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Down-regulated energy metabolism genes associated with mitochondria oxidative phosphorylation and fatty acid metabolism in viral cardiomyopathy mouse heart

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Abstract

The majority of experimental and clinical studies indicates that the hypertrophied and failing myocardium are characterized by changes in energy and substrate metabolism that attributed to failing heart changes at the genomic level, in fact, heart failure is caused by various diseases, their energy metabolism and substrate are in different genetic variations, then the potential significance of the molecular mechanisms for the aetiology of heart failure is necessary to be evaluated. Persistent viral infection (especially coxsackievirus group B3) of the myocardium in viral myocarditis and viral dilated cardiomyopathy has never been neglected by experts. This study aimed to explore the role and regulatory mechanism of the altered gene expression for energy metabolism involved in mitochondrial oxidative phosphorylation, fatty acid metabolism in viral dilated cardiomyopathy. cDNA Microarray technology was used to evaluate the expression of >35,852 genes in a mice model of viral dilated cardiomyopathy. In total 1385 highly different genes expression, we analyzed 33 altered genes expression for energy metabolism involved in mitochondrial oxidative phosphorylation, fatty acid metabolism and further selected real-time-PCR for quantity one of regulatory mechanisms for energy including fatty acid metabolism—the UCP2 and assayed cytochrome C oxidase activity by Spectrophotometer to explore mitochondrial oxidative phosphorylation function. We found obviously different expression of 33 energy metabolism genes associated with mitochondria oxidative phosphorylation, fatty acid metabolism in cardiomyopathy mouse heart, the regulatory gene for energy metabolism: UCP2 was down-regulated and cytochrome C oxidase activity was decreased. Genes involved in both fatty acid metabolism and mitochondrial oxidative phosphorylation were down-regulated, mitochondrial uncoupling proteins (UCP2) expression did not increase but decrease which might be a kind of adaptive protection response to regulate energy metabolism for ATP produce.

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Acknowledgment

This work has been funded by the Natural Science Foundation of Heilongjiang Province, China (Contract No. D200982), Education Department projects of Heilongjiang, China (Contract No. 1154G08).

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Authors and Affiliations

  1. Department of Cardiology, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, Heilongjiang, China

    Jing Xu, Hong-gang Nie & Bo Yu

  2. Key Laboratories of Education Ministry for Myocardial Ischemia Mechanism and Treatment, Harbin, 150081, Heilongjiang, China

    Jing Xu, Hong-gang Nie, Xiao-dong Zhang, Ye Tian & Bo Yu

  3. Department of Cardiology, Key Laboratories of Education Ministry for Myocardial Ischemia Mechanism and Treatment, The Second Affiliated Hospital of Harbin Medical University, Harbin, 150081, Heilongjiang, China

    Ye Tian

  4. Department of Cardiology, The Affiliated Hospital of Mudanjiang Medical College, Mudanjiang, 157011, China

    Jing Xu & Xiao-dong Zhang

  5. Department of Pathophysiology, Bio-pharmaceutical Key Laboratory of Heilongjiang Province, Harbin Medical University, Harbin, 150081, China

    Jing Xu & Ye Tian

Authors
  1. Jing Xu
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  2. Hong-gang Nie
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  3. Xiao-dong Zhang
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  4. Ye Tian
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  5. Bo Yu
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Corresponding author

Correspondence to Ye Tian.

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Jing Xu and Hong-gang Nie contributed equally to this work.

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Xu, J., Nie, Hg., Zhang, Xd. et al. Down-regulated energy metabolism genes associated with mitochondria oxidative phosphorylation and fatty acid metabolism in viral cardiomyopathy mouse heart. Mol Biol Rep 38, 4007–4013 (2011). https://doi.org/10.1007/s11033-010-0519-y

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  • DOI: https://doi.org/10.1007/s11033-010-0519-y

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