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01.08.2011

Thymic and Bone Marrow Output in Patients with Common Variable Immunodeficiency

verfasst von: Federico Serana, Paolo Airò, Marco Chiarini, Cinzia Zanotti, Mirko Scarsi, Micol Frassi, Vassilios Lougaris, Alessandro Plebani, Luigi Caimi, Luisa Imberti

Erschienen in: Journal of Clinical Immunology | Ausgabe 4/2011

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Abstract

Objective

The study aims to obtain more information about the immune deficit of common variable immunodeficiency (CVID) patients.

Materials and Methods

A new real-time PCR assay was used to quantify T and B lymphocyte mobilization from the production and maturation sites through the detection of T cell receptor excision circles (TRECs) and kappa-deleting recombination circles (KRECs) and to allow the estimation of the average number of B cell divisions. T and B lymphocyte subsets were analyzed by flow cytometry.

Results

The number of TREC⁺ lymphocytes, which depends on age and gender, was significantly reduced in CVID patients. Similarly, KREC concentration was lower than in controls. Classification of patients according to the percentage of memory switched B cells showed that patients belonging to MB2 group and therefore with conserved B cell maturation have the lowest new B cell output but increased average peripheral divisions, leading to the highest B cell number.

Conclusions

TREC and KREC quantification can be helpful for a more complete and informative understanding of a heterogeneous disease such as CVID.

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Titel: Thymic and Bone Marrow Output in Patients with Common Variable Immunodeficiency
verfasst von: Federico Serana
Paolo Airò
Marco Chiarini
Cinzia Zanotti
Mirko Scarsi
Micol Frassi
Vassilios Lougaris
Alessandro Plebani
Luigi Caimi
Luisa Imberti
Publikationsdatum: 01.08.2011
Verlag: Springer US
Erschienen in: Journal of Clinical Immunology / Ausgabe 4/2011
Print ISSN: 0271-9142
Elektronische ISSN: 1573-2592
DOI: https://doi.org/10.1007/s10875-011-9526-6

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Thymic and Bone Marrow Output in Patients with Common Variable Immunodeficiency

Abstract

Objective

Materials and Methods

Results

Conclusions

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