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Transcriptomic analysis reveals the flooding tolerant mechanism in flooding tolerant line and abscisic acid treated soybean

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Abstract

Soybean is highly sensitive to flooding stress and exhibits markedly reduced plant growth and grain yield under flooding conditions. To explore the mechanisms underlying initial flooding tolerance in soybean, RNA sequencing-based transcriptomic analysis was performed using a flooding-tolerant line and ABA-treated soybean. A total of 31 genes included 12 genes that exhibited similar temporal patterns were commonly changed in these plant groups in response to flooding and they were mainly involved in RNA regulation and protein metabolism. The mRNA expression of matrix metalloproteinase, glucose-6-phosphate isomerase, ATPase family AAA domain-containing protein 1, and cytochrome P450 77A1 was up-regulated in wild-type soybean under flooding conditions; however, no changes were detected in the flooding-tolerant line or ABA-treated soybean. The mRNA expression of cytochrome P450 77A1 was specifically up-regulated in root tips by flooding stress, but returned to the level found in control plants following treatment with the P450 inhibitor uniconazole. The survival ratio and root fresh weight of plants were markedly improved by 3-h uniconazole treatment under flooding stress. Taken together, these results suggest that cytochrome P450 77A1 is suppressed by uniconazole treatment and that this inhibition may enhance soybean tolerance to flooding stress.

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Abbreviations

qRT-PCR:

Quantitative reverse transcription-polymerase chain reaction

ABA:

Abscisic acid

FPKM:

Fragments per kilobase exon per million reads mapped

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Acknowledgements

XY supported by a scholarship from the Chinese Scholarship Council. This work was supported by JSPS KAKENHI Grant Number 15H04445.

Author contributions

Conceived and designed the experiments: XY, SK. Performed the experiments: XY, SK. Analyzed the data: XY, SH, SK. Contributed reagents/materials/analysis tools: XY, SH, MH, MN, SK. Wrote the paper: XY, SH, MH, MN, SK. Assisted with the manuscripts and critically revised it: SK.

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Authors and Affiliations

  1. Graduate School of Life and Environmental Sciences, University of Tsukuba, Tsukuba, 305-8572, Japan

    Xiaojian Yin & Setsuko Komatsu

  2. Institute of Crop Science, National Agriculture and Food Research Organization, Kannondai 2-1-2, Tsukuba, 305-8518, Japan

    Xiaojian Yin, Susumu Hiraga, Makita Hajika & Setsuko Komatsu

  3. Graduate School of Life and Food Sciences, Niigata University, Niigata, 950-2181, Japan

    Minoru Nishimura

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  1. Xiaojian Yin
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  2. Susumu Hiraga
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  3. Makita Hajika
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  4. Minoru Nishimura
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  5. Setsuko Komatsu
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Correspondence to Setsuko Komatsu.

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Yin, X., Hiraga, S., Hajika, M. et al. Transcriptomic analysis reveals the flooding tolerant mechanism in flooding tolerant line and abscisic acid treated soybean. Plant Mol Biol 93, 479–496 (2017). https://doi.org/10.1007/s11103-016-0576-2

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