Abstract
Initiation of apoptosis, or programmed cell death, is an important issue in cancer treatment as cancer cells frequently have acquired the ability to block apoptosis and thus are more resistant to chemotherapeutic drugs. Targeted and perhaps selective destruction of cancerous tissue is desirable for many reasons, ranging from the enhancement of or aid to current medical methods to problems currently lacking a solution, i.e., lung cancer. Demonstrated in this publication is the inactivation (killing) of human Melanoma skin cancer cell lines, in vitro, by Floating Electrode Dielectric Barrier Discharge (FE-DBD) plasma. Not only are these cells shown to be killed immediately by high doses of plasma treatment, but low doses are shown to promote apoptotic behavior as detected by TUNEL staining and subsequent flow cytometry. It is shown that plasma acts on the cells directly and not by “poisoning” the solution surrounding the cells, even through a layer of such solution. Potential mechanisms of interaction of plasma with cells are discussed and further steps are proposed to develop an understanding of such systems.
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Notes
In medicine, the term “eschar” describes a slough or dry scab that forms on an area of skin that has been burnt or exposed to corrosive agents. The term “eschar” is commonly confused with “char.”
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Acknowledgements
This work was supported in part by the Defense Advanced Research Projects Agency (DARPA) Award #W81XWH-05-2-0068, National Science Foundation (NSF) Grant #ECS-0304453. Assistance from DARPA program managers Drs. Rick Satava and Jay Lowell is greatly appreciated.
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Fridman, G., Shereshevsky, A., Jost, M.M. et al. Floating Electrode Dielectric Barrier Discharge Plasma in Air Promoting Apoptotic Behavior in Melanoma Skin Cancer Cell Lines. Plasma Chem Plasma Process 27, 163–176 (2007). https://doi.org/10.1007/s11090-007-9048-4
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DOI: https://doi.org/10.1007/s11090-007-9048-4