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Free radical scavenging activity of Cleome gynandra L. leaves on adjuvant induced arthritis in rats

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Abstract

The generation of free radicals has been implicated in the causation of several diseases of known and unknown etiologies such as, rheumatoid arthritis, diabetes, cancer, etc., and compounds that can scavenge free radicals have great potential in ameliorating these disease processes. The present study was aimed to investigate the possible anti-oxidant potential of Cleome gynandra leaf extract at a dose of 150 mg/kg body weight for 30 days on adjuvant induced arthritis in experimental rats. Oral administration of C. gynandra leaf extract significantly increased the levels of lipid peroxides and activities of catalase, glutathione peroxidase and decreased the levels of reduced glutathione and superoxide dismutase activity in arthritis induced rats. The free radical scavenging activity of the plant was further evidenced by histological observations made on the limb tissue. The presence of biologically active ingredients and vital trace elements in the leaves readily account for free radical scavenging property of C. gynandra. (Mol Cell Biochem 276: 71–80, 2005)

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Abbreviations

ROS:

reactive oxygen species

RA:

rheumatoid arthritis

NSAIDs:

non steroidal anti-inflammatory drugs

WHO:

World Health Organization

FCA:

Freund’s complete adjuvant

LPO:

lipid peroxidation

TBARS:

thiobarbituric acid reactive substances

SOD:

superoxide dismutase

CAT:

catalase

GPx:

glutathione peroxidase

GSH:

reduced glutathione

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Correspondence to M. Kandaswamy.

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Narendhirakannan, R.T., Subramanian, S. & Kandaswamy, M. Free radical scavenging activity of Cleome gynandra L. leaves on adjuvant induced arthritis in rats. Mol Cell Biochem 276, 71–80 (2005). https://doi.org/10.1007/s11010-005-3234-6

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  • DOI: https://doi.org/10.1007/s11010-005-3234-6

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