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Journal of Assisted Reproduction and Genetics

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15.09.2020 | Review

Impact on using cryopreservation of testicular or epididymal sperm upon intracytoplasmic sperm injection outcome in men with obstructive azoospermia: a systematic review and meta-analysis

verfasst von: Hanchao Liu, Yun Xie, Linzhi Gao, Xiangzhou Sun, Xiaoyan Liang, Chunhua Deng, Yong Gao, Guihua Liu

Erschienen in: Journal of Assisted Reproduction and Genetics | Ausgabe 11/2020

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Abstract

Purpose

To determine whether there was a significant impact on using cryopreservation of testicular or epididymal sperm upon the outcomes of intracytoplasmic sperm injection (ICSI) in patients with obstructive azoospermia (OA).

Method

Systematic review and meta-analysis of 20 retrospective studies in databases from January 1, 1995, to June 1, 2020.

Result

Twenty articles were included in this study. A total of 3602 (64.1%) of 5616 oocytes injected with fresh epididymal sperm were fertilized, compared with 2366 (61.2%) of 3862 oocytes injected with cryopreserved sperm (relative risk ratio (RR) 0.96, 95% confidence interval (CI) (0.90, 1.02), P > 0.05). A total of 303 (44.1%) of 687 ICSI cycles using fresh epididymal sperm resulted in a clinical pregnancy, compared with 150 (36.6%) of 410 ICSI cycles using cryopreserved epididymal sperm (RR 0.84, 95% CI (0.72, 0.97), P < 0.05). In the testis, a total of 2147 (68.7%) of 3125 oocytes injected with fresh sperm were fertilized, compared with 1623 (63.5%) of 2557 oocytes injected with cryopreserved sperm (RR 0.97, 95% CI (0.90, 1.06), P > 0.05). A total of 151 (47.8%) of 316 ICSI cycles using fresh testicular sperm resulted in a clinical pregnancy, compared with 113 (38.2%) of 296 ICSI cycles using cryopreserved sperm (RR 0.87, 95% CI (0.72, 1.05), P > 0.05).

Conclusions

In men with OA, there was a statistical lower clinical pregnancy rate (CPR) by using frozen epididymal sperm compared with fresh epididymal sperm, but showing no difference on fertilization rate (FR). Additionally, FR and CPR were not affected by whether the retrieved testicular sperm was frozen or fresh.

Nur mit Berechtigung zugänglich

Jarow JP, Espeland MA, Lipshultz LI. Evaluation of the azoospermic patient. J Urol. 1989;142(1):62–5. https://doi.org/10.1016/s0022-5347(17)38662-7.CrossRef

Wosnitzer MS, Goldstein M. Obstructive azoospermia. Urol Clin N Am. 2014;41(1):83–95. https://doi.org/10.1016/j.ucl.2013.08.013.CrossRef

Sperm retrieval for obstructive azoospermia. Fertil Steril. 2008;90(5 Suppl):S213-8. https://doi.org/10.1016/j.fertnstert.2008.08.047.

The management of obstructive azoospermia: a committee opinion. Fertil Steril. 2019;111(5):873-80. https://doi.org/10.1016/j.fertnstert.2019.02.013.

Ohlander S, Hotaling J, Kirshenbaum E, Niederberger C, Eisenberg ML. Impact of fresh versus cryopreserved testicular sperm upon intracytoplasmic sperm injection pregnancy outcomes in men with azoospermia due to spermatogenic dysfunction: a meta-analysis. Fertil Steril. 2014;101(2):344–9. https://doi.org/10.1016/j.fertnstert.2013.10.012.CrossRef

Nicopoullos JD, Gilling-Smith C, Almeida PA, Ramsay JW. The results of 154 ICSI cycles using surgically retrieved sperm from azoospermic men. Hum Reprod. 2004;19(3):579–85. https://doi.org/10.1093/humrep/deh092.CrossRef

Wood S, Thomas K, Schnauffer K, Troup S, Kingsland C, Lewis-Jones I. Reproductive potential of fresh and cryopreserved epididymal and testicular spermatozoa in consecutive intracytoplasmic sperm injection cycles in the same patients. Fertil Steril. 2002;77(6):1162–6. https://doi.org/10.1016/s0015-0282(02)03098-4.CrossRef

Thompson-Cree ME, McClure N, Donnelly ET, Steele KE, Lewis SE. Effects of cryopreservation on testicular sperm nuclear DNA fragmentation and its relationship with assisted conception outcome following ICSI with testicular spermatozoa. Reprod BioMed Online. 2003;7(4):449–55. https://doi.org/10.1016/s1472-6483(10)61889-5.CrossRef

Yu Z, Wei Z, Yang J, Wang T, Jiang H, Li H, et al. Comparison of intracytoplasmic sperm injection outcome with fresh versus frozen-thawed testicular sperm in men with nonobstructive azoospermia: a systematic review and meta-analysis. J Assist Reprod Genet. 2018;35(7):1247–57. https://doi.org/10.1007/s10815-018-1206-5.CrossRef

10.

Nicopoullos JD, Gilling-Smith C, Almeida PA, Norman-Taylor J, Grace I, Ramsay JW. Use of surgical sperm retrieval in azoospermic men: a meta-analysis. Fertil Steril. 2004;82(3):691–701. https://doi.org/10.1016/j.fertnstert.2004.02.116.CrossRef

11.

Silber SJ, Van Steirteghem AC, Liu J, Nagy Z, Tournaye H, Devroey P. High fertilization and pregnancy rate after intracytoplasmic sperm injection with spermatozoa obtained from testicle biopsy. Hum Reprod. 1995;10(1):148–52. https://doi.org/10.1093/humrep/10.1.148.CrossRef

12.

Cota GF, de Sousa MR, Fereguetti TO, Rabello A. Efficacy of anti-leishmania therapy in visceral leishmaniasis among HIV infected patients: a systematic review with indirect comparison. PLoS Negl Trop Dis. 2013;7(5):e2195. https://doi.org/10.1371/journal.pntd.0002195.CrossRef

13.

Liberati A, Altman DG, Tetzlaff J, Mulrow C, Gøtzsche PC, Ioannidis JP, et al. The PRISMA statement for reporting systematic reviews and meta-analyses of studies that evaluate health care interventions: explanation and elaboration. PLoS Med. 2009;6(7):e1000100. https://doi.org/10.1371/journal.pmed.1000100.CrossRef

14.

Lewis S, Clarke M. Forest plots: trying to see the wood and the trees. BMJ. 2001;322(7300):1479–80. https://doi.org/10.1136/bmj.322.7300.1479.CrossRef

15.

Cayan S, Lee D, Conaghan J, Givens CA, Ryan IP, Schriock ED, et al. A comparison of ICSI outcomes with fresh and cryopreserved epididymal spermatozoa from the same couples. Hum Reprod. 2001;16(3):495–9. https://doi.org/10.1093/humrep/16.3.495.CrossRef

16.

Kalsi J, Thum MY, Muneer A, Pryor J, Abdullah H, Minhas S. Analysis of the outcome of intracytoplasmic sperm injection using fresh or frozen sperm. BJU Int. 2011;107(7):1124–8. https://doi.org/10.1111/j.1464-410X.2010.09545.x.CrossRef

17.

Kovac JR, Lehmann KJ, Fischer MA. A single-center study examining the outcomes of percutaneous epididymal sperm aspiration in the treatment of obstructive azoospermia. Urol Ann. 2014;6(1):41–5. https://doi.org/10.4103/0974-7796.127026.CrossRef

18.

Shibahara H, Hamada Y, Hasegawa A, Toji H, Shigeta M, Yoshimoto T, et al. Correlation between the motility of frozen-thawed epididymal spermatozoa and the outcome of intracytoplasmic sperm injection. Int J Androl. 1999;22(5):324–8. https://doi.org/10.1046/j.1365-2605.1999.00187.x.CrossRef

19.

Yu G, Liu Y, Zhang H, Wu K. Application of testicular spermatozoa cryopreservation in assisted reproduction. Int J Gynaecol Obstet. 2018;142(3):354–8. https://doi.org/10.1002/ijgo.12537.CrossRef

20.

Oates RD, Lobel SM, Harris DH, Pang S, Burgess CM, Carson RS. Efficacy of intracytoplasmic sperm injection using intentionally cryopreserved epididymal spermatozoa. Hum Reprod. 1996;11(1):133–8. https://doi.org/10.1093/oxfordjournals.humrep.a019006.CrossRef

21.

Sharma RK, Padron OF, Thomas AJ Jr, Agarwal A. Factors associated with the quality before freezing and after thawing of sperm obtained by microsurgical epididymal aspiration. Fertil Steril. 1997;68(4):626–31. https://doi.org/10.1016/s0015-0282(97)00274-4.CrossRef

22.

Abdel Raheem A, Rushwan N, Garaffa G, Zacharakis E, Doshi A, Heath C, et al. Factors influencing intracytoplasmic sperm injection (ICSI) outcome in men with azoospermia. BJU Int. 2013;112(2):258–64. https://doi.org/10.1111/j.1464-410X.2012.11714.x.CrossRef

23.

Karacan M, Alwaeely F, Erkan S, Çebi Z, Berberoğlugil M, Batukan M, et al. Outcome of intracytoplasmic sperm injection cycles with fresh testicular spermatozoa obtained on the day of or the day before oocyte collection and with cryopreserved testicular sperm in patients with azoospermia. Fertil Steril. 2013;100(4):975–80. https://doi.org/10.1016/j.fertnstert.2013.06.031.CrossRef

24.

Javed A, Ramaiah MK, Talkad MS. ICSI using fresh and frozen PESA-TESA spermatozoa to examine assisted reproductive outcome retrospectively. Obstet Gynecol Sci. 2019;62(6):429–37. https://doi.org/10.5468/ogs.2019.62.6.429.CrossRef

25.

Park YS, Lee SH, Song SJ, Jun JH, Koong MK, Seo JT. Influence of motility on the outcome of in vitro fertilization/intracytoplasmic sperm injection with fresh vs. frozen testicular sperm from men with obstructive azoospermia. Fertil Steril. 2003;80(3):526–30. https://doi.org/10.1016/s0015-0282(03)00798-2.CrossRef

26.

Wu B, Wong D, Lu S, Dickstein S, Silva M, Gelety TJ. Optimal use of fresh and frozen-thawed testicular sperm for intracytoplasmic sperm injection in azoospermic patients. J Assist Reprod Genet. 2005;22(11-12):389–94. https://doi.org/10.1007/s10815-005-7481-y.CrossRef

27.

Devroey P, Silber S, Nagy Z, Liu J, Tournaye H, Joris H, et al. Ongoing pregnancies and birth after intracytoplasmic sperm injection with frozen-thawed epididymal spermatozoa. Hum Reprod. 1995;10(4):903–6. https://doi.org/10.1093/oxfordjournals.humrep.a136058.CrossRef

28.

Nagy Z, Liu J, Cecile J, Silber S, Devroey P, Van Steirteghem A. Using ejaculated, fresh, and frozen-thawed epididymal and testicular spermatozoa gives rise to comparable results after intracytoplasmic sperm injection. Fertil Steril. 1995;63(4):808–15. https://doi.org/10.1016/s0015-0282(16)57486-x.CrossRef

29.

Silber SJ, Devroey P, Tournaye H, Van Steirteghem AC. Fertilizing capacity of epididymal and testicular sperm using intracytoplasmic sperm injection (ICSI). Reprod Fertil Dev. 1995;7(2):281–92; discussion 92-3. https://doi.org/10.1071/rd9950281.CrossRef

30.

Friedler S, Raziel A, Soffer Y, Strassburger D, Komarovsky D, Ron-El R. The outcome of intracytoplasmic injection of fresh and cryopreserved epididymal spermatozoa from patients with obstructive azoospermia--a comparative study. Hum Reprod. 1998;13(7):1872–7. https://doi.org/10.1093/humrep/13.7.1872.CrossRef

31.

Palermo GD, Schlegel PN, Hariprashad JJ, Ergün B, Mielnik A, Zaninovic N, et al. Fertilization and pregnancy outcome with intracytoplasmic sperm injection for azoospermic men. Hum Reprod. 1999;14(3):741–8. https://doi.org/10.1093/humrep/14.3.741.CrossRef

32.

Tournaye H, Merdad T, Silber S, Joris H, Verheyen G, Devroey P, et al. No differences in outcome after intracytoplasmic sperm injection with fresh or with frozen-thawed epididymal spermatozoa. Hum Reprod. 1999;14(1):90–5. https://doi.org/10.1093/humrep/14.1.90.CrossRef

33.

Habermann H, Seo R, Cieslak J, Niederberger C, Prins GS, Ross L. In vitro fertilization outcomes after intracytoplasmic sperm injection with fresh or frozen-thawed testicular spermatozoa. Fertil Steril. 2000;73(5):955–60. https://doi.org/10.1016/s0015-0282(00)00416-7.CrossRef

34.

Tournaye H, Dohle GR, Barratt CL. Fertility preservation in men with cancer. Lancet. 2014;384(9950):1295–301. https://doi.org/10.1016/s0140-6736(14)60495-5.CrossRef

35.

Katz DJ, Kolon TF, Feldman DR, Mulhall JP. Fertility preservation strategies for male patients with cancer. Nat Rev Urol. 2013;10(8):463–72. https://doi.org/10.1038/nrurol.2013.145.CrossRef

36.

Coutifaris C. "Freeze only"--an evolving standard in clinical in vitro fertilization. N Engl J Med. 2016;375(6):577–9. https://doi.org/10.1056/NEJMe1606213.CrossRef

37.

Chen ZJ, Shi Y, Sun Y, Zhang B, Liang X, Cao Y, et al. Fresh versus frozen embryos for infertility in the polycystic ovary syndrome. N Engl J Med. 2016;375(6):523–33. https://doi.org/10.1056/NEJMoa1513873.CrossRef

38.

Mazur P. The role of intracellular freezing in the death of cells cooled at supraoptimal rates. Cryobiology. 1977;14(3):251–72. https://doi.org/10.1016/0011-2240(77)90175-4.CrossRef

39.

Malo C, Elwing B, Soederstroem L, Lundeheim N, Morrell JM, Skidmore JA. Effect of different freezing rates and thawing temperatures on cryosurvival of dromedary camel spermatozoa. Theriogenology. 2019;125:43–8. https://doi.org/10.1016/j.theriogenology.2018.07.037.CrossRef

40.

Fernández-Santos MR, Martínez-Pastor F, García-Macías V, Esteso MC, Soler AJ, Paz P, et al. Sperm characteristics and DNA integrity of Iberian red deer (Cervus elaphus hispanicus) epididymal spermatozoa frozen in the presence of enzymatic and nonenzymatic antioxidants. J Androl. 2007;28(2):294–305. https://doi.org/10.2164/jandrol.106.000935.CrossRef

41.

Agarwal A, Prabakaran SA, Said TM. Prevention of oxidative stress injury to sperm. J Androl. 2005;26(6):654–60. https://doi.org/10.2164/jandrol.05016.CrossRef

42.

Bilodeau JF, Chatterjee S, Sirard MA, Gagnon C. Levels of antioxidant defenses are decreased in bovine spermatozoa after a cycle of freezing and thawing. Mol Reprod Dev. 2000;55(3):282–8. https://doi.org/10.1002/(sici)1098-2795(200003)55:3<282::Aid-mrd6>3.0.Co;2-7.CrossRef

43.

Yildiz C, Ottaviani P, Law N, Ayearst R, Liu L, McKerlie C. Effects of cryopreservation on sperm quality, nuclear DNA integrity, in vitro fertilization, and in vitro embryo development in the mouse. Reproduction. 2007;133(3):585–95. https://doi.org/10.1530/rep-06-0256.CrossRef

44.

Rezaei N, Mohammadi M, Mohammadi H, Khalatbari A, Zare Z. Acrosome and chromatin integrity, oxidative stress, and expression of apoptosis-related genes in cryopreserved mouse epididymal spermatozoa treated with L-Carnitine. Cryobiology. 2020;95:171–6. https://doi.org/10.1016/j.cryobiol.2020.03.006.CrossRef

45.

Ved S, Montag M, Schmutzler A, Prietl G, Haidl G, van der Ven H. Pregnancy following intracytoplasmic sperm injection of immotile spermatozoa selected by the hypo-osmotic swelling-test: a case report. Andrologia. 1997;29(5):241–2. https://doi.org/10.1111/j.1439-0272.1997.tb00477.x.CrossRef

46.

Rogers BJ, Parker RA. Relationship between the human sperm hypo-osmotic swelling test and sperm penetration assay. J Androl. 1991;12(2):152–8.

47.

Stanger JD, Vo L, Yovich JL, Almahbobi G. Hypo-osmotic swelling test identifies individual spermatozoa with minimal DNA fragmentation. Reprod BioMed Online. 2010;21(4):474–84. https://doi.org/10.1016/j.rbmo.2010.06.026.CrossRef

48.

van Wely M, Barbey N, Meissner A, Repping S, Silber SJ. Live birth rates after MESA or TESE in men with obstructive azoospermia: is there a difference? Hum Reprod. 2015;30(4):761–6. https://doi.org/10.1093/humrep/dev032.CrossRef

Titel: Impact on using cryopreservation of testicular or epididymal sperm upon intracytoplasmic sperm injection outcome in men with obstructive azoospermia: a systematic review and meta-analysis
verfasst von: Hanchao Liu
Yun Xie
Linzhi Gao
Xiangzhou Sun
Xiaoyan Liang
Chunhua Deng
Yong Gao
Guihua Liu
Publikationsdatum: 15.09.2020
Verlag: Springer US
Erschienen in: Journal of Assisted Reproduction and Genetics / Ausgabe 11/2020
Print ISSN: 1058-0468
Elektronische ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-020-01940-1

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Impact on using cryopreservation of testicular or epididymal sperm upon intracytoplasmic sperm injection outcome in men with obstructive azoospermia: a systematic review and meta-analysis

Abstract

Purpose

Method

Result

Conclusions

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ICI-Therapie in der Schwangerschaft wird gut toleriert

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Live-Webinar: Aktuelle Leitlinien bei Herz-Kreislauf-Erkrankungen

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Abstract

Purpose

Method

Result

Conclusions

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