction-related proteins in mouse ovarian follicles. Biol Reprod. 2012;153:1–14.

38.

Fargnoli J, Kunisada T, Fornace Jr AJ, Schneider EL, Holbrook NJ. Decreased expたさきssion of heat shock protein 70 mRNA and protein after heat treatment in cells of aged rats. Proc Natl Acad Sci U S A. 1990;87:846–50.PubMedCentralPubMedCrossRef

39.

Pratsinis H, Tsagarakis S, Zervolea I, Giannakopoulos F, Stathakos D, Thalassinos N, Kletsas D. Chronic in vivo exposure to glucocorticoids prolongs cellular lifespan: the case of Cushing’s syndrome-patients’ fibroblasts. 2002;37:1237–45.

40.

Gagliano N, Grizzi F, Annoni G. Mechanisms of aging and liver functions. Dig Dis. 2007;25:118–23.PubMedCrossRef

41.

Velazquez MM, Alfaro NS, Dupuy CR, Salvetti NR, Rey F, Ortega HH. Heat shock protein patterns in the bovine ovary and relation with cystic ovarian disease. Anim Reprod Sci. 2010;118:201–9.PubMedCrossRef

42.

Gola G, Reggiani L, Romanzi F, Beraudo ML. Principle data in the functional treatment of the antero-posterior and vertical dimensions of the arch. Parodontol Stomatol (Nuova). 1985;24:223–32.

43.

Liu Z, Youngquist RS, Garverick HA, Antoniou E. Molecular mechanisms regulating bovine ovarian follicular selection. Mol Reprod Dev. 2009;76:351–66.PubMedCrossRef

44.

Pan X, Zhao J, Zhang WN, Li HY, Mu R, Zhou T, et al. Induction of SOX4 by DNA damage is critical for p53 stabilization and function. Proc Natl Acad Sci U S A. 2009;106:3788–93.PubMedCentralPubMedCrossRef

45.

Qi M, Zhang J, Zeng W, Chen X. DNAJB1 stabilizes MDM2 and contributes to cancer cell proliferation in a p53-dependent manner. Biochim Biophys Acta. 1839;2014:62–9.

46.

Valente P, Fassina G, Melchiori A, Masiello L, Cilli M, Vacca A, et al. TIMP-2 over-expression reduces invasion and angiogenesis and protects B16F10 melanoma cells from apoptosis. Int J Cancer. 1998;19(75):246–53.CrossRef

47.

Stetler-Stevenson WG, Seo DW. TIMP-2: an endogenous inhibitor of angiogenesis. Trends Mol Med. 2005;11:97–103.PubMedCrossRef

48.

Valeri C, Pappalardo S, De Felici M, Manna C. Correlation of oocyte morphometry parameters with woman’s age. J Assist Reprod Genet. 2011;28:545–52.PubMedCentralPubMedCrossRef

49.

Kato S, Endoh H, Masuhiro Y, Kitamoto T, Uchiyama S, Sasaki H, et al. Activation of the estrogen receptor through phosphorylation by mitogen-activated protein kinase. Science. 1995;270:1491–4.PubMedCrossRef

50.

Tschugguel W, Dietrich W, Zhegu Z, Stonek F, Kolbus A, Huber JC. Differential regulation of proteasome-dependent estrogen receptor alpha and beta turnover in cultured human uterine artery endothelial cells. J Clin Endocrinol Metab. 2003;88:2281–7.PubMedCrossRef

51.

Pinzone JJ, Stevenson H, Strobl JS, Berg PE. Molecular and cellular determinants of estrogen receptor alpha expression. Mol Cell Biol. 2004;24:4605–12.PubMedCentralPubMedCrossRef

52.

Soltysik K, Czekaj P. Membrane estrogen receptors - is it an alternative way of estrogen action? J Physiol Pharmacol. 2013;64:129–42.PubMed

53.

Yan J, Suzuki J, Yu X, Kan FW, Qiao J, Chian RC. Cryo-survival, fertilization and early embryonic development of vitrified oocytes derived from mice of different reproductive age. J Assist Reprod Genet. 2010;27:605–11.PubMedCentralPubMedCrossRef

Titel: Age-associated changes in bovine oocytes and granulosa cell complexes collected from early antral follicles
verfasst von: N. Itami
R. Kawahara-Miki
H. Kawana
M. Endo
T. Kuwayama
H. Iwata
Publikationsdatum: 01.08.2014
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 8/2014
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-014-0251-y

Neu im Fachgebiet Gynäkologie und Geburtshilfe

17.04.2024 | Postoperative nausea and vomiting | Nachrichten

Update Gynäkologie

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert – ganz bequem per eMail.

Newsletter bestellen

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Age-associated changes in bovine oocytes and granulosa cell complexes collected from early antral follicles

Abstract

Purpose

Method

Results

Conclusion

Neu im Fachgebiet Gynäkologie und Geburtshilfe

Hochrisiko-Eingriffe für postoperative Übelkeit

Adjuvante Brustkrebstherapie: Doppelt hält besser

Manche Gestagene können das Risiko für Meningeome erhöhen

Stillende Frauen haben geringeres kardiovaskuläres Risiko

Update Gynäkologie

Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

Springer Medizin

Abstract

Purpose

Method

Results

Conclusion

Bitte loggen Sie sich ein, um Zugang zu diesem Inhalt zu erhalten

Weitere Artikel der Ausgabe 8/2014

In vitro culture and characterization of spermatogonial stem cells on Sertoli cell feeder layer in goat (Capra hircus)

Erratum to: Sequential (hFSH + recFSH) vs homogenous (hFSH or recFSH alone) stimulation: clinical and biochemical (cumulus cell gene expression) aspects

Effect of stem cell application on Asherman syndrome, an experimental rat model

Alginate encapsulation supports the growth and differentiation of human primordial follicles within ovarian cortical tissue

Efficacy of ovarian tissue cryopreservation in a major European center

Chromosome distribution of early miscarriages with present or absent embryos: female predominance

Neu im Fachgebiet Gynäkologie und Geburtshilfe

Hochrisiko-Eingriffe für postoperative Übelkeit

Adjuvante Brustkrebstherapie: Doppelt hält besser

Manche Gestagene können das Risiko für Meningeome erhöhen

Stillende Frauen haben geringeres kardiovaskuläres Risiko

Update Gynäkologie