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Biomechanical evaluation of regenerating long bone by nanoindentation

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Abstract

It is crucial to measure the mechanical function of regenerating bone in order to assess the mechanical performance of the regenerating portion as well as the efficiency of the regeneration methods. In this study, nanoindentation was applied to regenerating and intact rabbit ulnae to determine the material properties of hardness and elasticity; viscoelasticity was also investigated to precisely evaluate the material properties. Both intact and regenerating bones exhibited remarkable viscoelasticity manifested as a creep behavior during load hold at the maximum load, and the creep was significantly greater in the regenerating bone than the intact bone. The creep resulted in an overestimation of the hardness and Young’s modulus. Hence, during nanoindentation testing of bones, the effect of creep should be eliminated. Moreover, the regenerating bone had lower hardness and Young’s modulus than the intact bone. The nanoindentation technique proved to be a powerful approach for understanding the mechanical properties of regenerating bone.

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Acknowledgments

This work was supported by funds from the “Priority Assistance of the Formation of Worldwide Renowned Centers of Research—The Global COE Program (Project: Center of Excellence for Advanced Structural and Functional Materials Design)” and Grants-in-Aid for Scientific Research from the Japan Society for the Promotion of Science (JSPS) and the Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan.

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Authors and Affiliations

  1. Division of Materials and Manufacturing Science, Graduate School of Engineering, Osaka University, 2-1, Yamada-oka, Suita, Osaka, 565-0871, Japan

    Takuya Ishimoto & Takayoshi Nakano

  2. Institute for Frontier Medical Sciences, Kyoto University, 53, Kawahara-cho, Shogoin, Sakyo-ku, Kyoto, 606-8507, Japan

    Masaya Yamamoto & Yasuhiko Tabata

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  1. Takuya Ishimoto
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  2. Takayoshi Nakano
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  4. Yasuhiko Tabata
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Correspondence to Takayoshi Nakano.

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Ishimoto, T., Nakano, T., Yamamoto, M. et al. Biomechanical evaluation of regenerating long bone by nanoindentation. J Mater Sci: Mater Med 22, 969–976 (2011). https://doi.org/10.1007/s10856-011-4266-y

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  • DOI: https://doi.org/10.1007/s10856-011-4266-y

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