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S100A10 is required for the organization of actin stress fibers and promotion of cell spreading

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Abstract

Dynamic remodeling of the actin cytoskeleton is crucial for biological processes such as cell migration and cell spreading. S100A10 is a member of the S100 protein family and is involved in intracellular trafficking and cell migration. In this study, we examined the role of S100A10 in actin cytoskeletal organization and cell spreading. Depletion of S100A10 induced disruption of stress fiber formation and delay in cell spreading. Rac1 activation during spreading was suppressed by S100A10 knockdown, and exogenous expression of active Rac1 restored the ability of cells to spread in the absence of S100A10. Our results demonstrate the crucial role of S100A10 in actin dynamics promoting cell spreading via Rac1 activation.

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Acknowledgments

We would like to thank the members of the Division of Cancer Biology for helpful discussions. We gratefully acknowledge Dr. Kaibuchi (Department of Cell Pharmacology, Nagoya University Graduate School of Medicine) for providing RhoA and Rac1 plasmids and for technical assistance. This research was partially funded by a Grant from the Ministry of Education, Culture, Sports, Science and Technology of Japan.

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Authors and Affiliations

  1. Division of Cancer Biology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan

    Shurovi Sayeed, Eri Asano, Satoko Ito, Michinari Hamaguchi & Takeshi Senga

  2. Division of Neurogenetics, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya, 466-8550, Japan

    Kinji Ohno

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  1. Shurovi Sayeed
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  2. Eri Asano
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  3. Satoko Ito
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  4. Kinji Ohno
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  5. Michinari Hamaguchi
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  6. Takeshi Senga
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Correspondence to Takeshi Senga.

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Sayeed, S., Asano, E., Ito, S. et al. S100A10 is required for the organization of actin stress fibers and promotion of cell spreading. Mol Cell Biochem 374, 105–111 (2013). https://doi.org/10.1007/s11010-012-1509-2

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  • DOI: https://doi.org/10.1007/s11010-012-1509-2

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