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Lactobacillus plantarum prevents the development of colitis in IL-10-deficient mouse by reducing the intestinal permeability

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Abstract

It is reported that defects exist in the small intestinal epithelial barrier of inflammatory bowel disease, which might be associated with increased intestinal permeability at a very early stage. Our study aims to investigate the role of Lactobacillus plantarum on the decrease of epithelial permeability and the further protective effects on the intestinal epithelial barrier using the IL-10-deficient mouse model. Our study showed that tight junction associated proteins were increased after the pre-treatment of L. plantarum by fluorescence staining, western blot, real-time PCR and transmission electron microscope. Oral gavage of milk containing L. plantarum was effective in decreasing small intestinal permeability using methods of Ussing chamber assay and sugar probe. Assay of pro-inflammatory cytokines IFN-γ, TNF-α, MPO, and colonic histology by ELISA showed protective effects of L. plantarum on the intestinal epithelial barrier. Therefore, L. plantarum may prevent the development of colitis in IL-10-deficient mice by blocking changes in the expression of TJ proteins, TJ structure and intestinal permeability.

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Acknowledgments

The authors thank Dr. Hang Xiaomin (Institute of Science Life of Only, Shanghai Jiao Tong University, Shanghai, China) for generous provision of Lactobacillus plantarum CGMCC 1258. The authors thank Shanghai Jiao Tong University Affiliated Sixth People’s Hospital for technical assistance during this study. This work was financially supported by National Natural Science Foundation of China (No. 30672044), and the National Basic Research Program of China (No. 2008CB517403).

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Authors and Affiliations

  1. Department of Surgery, Shanghai Jiao Tong University Affiliated Sixth People’s Hospital, 600 Yishan Road, Shanghai, 200233, China

    Zhihua Liu, Peng Zhang, Yanlei Ma, Hongqi Chen, Yukun Zhou, Ming Zhang, Zhaoxin Chu & Huanlong Qin

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  1. Zhihua Liu
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  3. Yanlei Ma
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Correspondence to Huanlong Qin.

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11033_2010_237_MOESM2_ESM.tif

Fig. S1 L. plantarum prevented the injury of tight junction (TJ) and F-actin by fluorescence staining. Line 1 In milk group of IL-10-deficient mice, the fluorescence of F-actin and TJ associated proteins, including occludin, Claudin-1, JAM-1 and ZO-1, was faint, with the fluorescence distributed dispersedly. Lines 2, 3 In the L. plantarum group and AT-1001 group, the intensity of fluorescence and distribution of TJ and F-actin were improved obviously. Line 4 In the group of wild-type mice, TJ and F-actin were continuously distributed with bright green spots along membrane of the cells in the tissue. Original magnification ×2,400 (TIFF 8101 kb)

11033_2010_237_MOESM3_ESM.tif

Fig. S2 L. plantarum protected the ultra-structural of tight junction (TJ) by transmission electron microscope (TEM). A In milk group of IL-10-deficient mice, ultra-structural of TJ was showed as irregular. B, C In the L. plantarum group and AT-1001 group, ultra-structural of TJ was improved obviously. D In the group of wild-type mice, ultra-structural of TJ was shown to be normal (TIFF 4808 kb)

11033_2010_237_MOESM4_ESM.tif

Fig. S3 Small intestinal and colonic permeability were reduced by L. plantarum. A Small intestinal permeability was decreased after given oral gavage of L. plantarum and AT-1001 from 4 weeks and more significant after 8 weeks of age when the small intestinal permeability of IL-10-deficient mice returned to the normal level. B Colonic permeability of IL-10-deficient mice was increased significantly compared with the wild-type; while L. plantarum and AT-1001 had an ameliorating effect after week 10. * P < 0.05. One-way ANOVA was performed with Tukey–Kramer post-hoc comparison. Values were calculated by Student’s t-test. All data are given as mean ± standard deviation (SD) (TIFF 624 kb)

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Liu, Z., Zhang, P., Ma, Y. et al. Lactobacillus plantarum prevents the development of colitis in IL-10-deficient mouse by reducing the intestinal permeability. Mol Biol Rep 38, 1353–1361 (2011). https://doi.org/10.1007/s11033-010-0237-5

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