Abstract
Phosphoinositide 3-kinase (PI3K) pathway exerts its effects through Akt, its downstream target molecule, and thereby regulates various cell functions including cell proliferation, cell transformation, apoptosis, tumor growth, and angiogenesis. Phosphatase and tensin homolog deleted on chromosome 10 (PTEN) has been implicated in regulating cell survival signaling through the PI3K/Akt pathway. However, the mechanism by PI3K/PTEN signaling regulates angiogenesis and tumor growth in vivo remains to be elucidated. Vascular endothelial growth factor (VEGF) plays a pivotal role in tumor angiogenesis. The effect of PTEN on VEGF-mediated signal in pancreatic cancer is unknown. This study aimed to determine the effect of PTEN on both the expression of VEGF and angiogenesis. Toward that end, we used the siRNA knockdown method to specifically define the role of PTEN in the expression of VEGF and angiogenesis. We found that siRNA-mediated inhibition of PTEN gene expression in pancreatic cancer cells increase their VEGF secretion, up-modulated the proliferation, and migration of co-cultured vascular endothelial cell and enhanced tubule formation by HUVEC. In addition, PTEN modulated VEGF-mediated signaling and affected tumor angiogenesis through PI3K/Akt/VEGF/eNOS pathway.
Similar content being viewed by others
References
Tao J, Xiong J, Li T, Yang Z et al (2006) Correlation between protein expression of PTEN in human pancreatic cancer and the proliferation, infiltration, metastasis and prognosis. J Huazhong Univ Sci Technol Med Sci 26:444–447. doi:10.1007/s11596-006-0417-7
Hsu SC, Volpert OV, Steck PA, Mikkelsen T et al (1996) Inhibition of angiogenesis in human glioblastomas by chromosome 10 induction of thrombospondin-1. Cancer Res 56:5684–5691
Stambolic V, Suzuki A (1998) Negative regulation of PKB/Akt-dependent cell survival by the tumor suppressor PTEN. Cell 95:29–39. doi:10.1016/S0092-8674(00)81780-8
Stambolic V, Tsao MS, Macpherson D, Suzuki A et al (2000) High incidence of breast and endometrial neoplasia resembling human Cowden syndrome in pten+/− mice. Cancer Res 60:3605–3611
Liaw D, Marsh DJ, Li J, Dahia PL et al (1997) Germline mutations of the PTEN gene in Cowden disease, an inherited breast and thyroid cancer syndrome. Nat Genet 16:64–67. doi:10.1038/ng0597-64
Marsh DJ, Dahia PL, Zheng Z, Liaw D et al (1997) Germline mutations in PTEN are present in Bannayan-Zonana syndrome. Nat Genet 16:333–334. doi:10.1038/ng0897-333
Chow JY, Quach KT, Cabrera BL, Cabral JA et al (2007) RAS/ERK modulates TGFbeta-regulated PTEN expression in human pancreatic adenocarcinoma cells. Carcinogenesis 28:2321–2327. doi:10.1093/carcin/bgm159
Cantley LC, Neel BG (1999) New insights into tumor suppression: PTEN suppresses tumor formation by restraining the phosphoinositide 3-kinase/AKT pathway. Proc Natl Acad Sci USA 96:4240–4245. doi:10.1073/pnas.96.8.4240
Dong JT, Sipe TW, Hyytinen ER, Li CL, Heise C et al (1998) PTEN/MMAC1 is infrequently mutated in pT2 and pT3 carcinomas of the prostate. Oncogene 17:1979–1982. doi:10.1038/sj.onc.1202119
Simpson L, Parsons R (2001) PTEN: life as a tumor suppressor. Exp Cell Res 264:29–41. doi:10.1006/excr.2000.5130
Alvarez-Nunez F, Bussaglia E, Mauricio D, Ybarra J et al (2006) PTEN promoter methylation in sporadic thyroid carcinomas. Thyroid 16:17–23. doi:10.1089/thy.2006.16.17
Mirmohammadsadegh A, Marini A, Nambiar S, Hassan M et al (2006) Epigenetic silencing of the PTEN gene in melanoma. Cancer Res 66:6546–6552. doi:10.1158/0008-5472.CAN-06-0384
Marsit CJ, Zheng S, Aldape K, Hinds PW et al (2005) PTEN expression in non-small-cell lung cancer: evaluating its relation to tumor characteristics, allelic loss, and epigenetic alteration. Hum Pathol 36:768–776. doi:10.1016/j.humpath.2005.05.006
Wiencke JK, Zheng S, Jelluma N, Tihan T et al (2007) Methylation of the PTEN promoter defines low-grade gliomas and secondary glioblastoma. Neuro-oncology 9:271–279. doi:10.1215/15228517-2007-003
Aoki M, Schetter C, Himly M, Batista O et al (2000) The catalytic subunit of phosphoinositide 3-kinase: requirements for oncogenicity. J Biol Chem 275:6267–6275. doi:10.1074/jbc.275.9.6267
Sakurada A, Suzuki A, Sato M, Yamakawa H et al (1997) Infrequent genetic alterations of the PTEN/MMAC1 gene in Japanese patients with primary cancers of the breast, lung, pancreas, kidney, and ovary. Jpn J Cancer Res 88:1025–1028
Escriva M, Peiro S, Herranz N, Villagrasa P, Dave N et al (2008) Repression of PTEN phosphatase by Snail1 transcriptional factor during gamma radiation-induced apoptosis. Mol Cell Biol 28:1528–1540. doi:10.1128/MCB.02061-07
Wang Q, Zhou Y, Wang X, Chung DH et al (2007) Regulation of PTEN expression in intestinal epithelial cells by c-Jun NH2-terminal kinase activation and nuclear factor-kappaB inhibition. Cancer Res 67:7773–7781. doi:10.1158/0008-5472.CAN-07-0187
Jiang BH, Zheng JZ, Aoki M et al (2000) Phosphatidylinositol 3-kinase signaling mediates angiogenesis and expression of vascular endothelial growth factor in endothelial cells. Proc Natl Acad Sci USA 97:1749–1753. doi:10.1073/pnas.040560897
Ferrara N (2000) Vascular endothelial growth factor and the regulation of angiogenesis. Recent Prog Horm Res 55:15–35 (discussion 35–16)
Thakker GD, Hajjar DP, Muller WA et al (1999) The role of phosphatidylinositol 3-kinase in vascular endothelial growth factor signaling. J Biol Chem 274:10002–10007. doi:10.1074/jbc.274.15.10002
Hamada K, Sasaki T, Koni PA, Natsui M et al (2005) The PTEN/PI3K pathway governs normal vascular development and tumor angiogenesis. Genes Dev 19:2054–2065. doi:10.1101/gad.1308805
Giri D, Ittmann M (1999) Inactivation of the PTEN tumor suppressor gene is associated with increased angiogenesis in clinically localized prostate carcinoma. Hum Pathol 30:419–424. doi:10.1016/S0046-8177(99)90117-X
Wen S, Stolarov J, Myers MP, Su JD et al (2001) PTEN controls tumor-induced angiogenesis. Proc Natl Acad Sci USA 98:4622–4627. doi:10.1073/pnas.081063798
Tahir SA, Yang G, Goltsov AA et al (2008) Tumor cell-secreted caveolin-1 has proangiogenic activity in prostate cancer. Cancer Res 68:731–739. doi:10.1158/0008-5472.CAN-07-2668
Chung BH, Kim CK (2008) Icariin stimulates angiogenesis by activating the MEK/ERK-and PI3K/Akt/eNOS-dependent signal pathway in human endothelial cells. Biochem Biophys Res Commun 376:404–408. doi:10.1016/j.bbrc.2008.09.001
Ferrara N, Chen H, Davis-Smyth T et al (1998) Vascular endothelial growth factor is essential for corpus luteum angiogenesis. Nat Med 4:336–340. doi:10.1038/nm0398-336
Ma J, Sawai H, Matsuo Y, Ochi N et al (2008) IGF-1 mediates PTEN suppression and enhances cell invasion and proliferation via activation of IGF-1/PI3K/Akt signaling pathway in pancreatic cancer cells. J Surg Res. doi:10.1016/j.jss.2008.08.016
Dayanir V, Meyer RD, Lashkari K, Rahimi N (2001) Identification of tyrosine residues in vascular endothelial growth factor receptor-2/FLK-1 involved in activation of phosphatidylinositol 3-kinase and cell proliferation. J Biochem 276:17686–17692
Lee SJ, Kim NamkoongYM, Kim CK, Lee H et al (2006) Fractalkine stimulates angiogenesis by activating the Raf-/MMK/ERK-and PI3K/Akt/eNOS-dependent signal pathway. Am J Physiol Heart Circ Physiol 291:H2836–H2846. doi:10.1152/ajpheart.00113.2006
Papapetropoulos A, Rudic RD, Sessa WC (1999) Molecular control of nitric oxide synthases in the cardiovascular system. Cardiovasc Res 43:509–520. doi:10.1016/S0008-6363(99)00161-3
Dimmeler S, Dernbach E, Zeiher AM (2000) Phosphorylation of the endothelial nitric oxide synthase at ser-1177 is required for VEGF-induced endothelial cell migration. FEBS Lett 477:258–262. doi:10.1016/S0014-5793(00)01657-4
Gomez-Manzano C, Fueyo J, Jiang H, Glass TL et al (2003) Mechanisms underlying PTEN regulation of vascular endothelial growth factor and angiogenesis. Ann Neurol 53:109–117. doi:10.1002/ana.10396
Koul D, Shen R, Garyali A, Ke LD et al (2002) MMAC/PTEN tumor suppressor gene regulates vascular endothelial growth factor-mediated angiogenesis in prostate cancer. Int J Oncol 21:469–475
Huang J, Kontos CD (2002) PTEN modulates vascular endothelial growth factor-mediated signaling and angiogenic effects. J Biol Chem 277:10760–10766. doi:10.1074/jbc.M110219200
Jiang BH, Liu LZ (2008) PI3K/PTEN signaling in tumorigenesis and angiogenesis. Biochim Biophys Acta 1784:150–158
Kontos CD, Stauffer TP, Yang WP, York JD et al (1998) Tyrosine 1101 of Tie2 is the major site of association of p85 and is required for activation of phosphatidylinositol 3-kinase and Akt. Mol Cell Biol 18:4131–4140
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Ma, J., Sawai, H., Ochi, N. et al. PTEN regulate angiogenesis through PI3K/Akt/VEGF signaling pathway in human pancreatic cancer cells. Mol Cell Biochem 331, 161–171 (2009). https://doi.org/10.1007/s11010-009-0154-x
Received:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s11010-009-0154-x