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Solid phase subtractive cloning in differentially expressed genes identification

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Abstract

We developed an array-based subtractive hybridization system for one-step high-throughput subtraction. We printed subtractor RNA up to 10.000 times obtaining an excellent contact surface using a little amount of RNA. During hybridization cDNA, common to subtractor and target samples, remains attached to slide immobilized RNA, leaving free in solution target specific cDNA which after retrieval is cloned.

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Authors and Affiliations

  1. Promeridio Association, Non-Profit Research Association, 72018, San Michele Salentino, BR, Italy

    Oscar F. D’Urso

  2. Section of Neurosurgery, Department of Neurosciences, University of Bari Medical School, 70124, Bari, Italy

    Pietro I. D’Urso

  3. Department of Biological and Environmental Sciences and Technologies (DiSTeBA), University of Lecce, Ecotekne, Via Prov.le per Monteroni, 73100, Lecce, Italy

    Carlo Storelli & Santo Marsigliante

Authors
  1. Oscar F. D’Urso
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  2. Pietro I. D’Urso
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  3. Carlo Storelli
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  4. Santo Marsigliante
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Corresponding author

Correspondence to Oscar F. D’Urso.

Additional information

Oscar F. D’Urso, Pietro I. D’Urso contribute equally to this work.

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D’Urso, O.F., D’Urso, P.I., Storelli, C. et al. Solid phase subtractive cloning in differentially expressed genes identification. Mol Biol Rep 37, 1435–1438 (2010). https://doi.org/10.1007/s11033-009-9530-6

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  • DOI: https://doi.org/10.1007/s11033-009-9530-6

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