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Neuroprotective Peptide Humanin Inhibits Inflammatory Response in Astrocytes Induced by Lipopolysaccharide

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Abstract

Humanin (HN) has been proved to be an extensive neuroprotective peptide against AD-related and unrelated insults, but little is know about the effect of HN in inflammation response. Current studies indicated the receptors of HN have a close relationship with immune system, which led us to hypothesize HN might have a role in inflammatory response. In this study, we used lipopolysaccharide (LPS) to induce astrocyte inflammation response. This model in vitro allowed us to study the effect of HN on the pure response of astrocyte without the exogenous influence between cells in vivo. Our results showed that 1.0 μg/ml LPS induced a significant activation of astrocyte, shown as the marked increase in the glial fibrillary acidic protein (GFAP) expression, the cell viability and the number of 5-bromo-2′-deoxyuridine (BrdU)-positive living cells. Pretreatment with HN (5, 10, 20 μM) led to a significant inhibition in astrocyte overactivation in a concentration dependent manner. We also found pretreatment with HN decreased the level of proinflammatory cytokines, interleukin (IL)-6, IL-1β and tumor necrosis factor α (TNFα) induced by LPS. Furthermore, we noticed HN couldn’t completely reverse the above inflammatory injury. Our findings imply that HN partly antagonizes inflammation injury induced by LPS and the protective effect of HN on astrocyte is concentration-dependent.

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Acknowledgments

This work was supported by National Natural Science Foundation of China (No. 81100986), Medical Research Foundation of Guangdong Province (No. B2011135), Research Foundation of Education Bureau of Guangzhou (No. 10A154) and Breeding Project of Guangdong Province (No. LYM11105).

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The authors declare that they have no competing interests.

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Authors and Affiliations

  1. Department of Physiology, Guangzhou Medical University, No.195, Dongfeng Road (West), Yuexiu District, Guangzhou, 510182, Guangdong, People’s Republic of China

    Shen-Ting Zhao & Jian-Hua Li

  2. Department of Sports Physiology, Beijing Sport University, Beijing, People’s Republic of China

    Li Zhao

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  1. Shen-Ting Zhao
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  2. Li Zhao
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Correspondence to Jian-Hua Li.

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Zhao, ST., Zhao, L. & Li, JH. Neuroprotective Peptide Humanin Inhibits Inflammatory Response in Astrocytes Induced by Lipopolysaccharide. Neurochem Res 38, 581–588 (2013). https://doi.org/10.1007/s11064-012-0951-6

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  • DOI: https://doi.org/10.1007/s11064-012-0951-6

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