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Genistein ameliorates cardiac inflammation and oxidative stress in streptozotocin-induced diabetic cardiomyopathy in rats

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Abstract

The present study was undertaken to evaluate the protective effects of genistein against cardiac inflammation and oxidative stress in streptozotocin (STZ) (45 mg/kg body weight)-induced diabetic rats. genistein (300 mg/kg/day) was administered orally for 24 weeks to STZ-induced diabetic rats. The effects of genistein on blood glucose, % glycosylated hemoglobin (HbA1c), C-reactive protein, tumor necrosis factor (TNF- α), transforming growth factor (TGF-β1), and total antioxidant were studied. Ultrastructural and histopathological assessment of injury were also undertaken using transmission electron microscope. STZ-induced diabetes resulted in significant increase in the levels of blood glucose, HbA1c, C-reactive protein, TNF- α and TGF-β1, and a decline in total antioxidant reserve of the myocardium. Administration of genistein to diabetic rats resulted in a decrease in blood glucose (p < 0.001),  % HbA1c (p < 0.0001), C-reactive protein (p < 0.001), and expression of TNF- α (p < 0.001) and TGF-β1 (p < 0.0001) proteins. In addition, genistein treatment results in augmentation of total antioxidant (p < 0.01) reserve of the hearts. The above findings were supported by histological as well as immunohistochemical localization of NF-κB (p65) in the heart. Genistein treatment ameliorated the ultrastructural degenerative changes in the cardiac tissues as compared to the diabetic control. The result demonstrates that genistein restored the integrity of the diabetic myocardium by virtue of its anti-inflammatory and antioxidant effects.

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Acknowledgments

Financial support provided by UKIERI and Department of Science and Technology (DST/INT/UK/P-39/2012), India is gratefully acknowledged. Facilities for electron microscopy availed at SAIF (DST), All India Institute of Medical Sciences, New Delhi, are acknowledged.

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Authors and Affiliations

  1. Department of Pharmacology, Delhi Institute of Pharmaceutical Sciences and Research, Pushp Vihar, Sec. -3, New Delhi, 110017, India

    Suresh K. Gupta, Shirish Dongare, Rajani Mathur & Sushma Srivastava

  2. Departments of Pharmacology, MGM Medical College, Navi Mumbai, India

    Ipseeta Ray Mohanty

  3. Department of Pathology, All India Institute of Medical Sciences, New Delhi, India

    Sandeep Mathur

  4. Department of Anatomy, All India Institute of Medical Sciences, New Delhi, India

    Tapas C. Nag

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  1. Suresh K. Gupta
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Gupta, S.K., Dongare, S., Mathur, R. et al. Genistein ameliorates cardiac inflammation and oxidative stress in streptozotocin-induced diabetic cardiomyopathy in rats. Mol Cell Biochem 408, 63–72 (2015). https://doi.org/10.1007/s11010-015-2483-2

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