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Prevalence of hepatitis C virus (HCV) genotypes in Balochistan

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Abstract

A molecular study was conducted to investigate the prevalence of Hepatitis C virus genotypes in HCV infected population of Balochistan. Forty HCV seropositive samples belonging to seven different locations of Balochistan were collected from different health care centres. Qualitative analysis of these samples using PCR resulted in 28 positive samples. The PCR positive samples were subjected to genotyping using the method described by Ohno et al (J Clin Microbiol 35:201–202, 1997) with minor modifications. Genotyping of 28 samples revealed three different genotypes including 3a, 3b and 1a. The most prevalent genotype was 3a with rate of 50% followed by genotype 3b and 1a, respectively. Nine samples remained untyped, suggesting the need of further investigation of genotypes in this region. It has been proposed that sequencing of these samples may be helpful to unreveal these genotypes and further epidemiology of HCV genotypes. Further more, extensive and large scale studies are needed to understand the epidemiology of HCV genotypes, as no such study has been carried in this province.

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References

  1. Bhupinder N, Bhandari MD, Teresa L, Wright MD (1995) Hepatitis C: an overview. Annu Rev Med 46:309–317. doi:10.1146/annurev.med.46.1.309

    Article  Google Scholar 

  2. Hsu H, Wright TL, Luba D (1991) Failure to detect hepatitis C virus genome in human secretion with PCR. Hepatology 14:753–767. doi:10.1002/hep.1840140504

    Article  Google Scholar 

  3. Major ME, Feinstone SM (1997) The molecular virology of Hepatitis C. Hepatology 25:1527–1538. doi:10.1002/hep.510250637

    Article  PubMed  CAS  Google Scholar 

  4. Ohno T, Mezokami M, Wu R, Saleh MG, Ohba K, Orito E et al (1997) New Hepatitis C Virus (HCV) genotyping system that allows for identification of HCV Genotypes 1a, 1b, 2a, 2b, 3a, 3b, 4, 5a, and 6a. J Clin Microbiol 35:201–202

    PubMed  CAS  Google Scholar 

  5. Poynard T, McHutchion JG, Goodman ZD, Ling MH, Albretch J (2000) Is an “A la carte” combination interferon alpha-2b plus ribavirin regimen possible for the first line treatment in patients with chronic Hepatitis C? The ALGOVIRC Project group 31:211–218

    CAS  Google Scholar 

  6. Ogata N, Alter HJ, Miller RH, Purcel RH (1991) Nucleotide sequence and mutation rate of the H strain of Hepatitis C virus. Proc Natl Acad Sci USA 88:3391–3396. doi:10.1073/pnas.88.8.3392

    Article  Google Scholar 

  7. Okamoto H, Kurai K, Okada S, Yamammoto K, Lizuka H, Tanaka T et al (1992) Full-length sequence of a Hepatitis C virus genome having poor homology to reported isolates: comparatively study of four distinct genotypes. Virology 188:331–341. doi:10.1016/0042-6822(92)90762-E

    Article  PubMed  CAS  Google Scholar 

  8. Bukh J, Purcell RH, Miller RH (1994) Sequence analysis of the core gene of 14 Hepatitis C virus genotypes. Proc Natl Acad Sci USA 91:8239–8243. doi:10.1073/pnas.91.17.8239

    Article  PubMed  CAS  Google Scholar 

  9. Ohba K, Mizokami M, Ohno T, Suzuki K, Orito E, Ina Y et al (1995) Classification of Hepatitis C virus into major types and subtypes based on molecular phylogenic tree analysis. Virus Res 36:201–214. doi:10.1016/0168-1702(95)00007-D

    Article  PubMed  CAS  Google Scholar 

  10. Kanai K, Kako M, Okamoto H (1992) HCV genotypes in chronic hepatitis C virus and response to interferon. Lancet 339:1543. doi:10.1016/0140-6736(92)91311-U

    Article  PubMed  CAS  Google Scholar 

  11. Mita E, Hayashi N, Kanazawa Y, Hagiwara H, Ueda K, Kasahara A et al (1994) Hepatitis C virus genotype and RNA titer in the progression of type C chronic liver disease. J Hepatol 21:468–473. doi:10.1016/S0168-8278(05)80330-7

    Article  PubMed  CAS  Google Scholar 

  12. Pozzato G, Moretti M, Franzin F, Croce LS, Tribelli C, Masayu T et al (1991) Severity of liver disease with different Hepatitis C viral clones. Lancet 338:509. doi:10.1016/0140-6736(91)90578-D

    Article  PubMed  CAS  Google Scholar 

  13. Saito I, Miyyamura T, Ohbayashi A, Harada H, Katayama T, Kikuchi S, Watanabe Y, Koi S, Onji M, Ohta Y, Choo LQ, Houghton M, Kuo G (1990) Hepatitis C virus infection is associated with the development of hepatocellular carcinoma. Proc Natl Acad Sci USA 87:6547–6549. doi:10.1073/pnas.87.17.6547

    Article  PubMed  CAS  Google Scholar 

  14. Yoshioka K, Kakumu S, Wakita T, Ishikawa T, Itoh Y, Takayanagi M et al (1992) Detection of hepatitis C virus by polymerase chain reaction and response to inteterferon alpha therapy: relationship to genotypes of Hepatitis C virus. Hepatology 16:293–299. doi:10.1002/hep.1840160203

    Article  PubMed  CAS  Google Scholar 

  15. Khokhar N, Asif N, Khokhar OS (2003) Serotype 3 is most common hepatitis C serotype in Pakistan, however, significant numbers are untypeable. Hepatology 38:270–271. doi:10.1053/jhep.2003.50260

    Article  PubMed  Google Scholar 

  16. Moatter T, Hussainy AS, Hamid S (2002) Comparative analysis of viral inters and histologic features of Pakistani patients infected with HCV type 3. Int J Infect Dis 6:272–276. doi:10.1016/S1201-9712(02)90160-8

    Article  PubMed  Google Scholar 

  17. Das BR, Kundu B, Khandapkar R, Sahani S (2002) Geographical distribution of Hepatitis C virus genotypes in India. Indian J Pathol Microbiol 45:323–328

    PubMed  CAS  Google Scholar 

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Authors and Affiliations

  1. Faculty of Biotechnology and Informatics, BUITEMS, Quetta, Pakistan

    Sarwat Afridi, Muhammad Naeem, Abid Hussain, Naseebullah Kakar & Jamil Ahmad

  2. Department of Molecular Biology & Biotechnology, University of Veterinary and Animal Sciences (UVAS), Lahore, Pakistan

    Masroor Ellahi Babar

Authors
  1. Sarwat Afridi
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  2. Muhammad Naeem
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  3. Abid Hussain
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  4. Naseebullah Kakar
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  5. Masroor Ellahi Babar
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  6. Jamil Ahmad
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Correspondence to Jamil Ahmad.

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Afridi, S., Naeem, M., Hussain, A. et al. Prevalence of hepatitis C virus (HCV) genotypes in Balochistan. Mol Biol Rep 36, 1511–1514 (2009). https://doi.org/10.1007/s11033-008-9342-0

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  • DOI: https://doi.org/10.1007/s11033-008-9342-0

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