Abstract
A new proline-rich polypeptide (PRP-1) has been earlier shown to possess a broad spectrum of biological activities and seems to be a potential medicine. The potential genotoxic properties of PRP-1 and protective effect of PRP-1 against genotoxic action of Mitomycin C (MMC) were analyzed in details in the present work. DNA and chromosome damages were studied in KCL-22 cell line of human myeloid leukemia by the Comet assay and micronucleus induction test, respectively. The results suggest that DNA damages are, at least partly, transient and reparable. PRP-1 at the doses 0.5–2.0 μg/ml does not possess genotoxic activity. Moreover, this peptide expresses both preventive and therapeutic effects against MMC-induced DNA damage. Pre-treatment of cells with PRP-1 also prevents the appearance of daughter cells bearing as heavy MMC-induced DNA/chromosome damages as MNs. Thus, the polypeptide studied is able to protect the cells from genotoxic action of MMC. This defense includes not only DNA but also heritable chromosome damage in post-mitotic cells. Possible mechanisms of PRP-1 protective action are discussed.
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Galoyan AA (1997) Biochemistry of novel cardioactive hormones and immunomodulators of the functional system neurosecretory hypothalamus—endocrine heart. Moscow, Nauka Publishers, p 233
Galoyan AA (2000) Neurochem Res 25:1343–1355
Aprikyan VS, Galoyan KA, Galoyan AA (1998) Neurochem (RAS & NAS RA) 15:189–195
Aprikyan VS, Galoyan AA (1999) Med Sci Armenia 31:29–36
Aprikyan VS, Galoyan KA, Galoyan AA (1999) J Neurochem 77, suppl. S68D
Aprikyan VS, Chailian SG, Galoyan AA (2001) Med Sci Armenia 41:72–77
Galoyan AA (2004) Brain neurosecretory cytokines; immune response and neuronal survival. Kluwer Academic/Plenum Publishers, New York, p 188
Aprikyan VS, Galoyan AA (2000) Neurochimiya 17:60–63
Aprikyan VS, Galoyan AA (2001) Proceedings International Conference “Biochemical and Molecular-Biological Aspects of Brain Immune System”, Yerevan, pp 183–189
Galoyan AA, Aprikyan VS (2002) Neurochem Res 27:305–312
Davtyan TK, Manukyan HM, Hakopyan GS (2005) Neurochem Res 30:297–309
Galoyan AA, Sarkissian JS, Kipriyan TK et al (2000) Neurochem Res 25:1567–1578
Russell WMS, Burch RL (1959) The principles of humane experimental technique. Methuen & Co, London
Singh NP, McCoy MT, Tice RR, Schneider EL (1988) Exp Cell Res 175:184–191
Goethem FV, Lison D, Volders MK (1997) Mutation Res 392:31–43
Galoyan AA, Margaryan KS, Havhannisyan GG et al (2009) Neurochem 3:145–148
Fairbairn DW, Olive PL, O’Neill KL (1995) Mutation Res 339:37–59
Mozaffarieh M, Schoetzau A, Sauter M et al (2008) Mol Vis 14:1584–1588
Fenech, M. F. (1996) Technologies for Detection of DNA Damage and Mutations. In: Pfeifer GP (ed) New York: Plenum Press, Chapter 2, pp 25–36
Fenech MF (2000) Mutation Res 455:81–95
Parry JM (1998) A proposal for a new OECD guideline for the in vitro micronucleus test. URL: http://www.swan.ac.uk/cget/ejgt/article MN UKEMS Protocol.htm
Heddle JA (1973) Mutation Res 18:187–190
Mohanty SK, Rapp A, Monajembashi S et al (2002) Radiation Res 157:378–385
Olive PL, Wlodek D, Banath JP (1991) Cancer Res 51:4671–4676
Merk O, Speit G (1999) Environ Mol Mutagen 33:167–172
Hovhannisyan G, Aroutiounian R, Glei M (2009) Toxicol In Vitro 23:1028–1033
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The research was supported by ISTC grant A-1701.
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Special issue article in honor of Professor Armen Galoyan.
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Aroutiounian, R.M., Hovhannisyan, G.G., Gasparyan, G.H. et al. Proline-rich Polypeptide-1 Protects the Cells In Vitro from Genotoxic Effects of Mitomycin C. Neurochem Res 35, 598–602 (2010). https://doi.org/10.1007/s11064-009-0104-8
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DOI: https://doi.org/10.1007/s11064-009-0104-8