Abstract
In Poland bread as a staple food both made from wheat and rye flour can be a potential product for future fortification with folic acid. The objective of the study was to examine the effect of fermentation and baking on added folic acid and some endogenous folates stability during breadmaking of rye and wheat breads. Breads were produced using the formulation containing enriched flour with 0.2 mg folic acid/100 g product, baker’s yeast and additionally ascorbic acid for wheat bread and lactic acid for rye bread. Folates were extracted with Hepes/Ches buffer (pH = 7.85) followed by destruction of matrix by amylase and protease and deconjugation with rat serum conjugase. Affinity chromatography (FBP bovine milk) was used to purify and concentrate samples. The folates were separated by HPLC with C18 column and with a combination of fluorescence and UV detection. For both rye and wheat breads there was a decrease of folic acid from flour to bread stage. The total losses depend on baking process and ranged from 12 to 21%. Some changes in the level of different native folate forms during the stage of baking process were also observed.
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References
Stevenson RE, Allen WP, Pai GS, Best R, Seaver LH, Dean J, Thompson S (2000) Decline in prevalence of neural tube defects in high-risk region of the USA. Pediatrics 106: 677–683.
Czeizel AE, Duda I (1992) Prevention of the first occurrence of neural tube defects by periconceptual vitamin supplementation. New England J. of Medicine 327: 32–35.
Bousey CJ, Beresford SA, Omenn GS, Motulsky AG (1995) A quantitative assessment of plasma homocysteine as a risk factor for vascular disease-probable benefits of increasing folic acid intake. J Amer Mad Assoc 274: 1049–1057.
Rampersaud GC, Bailey LB, Kauwell GPA (2002) Relationship of folate to colorectal and cervical cancer: review and recommendations for practitioners. J Am Diet Assoc 102: 1273–1282.
Fishman SM, Christian P, West KP (2000) The role of vitamin in the prevention and control of anemia. Public Health Nutr 3: 125–150.
De Bree A, van Dusseldorp M, Brouwer IA, van Hof KH, Steegers-Theunissen RP (1997) Folate intake in Europe: recommended, actual and desired intake. Eur J Clin Nutr 51: 643–660.
Food and Drug Administration. (1996) Food standards: amendment of standards of identity for enriched grain products to require addition of folic acid 61: 8781–97
Wright AJA, Finglas PM, Southon S (2001) Proposed mandatory fortification of the UK diet with folic acid: have potential risks been underestimated? Trends in Food Science 12: 313–321.
Wolfe CA, Wright DM, Wright AJA, Finglas PM (2004) Impact of folate and homocysteine status of twenty-eight day supplementation with a non-alcoholic beer naturally high in folate on healthy male volunteers consuming a free-living diet. In: Brzozowska A, Finglas PM, Wright D, Araucz M (eds), First International Conference on Folate Analysis, Bioavailability and Health, Warsaw, Poland, February 11–14, pp 128–129.
Pfeiffer CM, Rogers LM, Gregory III JF (1997) Determination of folate in cereal-grain products using trienzyme extraction and combined affinity and reversed-phase liquid chromatography. J Agric Food Chem 45: 407–413.
Konings EJM (1999) A validated liquid chromatographic method for determining folates in vegetables, milk powder, liver, and flour. Journal of AOAC International 82: 119–127.
Blakley RL (1969) The Biochemistry of folic acid and related pteridines. North-Holland Publishing Company, Amsterdam-London, pp 92–94.
Wilson SD, Horne DW (1984) High-performance liquid chromatographic determination of the distribution of naturally occurring folic derivatives in rat liver. Anal Biochem 142: 529–535.
Tamura T (1998) Determination of food folate. Nutritional Biochemistry 9: 285–293.
Yon M, Hyun TH (2003) Folate content of food commonly consumed in Korea measured after trienzyme extraction. Nutrition Research 23: 735–746.
Horubalowa A, Haber T (1994) Analiza Techniczna w Piekarstwie. Wydawnictwa Szkolne i Pedagogiczne, Warsaw, pp 152–165, in polish.
Selhub J, Ahmad O, Rosenberg IH (1980) Preparation and use of affinity chromatography with bovine milk folate-binding protein covalently linked to Sepharose 4B. Methods Enzymol 66: 686–690.
Osseyi EO, Wehling RL, Albrecht JA (2001) HPLC determination of stability and distribution of added folic acid and some endogenous folates during breadmaking. Cereal Chem 78: 375–378.
Kariluoto S, Vahteristo L, Salovaara H, Katina K, Liukkonen KH, Piironen V (2004) Effect of baking method and fermentation on folate content of rye and wheat breads. Cereal Chem 81: 134–139.
Seyoum E, Selhub J (1998) Properties of food folates determined by stability and susceptibility to intestinal pteroylpolyglutamate hydrolase action. J Nutr 128: 1956–1960.
Kariluoto MS, Vahteristo LT, Piironen VI (2001) Applicability of microbiological assay chromatography purification followed by high-performance liquid chromatography (HPLC) in studying folate contents in rye. J Sci Food Agri 81: 938–942.
Vahteristo LT, Lehikoinen KE, Ollilainen V, Koivistoinen PE, Varo P (1998) Oven-baking and frozen storage affect folate vitamer retention. Lebensm-Wiss Technol 31: 329–333.
Seyoum E, Selhub J (1993) Combined affinity and ion pair column chromatographies for the analysis of food folate. J Nutr Biochem 4: 488–494.
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Gujska, E., Majewska, K. Effect of Baking Process on Added Folic Acid and Endogenous Folates Stability in Wheat and Rye Breads. Plant Foods Hum Nutr 60, 37–42 (2005). https://doi.org/10.1007/s11130-005-5097-0
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DOI: https://doi.org/10.1007/s11130-005-5097-0