Abstract
Partial urinary bladder outlet obstruction mediates cyclic ischemia and reperfusion resulting in the generation of both reactive oxygen species and reactive nitrogen species. It is theorized that with an increase in the level of free radicals, the level of protective antioxidants should decrease. To test this hypothesis, two electron transfer assays, the FRAP method and the CUPRAC method, were used to determine the level of antioxidant reactivity of obstructed and control bladder tissue. The results showed that the CUPRAC assay detected a significant decrease in the reactivity of antioxidants found within the obstructed bladder tissue as compared to the control bladder tissue in both the muscle and mucosa. The FRAP assay did not detect any difference between the muscle and mucosa of the obstructed and control bladder tissue.
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References
Levin RM, Chichester P, Hass MA et al (2000) Obstructive bladder dysfunction: morphological, biochemical, and molecular changes. Eur Urol Suppl 1:14–20. doi:10.1016/S1569-9056(02)00119-7
Hypolite JA, Chang S et al (2006) Partial bladder outlet obstruction induces urethral smooth muscle hypertrophy and decreased force generation. J Urol 175:777–782. doi:10.1016/S0022-5347(05)00138-2
Yang L, He DL et al (2008) Effect of long-term partial bladder outlet obstruction on caldesmon isoforms and their correlation with contractile function. Acta Pharmacol Sin 29:600–605. doi:10.1111/j.1745-7254.2008.00784.x
Kalorin C, Mannikarottu A et al. (2008) Protein oxidation as a novel biomarker of bladder decompensation. Br J Urol 102:494–499
Levin RM, Agartan CA et al (2005) Effect of partial outlet obstruction on nitrotyrosine content and distribution within the rabbit bladder. Mol Cell Biochem 276:143–148. doi:10.1007/s11010-005-4061-5
Siflinger-Birnboim A, Levin RM et al (2008) Partial outlet obstruction of the rabbit urinary bladder induces selective protein oxidation. Neurourol Urodyn 27:532–539. doi:10.1002/nau.20557
Benzie IF, Strain JJ (1996) The ferric reducing ability of plasma (FRAP) as a measure of “antioxidant power”: the FRAP Assay. Anal Biochem 239:70–76. doi:10.1006/abio.1996.0292
Kadkhodaee M, Hemmati M et al (2008) Assessment of plasma antioxidant status in hemodialysis patients. Ther Apher Dial 12:147–151. doi:10.1111/j.1744-9987.2008.00561.x
Apak R, Guclu K et al (2007) Comparative evaluation of various total antioxidant capacity assays applied to phenolic compounds with the CUPRAC assay. Molecules 12:1496–1547. doi:10.3390/12071496
Lin AD, Mannikarottu A et al (2007) Effect of bilateral in vivo ischemia/reperfusion on the activities of superoxide dismutase and catalase: response to a standardized grape suspension. Mol Cell Biochem 296:11–16. doi:10.1007/s11010-005-9068-4
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This material is based upon the work supported in part by the Office of Research and Development Medical Research Service, Department of Veteran’s Affairs, and the Capital Region Medical Research Foundation
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Bean, H., Radu, F., De, E. et al. Comparative evaluation of antioxidant reactivity within obstructed and control rabbit urinary bladder tissue using FRAP and CUPRAC assays. Mol Cell Biochem 323, 139–142 (2009). https://doi.org/10.1007/s11010-008-9972-5
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DOI: https://doi.org/10.1007/s11010-008-9972-5