Abstract
This is a comparative study to isolate the dermatophytes of tinea capitis using the cytobrush and comparing it versus the standard method. A prospective, observational, comparative trial of 178 probable cases of tinea capitis was conducted in two dermatological centers. Each patient underwent mycological tests that included direct exam with KOH and cultures with either of two methods: scraping the scalp to remove hair and cell debris, and the cytobrush. A total of 135 clinically and mycologically proven cases of tinea capitis were included; 119 were non-inflammatory and 16 inflammatory tinea. A total of 131 had a positive direct exam and subsequent primary isolation cultures were obtained in 135 cases. The main dermatophytes isolated were Microsporum canis (68%) and Trichophyton tonsurans (20%). A total of 115/135 (85.1%), were detected with the traditional method, with an average of 11.2 days until positive, while the number detected with the cytobrush was 132/135 (97.7%) with an average of 8.5 days until positive. The chi-square statistical method showed that the cytobrush culture was superior to the standard one with a chi-square of 5.078 (P = 0.025), with a statistically significant difference versus the standard method.
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References
Elewski B. Tinea capitis. Dermatol Clin 1996;14:23–31.
Higgins EM, Fueller LC, Smith CH. Guidelines for the management of tinea capitis. Brit J Dermatol 2000;143:53–8.
Elewski B. Tinea capitis: a current perspective. J Am Acad Derm atol 2000;42:1–20.
Mohrenschlager M, Seidl HP, Ring J, Abeck D. Pediatric tinea capitis: recognition and management. Am J Clin Dermatol 2005;6:203–13.
Sehgal VN, Saxena AK, Kumari S. Tinea capitis. A clinicoetilogic correlation. Int J Dermatol 1985;24:116–9.
Suh DC, Friedlander SF, Raut M, Chang J, Vo L, Shin HC, Tavakkol A. Tinea capitis in the United States: diagnosis, treatment, and costs. J Am Acad Dermatol 2006;55:1111–2.
Al Sogair S, Hay R. Fungal infection in children: tinea capitis. Clin Dermatol 2000; 18:679–85.
Gupta AK, Summerbell RC. Tinea capitis. Med Mycol 2000; 38:255–87.
Mackenzie DWR. Hairbrush diagnosis in detection and eradication of non-fluorescent scalp ringworm. Br Med J 1963;10:363–5.
Hubbard TW, Triquet JM. Brush-culture method for diagnosing tinea capitis. Pediatrics 1992;90:416–8.
Head ES, Henry JC, Macdonald EM. The cotton swab technique for culture of dermatophyte infections, its efficacy and merit. J Am Acad Dermatol 1984;11:797–801.
Friedlander SF, Pickering B, Cunningham BB, Gibbs NF, Eichenfield LF. Use of cotton swab method in diagnosing tinea capitis. Pediatrics. 1999;104:276–9.
Aste N, Pau M, Biggio P. Kerion Celsi: a clinical epidemiological study. Mycoses 1998;41:169–73.
Alvarez MS, Silverberg NB. Tinea capitis. Cutis 2006;78:189–96.
Cremer G, Bournerias I, Vandemeleubroucke E, Houin R, Revuz J. Tinea capitis in adults: misdiagnosis or reappearance? Dermatology 1997;194:8–11.
Medina D, Padilla MC, Fernández R, Arenas R, Bonifaz A. Tiña de la cabeza en adultos: estudio clínico micológico y epidemiológico de 30 casos de la Ciudad de México. Piel 2004;18:403–8.
Babel DE, Baughman SA. Evaluation of the adult carrier state in juvenile tinea capitis caused by Trichophyton tonsurans. J Am Acad Dermatol 1989;21:209–12.
Vargo K, Cohen B. Prevalence of undetected tinea capitis in household members of children with disease. Pediatrics 1993;92:155–6.
Frieden IJ. Tinea capitis: asymptomatic carriage of infection. Pediatr Infect Dis J 1999;18:186–90.
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Bonifaz, A., Isa-Isa, R., Araiza, J. et al. Cytobrush-culture method to diagnose tinea capitis. Mycopathologia 163, 309–313 (2007). https://doi.org/10.1007/s11046-007-9019-6
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DOI: https://doi.org/10.1007/s11046-007-9019-6