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Repairing defect and preventing collapse of femoral head in a steroid-induced osteonecrotic of femoral head animal model using strontium-doped calcium polyphosphate combined BM-MNCs

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Abstract

We investigated the synergism between strontium-doped calcium polyphosphate (SCPP) and autologous bone marrow mononuclear cells (BM-MNCs) in treating osteonecrosis of the femoral head (ONFH). ONFH was confirmed histopathologically at 2 weeks after methylprednisolone acetate injection and the rabbits were treated with morselized autogenous cancellous compacted bone graft (group I), SCPP combined with BM-MNCs (group II), and calcium polyphosphate (group III), respectively. The amount of newly formed bone in group II increased dramatically by 4, 8, and 12 weeks and much more than that in group III (P < 0.05). VEGF expression in group I was significantly higher than in group II (P = 0.023), and its expression in group II was significantly higher than in group III (P = 0.017). At 12 weeks, group II had articular cartilage collapse and group III had joint-space narrowing. The mean histological and radiological scores for repaired defects in group II were significantly higher than those in group III (P = 0.000) but lower than those in group I (P = 0.000). The implantation of a combination of SCPP and BM-MNCs enhances VEGF expression and promotes osteogenesis, which may improve angiogenesis and allow incorporation and remodeling into new trabecular bone without mechanical weakening.

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Acknowledgments

This research was funded by the National Natural Science Fund of China (Grants 81271976/H0605, 81171763, and 30772202/C1607) and the Sichuan Province Science and Technology Support Program (Grant 2011SZ0027).

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We declare that we have no conflicts of interest.

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Authors and Affiliations

  1. Orthopaedic Department, West China Hospital, Sichuan University, Chengdu, 610041, China

    Pengde Kang, Xiaowei Xie, Zhen Tan, Jing Yang, Bin Shen, Zongke Zhou & Fuxing Pei

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  1. Pengde Kang
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Correspondence to Pengde Kang.

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Kang, P., Xie, X., Tan, Z. et al. Repairing defect and preventing collapse of femoral head in a steroid-induced osteonecrotic of femoral head animal model using strontium-doped calcium polyphosphate combined BM-MNCs. J Mater Sci: Mater Med 26, 80 (2015). https://doi.org/10.1007/s10856-015-5402-x

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  • DOI: https://doi.org/10.1007/s10856-015-5402-x

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