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Reliability of HSP70 (HSPA) expression as a prognostic marker in glioma

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Abstract

Production of heat shock protein 70 (HSP70/HSPA) is induced by a wide range of cellular stress conditions, such as cancer and hypoxia, with production also being linked to tumourigenesis. HSPA mRNA transcripts and proteins were examined in three human glioma cell lines, representing astrocytoma, oligodendroglioma and glioblastoma, plus 18 clinical brain tissue samples. GAPDH was used as a control gene throughout these studies and exhibited a consistent level of expression in a normal astrocyte cell line, tumourous cell lines and tissue samples. In contrast, the average HSPA mRNA copy numbers detected in glioblastoma tissue were between 1.8- and 8.8-fold higher than in lower grade glioma and control tissue, respectively, which is suggestive of a grade-related transcription profile. Similar patterns of grade-related expression were also observed in glioma cell lines. This study indicates for the first time that HSPA expression in glioma cells may possibly be grade related, and hence could have potential as a prognostic marker.

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Acknowledgments

The authors would like to thank the Brain Tumour North West (BTNW) for providing the tissue for this study. This work was supported by a grant from the University of Central Lancashire and Sydney Driscoll Neuroscience Foundation (SDNF).

Conflict of interest

No potential conflicts of interest were disclosed.

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Authors and Affiliations

  1. School of Forensic and Investigative Sciences, University of Central Lancashire, Preston, PR1 2HE, UK

    Glenda Maria Beaman

  2. School of Pharmacy and Biomedical Sciences, University of Central Lancashire, Preston, PR1 2HE, UK

    Sarah R. Dennison

  3. University of Central Lancashire Cyprus, 7080, Larnaca, Cyprus

    Lee K. Chatfield

  4. London South Bank University, 103 Borough Road, London, SE1 0AA, UK

    David A. Phoenix

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  1. Glenda Maria Beaman
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  2. Sarah R. Dennison
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Correspondence to David A. Phoenix.

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Beaman, G.M., Dennison, S.R., Chatfield, L.K. et al. Reliability of HSP70 (HSPA) expression as a prognostic marker in glioma. Mol Cell Biochem 393, 301–307 (2014). https://doi.org/10.1007/s11010-014-2074-7

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  • DOI: https://doi.org/10.1007/s11010-014-2074-7

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